{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T17:11:02Z","timestamp":1764349862719},"reference-count":41,"publisher":"American Society for Cell Biology (ASCB)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p>Mcl-1 functions at an apical step in many regulatory programs that control cell death. Although the mitochondrion is one major subcellular organelle where Mcl-1 functions, the molecular mechanism by which Mcl-1 is targeted to mitochondria remains unclear. Here, we demonstrate that Mcl-1 is loosely associated with the outer membrane of mitochondria. Furthermore, we demonstrate that Mcl-1 interacts with the mitochondrial import receptor Tom70, and such interaction requires an internal domain of Mcl-1 that contains an EELD motif. A Tom70 antibody that blocks Mcl-1\u2013Tom70 interaction blocks mitochondrial import of Mcl-1 in vitro. Furthermore, Mcl-1 is significantly less targeted to mitochondria in Tom70 knockdown than in the control cells. Similar targeting preference is also observed for the DM mutant of Mcl-1 whose mutation at the EELD motif markedly attenuates its Tom70 binding activity. Together, our results indicate that the internal EELD domain facilitates mitochondrial targeting of Mcl-1 via a Tom70-dependent pathway.<\/jats:p>","DOI":"10.1091\/mbc.e06-04-0319","type":"journal-article","created":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T08:53:12Z","timestamp":1152175992000},"page":"3952-3963","source":"Crossref","is-referenced-by-count":36,"title":["An Internal EELD Domain Facilitates Mitochondrial Targeting of Mcl-1 via a Tom70-dependent Pathway"],"prefix":"10.1091","volume":"17","author":[{"given":"Chiang-Hung","family":"Chou","sequence":"first","affiliation":[{"name":"*Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan;"}]},{"given":"Ru-Shuo","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate Institute of Cell and Molecular Biology, Taipei Medical University, Taipei 110, Taiwan; and"}]},{"given":"Hsin-Fang","family":"Yang-Yen","sequence":"additional","affiliation":[{"name":"*Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan;"},{"name":"Graduate Institute of Cell and Molecular Biology, Taipei Medical University, Taipei 110, Taiwan; and"},{"name":"Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1046\/j.1471-4159.1999.0732240.x","volume":"73","author":"Alvarez-Dolado M.","year":"1999","journal-title":"J. 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