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Based on a previously published technique, we simplified the process by applying an anticoagulant solution directly to the incision site. The modified protocol involves immersing the fish in an ice bath, making a cross-sectional incision, and immediately applying anticoagulant solution. Centrifugation of the specimens provides a streamlined and efficient approach to zebrafish fluid sample collection, compatible with classic biochemical marker analyses.<\/jats:p>","DOI":"10.1089\/zeb.2023.0101","type":"journal-article","created":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T12:28:00Z","timestamp":1708604880000},"page":"259-264","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Modified Procedure for Simple and Rapid Collection of Zebrafish Fluid Samples for Biochemical Analyses"],"prefix":"10.1177","volume":"21","author":[{"given":"Ana Paula Apolin\u00e1rio da","family":"Silva","sequence":"first","affiliation":[{"name":"Laboratory of Biotechnology and Molecular Biology, Department of Nutrition, Northeast Biotechnology Network (RENORBIO), State University of Cear\u00e1, Fortaleza, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Xavier da","family":"Silva Neto","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Molecular Biology, Department of Nutrition, Northeast Biotechnology Network (RENORBIO), State University of Cear\u00e1, Fortaleza, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Ytalo Gomes da","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Molecular Biology, Department of Nutrition, Northeast Biotechnology Network (RENORBIO), State University of Cear\u00e1, Fortaleza, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Lorena Pereira","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Molecular Biology, Department of Nutrition, Northeast Biotechnology Network (RENORBIO), State University of Cear\u00e1, Fortaleza, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Bezerra da","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Molecular Biology, Department of Nutrition, Northeast Biotechnology Network (RENORBIO), State University of Cear\u00e1, Fortaleza, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Izabel Florindo","family":"Guedes","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Molecular Biology, Department of Nutrition, Northeast Biotechnology Network (RENORBIO), State University of Cear\u00e1, Fortaleza, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,6,17]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-021-00571-5"},{"issue":"63","key":"e_1_3_5_3_2","first-page":"3865","article-title":"Blood collection for biochemical analysis in adult zebrafish","author":"Pedroso GL","year":"2012","unstructured":"Pedroso GL, , Hammes TO, , Escobar TDC, et al. 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