{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T22:19:15Z","timestamp":1789510755371,"version":"build-2803163510"},"reference-count":65,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Zebrafish"],"published-print":{"date-parts":[[2024,6,1]]},"abstract":"<jats:p>\n                    In fish species, heterochromatinization is one process that could trigger sex chromosome differentiation. The present article describes a nascent XX\/XY sex chromosome system evidenced by heterochromatin accumulation and microsatellite (GATA)\n                    <jats:sub>8<\/jats:sub>\n                    in\n                    <jats:italic toggle=\"yes\">Hypostomus albopunctatus<\/jats:italic>\n                    from two populations of the Paran\u00e1 River basin. The specimens of\n                    <jats:italic toggle=\"yes\">H. albopunctatus<\/jats:italic>\n                    from the Campo and Bossi Rivers share the same karyotype. The species exhibits 74 chromosomes (8m+14sm +16st +36a, fundamental number\u2009=\u2009112). The C-banding technique suggests male heterogamety in\n                    <jats:italic toggle=\"yes\">H. albopunctatus<\/jats:italic>\n                    , where the Y-chromosome is morphologically like the X-chromosome but differs from it for having long arms that are entirely heterochromatic. Double fluorescence\n                    <jats:italic toggle=\"yes\">in situ<\/jats:italic>\n                    hybridization (FISH) with 18S and 5S rDNA probes confirmed the Ag-nucleolus organizer region sites in a single pair for both populations, and minor rDNA clusters showed interpopulational variation. FISH with the microsatellite (GATA)\n                    <jats:sub>8<\/jats:sub>\n                    probe showed a dispersed pattern in the karyotype, accumulating these sequences of sex chromosomes of both populations. FISH with microsatellite (CGC)\n                    <jats:sub>10<\/jats:sub>\n                    probe showed interpopulational variation. The absence of differentiated sex chromosomes in\n                    <jats:italic toggle=\"yes\">H. albopunctatus<\/jats:italic>\n                    is described previously, and a new variant is documented herein where XY chromosomes can be seen in an early stage of differentiation.\n                  <\/jats:p>","DOI":"10.1089\/zeb.2023.0100","type":"journal-article","created":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T12:28:00Z","timestamp":1708604880000},"page":"265-273","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Initial Steps of XY Sex Chromosome Differentiation in the Armored Catfish\n                    <i>Hypostomus albopunctatus<\/i>\n                    (Siluriformes: Loricariidae) Revealed by Heterochromatin Accumulation"],"prefix":"10.1177","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1502-8408","authenticated-orcid":false,"given":"Ligia Carla","family":"Balini","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Genetics, and Cell Biology, State University of Maring\u00e1, Maring\u00e1, Paran\u00e1, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9905-1507","authenticated-orcid":false,"given":"Carlos Alexandre","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Genetics, and Cell Biology, State University of Maring\u00e1, Maring\u00e1, Paran\u00e1, Brazil."},{"name":"Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological Sciences Center, State University of Maring\u00e1, Maring\u00e1, Paran\u00e1, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0881-0345","authenticated-orcid":false,"given":"Ana Luiza de Brito","family":"Portela-Castro","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Genetics, and Cell Biology, State University of Maring\u00e1, Maring\u00e1, Paran\u00e1, Brazil."},{"name":"Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological Sciences Center, State University of Maring\u00e1, Maring\u00e1, Paran\u00e1, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6975-6346","authenticated-orcid":false,"given":"Rafael Fernando de","family":"Melo","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Genetics, and Cell Biology, State University of Maring\u00e1, Maring\u00e1, Paran\u00e1, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5642-4908","authenticated-orcid":false,"given":"Cl\u00e1udio Henrique","family":"Zawadzki","sequence":"additional","affiliation":[{"name":"Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological Sciences Center, State University of Maring\u00e1, Maring\u00e1, Paran\u00e1, Brazil."},{"name":"Department of Biology, State University of Maring\u00e1, Maring\u00e1, Paran\u00e1, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1990-4803","authenticated-orcid":false,"given":"Luciana Andreia","family":"Borin-Carvalho","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Genetics, and Cell Biology, State University of Maring\u00e1, Maring\u00e1, Paran\u00e1, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,6,17]]},"reference":[{"key":"e_1_3_5_2_2","unstructured":"Nelson JS Grande TC Wilson MVH. 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