{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T22:37:42Z","timestamp":1770763062409,"version":"3.50.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2002,12]]},"abstract":"\n Methicillin-resistant\n Staphylococcus aureus<\/jats:italic>\n (MRSA) isolates recovered from a general hospital in Oporto, Portugal, during two periods (1992-1993 and 1996-2000) were characterized by pulsed-field\n gel electrophoresis (PFGE) of\n Sma<\/jats:italic>\n I fragments, and by hybridization of\n Cla<\/jats:italic>\n I digests with\n mecA<\/jats:italic>\n and Tn\n 554<\/jats:italic>\n probes, discriminating the isolates in\n mecA<\/jats:italic>\n ::Tn\n 554<\/jats:italic>\n ::PFGE\n genotypes. In addition, a representative sample of the defined genotypes was characterized by multilocus sequence typing (MLST) and SCC\n mec<\/jats:italic>\n (staphylococcal cassette chromosome\n mec<\/jats:italic>\n ) typing,\n generating the corresponding ST-SCC\n mec<\/jats:italic>\n types. In 1992-1993, 77% of MRSA belonged to the Iberian clone (genotype I::E::A or ST247-IA). In 1996-2000, the frequency of this clone decreased to 19% and\n the majority (69%) of the isolates belonged to another international clone, the Brazilian MRSA (genotype XI::B::B or ST239-IIIA). Trimethoprim\/sulfamethoxazole (SXT) was confirmed to be an important phenotypic\n marker to distinguish the Iberian (SXT-susceptible) and the Brazilian (SXT-resistant) clones in MRSA isolates from Portugal. Our observations document major shifts in the dominant MRSA clonal types that\n occurred in this hospital since 1992, suggesting a selective advantage of the Brazilian relatively to the Iberian clone. In addition to these two MRSA clones that are the most frequent in Portuguese hospitals\n since the early 1990s, sporadic MRSA clones (representing 14% of the total) were identified and characterized.\n <\/jats:p>","DOI":"10.1089\/10766290260469561","type":"journal-article","created":{"date-parts":[[2003,1,16]],"date-time":"2003-01-16T20:22:20Z","timestamp":1042748540000},"page":"301-309","source":"Crossref","is-referenced-by-count":30,"title":["Clonal and Antibiotic Resistance Profiles of Methicillin-Resistant\n Staphylococcus aureus<\/i>\n (MRSA) from a Portuguese Hospital over Time"],"prefix":"10.1177","volume":"8","author":[{"given":"M.L.","family":"Amorim","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa (ITQB\/UNL), Oeiras, Portugal; Unidade de Biologia Molecular and Servi\u00e7o de Microbiologia, Hospital Geral de Santo Ant\u00f3nio, Porto, Portugal"}]},{"given":"M.","family":"Aires de Sousa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa (ITQB\/UNL), Oeiras, Portugal"}]},{"given":"I. Santos","family":"Sanches","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa (ITQB\/UNL), Oeiras, Portugal and Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa (FCT\/UNL), Monte da Caparica, Portugal"}]},{"given":"R.","family":"S\u00e1-Le\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa (ITQB\/UNL), Oeiras, Portugal"}]},{"given":"J.M.","family":"Cabeda","sequence":"additional","affiliation":[{"name":"Unidade de Biologia Molecular, Hospital Geral de Santo Ant\u00f3nio, Porto, Portugal"}]},{"given":"J.M.","family":"Amorim","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Microbiologia, Hospital Geral de Santo Ant\u00f3nio, Porto, Portugal"}]},{"given":"H.","family":"de Lencastre","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa (ITQB\/UNL), Oeiras, Portugal and Laboratory of Microbiology, The Rockefeller University, New York, NY 10021"}]}],"member":"179","published-online":{"date-parts":[[2002,12]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/10766290260469561","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/10766290260469561","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:14:07Z","timestamp":1770747247000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/10766290260469561"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,12]]}},"alternative-id":["10.1089\/10766290260469561"],"URL":"https:\/\/doi.org\/10.1089\/10766290260469561","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,12]]}}}