{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T21:43:57Z","timestamp":1773351837090,"version":"3.50.1"},"reference-count":24,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Zebrafish"],"published-print":{"date-parts":[[2023,12,1]]},"abstract":"<jats:p>\n                    Synthetic cathinones are drugs of abuse substituted for amphetamine-like stimulant drugs such as methamphetamine. In this study, methamphetamine was studied as a prototypical amphetamine-like drug as a first step toward establishing methods to study this entire drug class. The internal concentration of methamphetamine in zebrafish larvae was determined using matrix-matched calibration along with extraction and purification of samples using the quick, easy, cheap, effective, rugged, and safe technique in liquid chromatography\u2013tandem mass spectrometry. Whole-body and head\/trunk uptake and elimination in 5-day postfertilization zebrafish larvae were determined. A gradient method was developed using 5\u2009mM ammonium formate with 0.1% formic acid and methanol with 0.1% formic acid as mobile phases, 10\u2009min of total run time, and a 0.3\u2009mL\/min flow rate. The limit of quantification was 60\u2009ng\/mL, linearity with\n                    <jats:italic toggle=\"yes\">r<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.9991, and recovery values from 92% to 120%. The internal concentration of methamphetamine was quantifiable in whole-body homogenates within 15\u2009min of uptake analysis. The internal concentration increased with time, whereas a biphasic elimination pattern was shown. With increasing length of exposure, a higher accumulation of drugs was found in the head than in the trunk.\n                  <\/jats:p>","DOI":"10.1089\/zeb.2023.0022","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T09:52:16Z","timestamp":1703065936000},"page":"236-242","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of Liquid Chromatography\u2013Tandem Mass Spectrometry Analytical Methods for the Study of Whole-Body, Head, and Trunk Uptake and Elimination of Methamphetamine in 5-Day Postfertilization Zebrafish Larvae Using the Quick, Easy, Cheap, Effective, Rugged, and Safe Technique"],"prefix":"10.1177","volume":"20","author":[{"given":"Kabita","family":"Gwachha","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander S.","family":"Wisner","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, Ohio, USA."},{"name":"Department of Medicinal and Biological Chemistry, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tue Quynh","family":"Chau","sequence":"additional","affiliation":[{"name":"Department of Medicinal and Biological Chemistry, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank Scott","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isaac T.","family":"Schiefer","sequence":"additional","affiliation":[{"name":"Department of Medicinal and Biological Chemistry, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick E.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"key":"e_1_3_4_2_2","unstructured":"UNODC. 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Cognitive deficits and synthetic khat-related cathinones. In: Handbook of Substance Misuse and Addictions: From Biology to Public Health. Springer; 2022; pp. 1681\u20131703.","DOI":"10.1007\/978-3-030-92392-1_86"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2013.00109"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfw156"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00414"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2017.06.075"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2017.1541"},{"issue":"27","key":"e_1_3_4_17_2","first-page":"1618","article-title":"LC-MS\/MS method for the determination of organophosphorus pesticides and their metabolites in salmon and zebrafish fed with plant-based feed ingredients","volume":"411","author":"Garlito Molina B","year":"2019","unstructured":"Garlito Molina B, , Ib\u00e1\u00f1ez M, , Portoles T, et al. LC-MS\/MS method for the determination of organophosphorus pesticides and their metabolites in salmon and zebrafish fed with plant-based feed ingredients. Anal Bioanal Chem, 2019; 411(27):1618\u20132642; doi: 10.1007\/s00216-019-02104-6.","journal-title":"Anal Bioanal Chem"},{"key":"e_1_3_4_18_2","unstructured":"Heeren S. Lethal and Toxic Effects of Synthetic Cathinone Analogues at Physiologically Preferred and High Temperatures. Master's thesis The University of Toledo; 2021."},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkx057"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.11.025"},{"key":"e_1_3_4_21_2","unstructured":"Committee for Medicinal Products for Human Use. Guideline on Bioanalytical Method Validation. 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