{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T02:15:38Z","timestamp":1779156938674,"version":"3.51.4"},"reference-count":33,"publisher":"American Society for Cell Biology (ASCB)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:p>Mammalian LGN\/AGS3 proteins and their Drosophila Pins orthologue are cytoplasmic regulators of G-protein signaling. In Drosophila, Pins localizes to the lateral cortex of polarized epithelial cells and to the apical cortex of neuroblasts where it plays important roles in their asymmetric division. Using overexpression studies in different cell line systems, we demonstrate here that, like Drosophila Pins, LGN can exhibit enriched localization at the cell cortex, depending on the cell cycle and the culture system used. We find that in WISH, PC12, and NRK but not COS cells, LGN is largely directed to the cell cortex during mitosis. Overexpression of truncated protein domains further identified the G\u03b1-binding C-terminal portion of LGN as a sufficient domain for cortical localization in cell culture. In mitotic COS cells that normally do not exhibit cortical LGN localization, LGN is redirected to the cell cortex upon overexpression of G\u03b1 subunits of heterotrimeric G-proteins. The results also show that the cortical localization of LGN is dependent on microfilaments and that interfering with LGN function in cultured cell lines causes early disruption to cell cycle progression.<\/jats:p>","DOI":"10.1091\/mbc.e03-04-0212","type":"journal-article","created":{"date-parts":[[2003,5,6]],"date-time":"2003-05-06T00:16:26Z","timestamp":1052180186000},"page":"3144-3155","source":"Crossref","is-referenced-by-count":49,"title":["Subcellular Localization of LGN During Mitosis: Evidence for Its Cortical Localization in Mitotic Cell Culture Systems and Its Requirement for Normal Cell Cycle Progression"],"prefix":"10.1091","volume":"14","author":[{"given":"Rachna","family":"Kaushik","sequence":"first","affiliation":[{"name":"Institute of Molecular and Cell Biology, Singapore 117609"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengwei","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology, Singapore 117609"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Chia","sequence":"additional","affiliation":[{"name":"MRC Centre for Developmental Neurobiology, King's College London, London SE1 1UL, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohang","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology, Singapore 117609"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sami","family":"Bahri","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology, Singapore 117609"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1076","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Antonin, W., Riedel, D., and von Mollard, G.F. 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Identification and cDNA cloning of a novel human mosaic protein, LGN, based on interaction with G\u03b1i2.Gene181,39\u201343.","DOI":"10.1016\/S0378-1119(96)00456-8"},{"key":"REF20","doi-asserted-by":"crossref","unstructured":"Natochin, M., Lester, B., Peterson, Y.K., Bernard, M.L., Lanier, S.M., and Artemyev, N.O. (2000). AGS3 inhibits GDP dissociation from galpha subunits of the Gi family and rhodopsin-dependent activation of transducin.J. Biol. Chem.275,40981\u201340985.","DOI":"10.1074\/jbc.M006478200"},{"key":"REF21","doi-asserted-by":"crossref","unstructured":"Peterson, Y.K., Hazard, S., 3rd, Graber, S.G., and Lanier, S.M. (2000). Stabilization of the GDP-bound conformation of Gialpha by a peptide derived from the G-protein regulatory motif of AGS3.J. Biol. Chem.275,33193\u201333196.","DOI":"10.1074\/jbc.C000509200"},{"key":"REF22","doi-asserted-by":"crossref","unstructured":"Pizzinat, N., Takesono, A., and Lanier, S.M. (2001). Identification of a truncated form of the G-protein regulator AGS3 in heart that lacks the tetratricopeptide repeat domains.J. Biol. Chem.276,16601\u201316610.","DOI":"10.1074\/jbc.M007573200"},{"key":"REF23","doi-asserted-by":"crossref","unstructured":"Rosin-Arbesfeld, R., Ihruke, G., and Beinz, M. (2001). Actin-dependent membrane association of the APC tumour suppressor in polarized mammalian epithelial cells.EMBO J.20(21), 5929\u201339.","DOI":"10.1093\/emboj\/20.21.5929"},{"key":"REF24","doi-asserted-by":"crossref","unstructured":"Schaefer, M., Shevchenko, A., Shevchenko, A., and Knoblich, J.A. (2000). A protein complex containing Inscuteable and the Galpha-binding protein Pins orients asymmetric cell divisions in Drosophila.Curr. Biol.10,353\u2013362.","DOI":"10.1016\/S0960-9822(00)00401-2"},{"key":"REF25","doi-asserted-by":"crossref","unstructured":"Schaefer, M., Petronczki, M., Dorner, D., Forte, M., and Knoblich, J.A. (2001). Heterotrimeric G proteins direct two modes of asymmetric cell division in theDrosophilanervous system.Cell107,183\u2013194.","DOI":"10.1016\/S0092-8674(01)00521-9"},{"key":"REF26","doi-asserted-by":"crossref","unstructured":"Siderovski, D.P., Diverse-Pierluissi, M., and De Vries, L. (1999). The GoLoco motif: a Galphai\/o binding motif and potential guaninenucleotide exchange factor.Trends Biochem. Sci.24,340\u2013341.","DOI":"10.1016\/S0968-0004(99)01441-3"},{"key":"REF27","doi-asserted-by":"crossref","unstructured":"Stow, J.L., de Almeida, J.B., Narula, N., Holtzman, E.J., Ercolani, L., and Ausiello, D.A. (1991). A heterotrimeric G protein, G alpha i-3, on Golgi membranes regulates the secretion of a heparan sulfate proteoglycan in LLC-PK1 epithelial cells.J. Cell Biol.114,1113\u20131124.","DOI":"10.1083\/jcb.114.6.1113"},{"key":"REF28","doi-asserted-by":"crossref","unstructured":"Summerton, J. (1999). 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Analysis of partner of inscuteable, a novel player ofDrosophilaasymmetric divisions, reveals two distinct steps in inscuteable apical localization.Cell100,399\u2013409.","DOI":"10.1016\/S0092-8674(00)80676-5"},{"key":"REF32","doi-asserted-by":"crossref","unstructured":"Yu, F., Ong, C.T., Chia, W., and Yang, X. (2002). Membrane targeting and asymmetric localization of Drosophila partner of inscuteable are discrete steps controlled by distinct regions of the protein.Mol. Cell. Biol.22,4230\u201342340.","DOI":"10.1128\/MCB.22.12.4230-4240.2002"},{"key":"REF33","doi-asserted-by":"crossref","unstructured":"Yu, F., Morin, X., Kaushik, R., Bahri, S., Yang, X., and Chia, W. (2003). A mouse homologue ofDrosophila pinscan asymmetrically localize and substitute forpinsfunction inDrosophilaneuroblasts.J. Cell Sci.116,887\u2013896.","DOI":"10.1242\/jcs.00297"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2021,6,6]],"date-time":"2021-06-06T23:59:42Z","timestamp":1623023982000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.e03-04-0212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8]]},"references-count":33,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["10.1091\/mbc.e03-04-0212"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e03-04-0212","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8]]}}}