{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:14:41Z","timestamp":1788268481811,"version":"build-2803163510"},"reference-count":46,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T00:00:00Z","timestamp":1622160000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Zebrafish"],"published-print":{"date-parts":[[2021,6,1]]},"abstract":"<jats:p>\n                    Intestinal neoplasms and preneoplastic lesions are common in zebrafish research facilities. Previous studies have demonstrated that these neoplasms are caused by a transmissible agent, and two candidate agents have been implicated: a\n                    <jats:italic toggle=\"yes\">Mycoplasma<\/jats:italic>\n                    sp. related to\n                    <jats:italic toggle=\"yes\">Mycoplasma penetrans<\/jats:italic>\n                    and the intestinal parasitic nematode,\n                    <jats:italic toggle=\"yes\">Pseudocapillaria tomentosa<\/jats:italic>\n                    , and both agents are common in zebrafish facilities. To elucidate the role of these two agents in the occurrence and severity of neoplasia and other intestinal lesions, we conducted two experimental inoculation studies. Exposed fish were examined at various time points over an 8-month period for intestinal histopathologic changes and the burden of\n                    <jats:italic toggle=\"yes\">Mycoplasma<\/jats:italic>\n                    and nematodes. Fish exposed to\n                    <jats:italic toggle=\"yes\">Mycoplasma<\/jats:italic>\n                    sp. isolated from zebrafish were associated with preneoplastic lesions. Fish exposed to the nematode alone or with the\n                    <jats:italic toggle=\"yes\">Mycoplasma<\/jats:italic>\n                    isolate developed severe lesions and neoplasms. Both inflammation and neoplasm scores were associated with an increase in\n                    <jats:italic toggle=\"yes\">Mycoplasma<\/jats:italic>\n                    burden. These results support the conclusions that\n                    <jats:italic toggle=\"yes\">P. tomentosa<\/jats:italic>\n                    is a strong promoter of intestinal neoplasms in zebrafish and that\n                    <jats:italic toggle=\"yes\">Mycoplasma<\/jats:italic>\n                    alone can also cause intestinal lesions and accelerate cancer development in the context of nematode infection.\n                  <\/jats:p>","DOI":"10.1089\/zeb.2020.1955","type":"journal-article","created":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T16:53:54Z","timestamp":1621270434000},"page":"207-220","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":25,"title":["<i>Pseudocapillaria tomentosa<\/i>\n                    ,\n                    <i>Mycoplasma<\/i>\n                    spp., and Intestinal Lesions in Experimentally Infected Zebrafish\n                    <i>Danio rerio<\/i>"],"prefix":"10.1177","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7968-5142","authenticated-orcid":false,"given":"Michael L.","family":"Kent","sequence":"first","affiliation":[{"name":"Department of Microbiology and Oregon State University, Corvallis, Oregon, USA."},{"name":"Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena S.","family":"Wall","sequence":"additional","affiliation":[{"name":"Department of Biology and Institute of Molecular Biology, Eugene, University of Oregon, Eugene, Oregon, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Sichel","sequence":"additional","affiliation":[{"name":"Department of Biology and Institute of Molecular Biology, Eugene, University of Oregon, Eugene, Oregon, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Watral","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Oregon State University, Corvallis, Oregon, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keaton","family":"Stagaman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Oregon State University, Corvallis, Oregon, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Sharpton","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Oregon State University, Corvallis, Oregon, USA."},{"name":"Department of Statistics, Oregon State University, Corvallis, Oregon, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Guillemin","sequence":"additional","affiliation":[{"name":"Department of Biology and Institute of Molecular Biology, Eugene, University of Oregon, Eugene, Oregon, USA."},{"name":"Humans and the Microbiome Program, CIFAR, Toronto, Ontario, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,5,28]]},"reference":[{"key":"e_1_3_4_2_2","unstructured":"Cartner SC Eisen JS Farmer SC Guillemin KJ Kent ML Sanders GE (eds): The Zebrafish in Biomedical Research: Biology Husbandry Diseases and Research Applications. 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Cartner S Eisen J Farmer S Guillemin K Kent ML and Sanders GS (eds) pp. 527\u2013546 American College of Laboratory Animal Medicine Series Elsevier San Diego 2020.","DOI":"10.1016\/B978-0-12-812431-4.00043-9"},{"key":"e_1_3_4_7_2","first-page":"362","article-title":"Pseudocapillaria tomentosa, a nematode pathogen of zebrafish (Danio rerio) kept in research colonies and associated neoplasms","volume":"52","author":"Kent ML","year":"2002","unstructured":"Kent ML, , Bishop-Stewart JK, , Matthews JL, , Spitsbergen JM. Pseudocapillaria tomentosa, a nematode pathogen of zebrafish (Danio rerio) kept in research colonies and associated neoplasms. Comp Med, 2002; 52:362\u2013367.","journal-title":"Comp Med"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","unstructured":"Kent ML Sanders JL: Important parasites in research facilities. Chapter 40. In: The Zebrafish in Biomedical Research: Biology Husbandry Diseases and Research Applications. 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