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Notably, palmitoylation had minimal influence on the density of tetraspanin protein complexes, did not promote tetraspanin localization into detergent-resistant microdomains, and was not required for CD151-\u03b13\u03b21 integrin association. However, the CD151 tetra mutant showed markedly diminished associations with other cell surface proteins, including other transmembrane 4 superfamily proteins (CD9, CD63). Thus, palmitoylation may be critical for assembly of the large network of cell surface tetraspanin-protein interactions, sometimes called the \u201ctetraspanin web.\u201d Also, compared with wild-type CD151, the tetra mutant was much more diffusely distributed and showed markedly diminished stability during biosynthesis. Finally, expression of the tetra-CD151 mutant profoundly altered \u03b13 integrin-deficient kidney epithelial cells, such that they converted from a dispersed, elongated morphology to an epithelium-like cobblestone clustering. These results point to novel biochemical and biological functions for tetraspanin palmitoylation.<\/jats:p>","DOI":"10.1091\/mbc.01-05-0275","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T22:19:36Z","timestamp":1027721976000},"page":"767-781","source":"Crossref","is-referenced-by-count":213,"title":["Palmitoylation of Tetraspanin Proteins: Modulation of CD151 Lateral Interactions, Subcellular Distribution, and Integrin-dependent Cell Morphology"],"prefix":"10.1091","volume":"13","author":[{"given":"Xiuwei","family":"Yang","sequence":"first","affiliation":[{"name":"Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Claas","sequence":"additional","affiliation":[{"name":"Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stine-Kathrein","family":"Kraeft","sequence":"additional","affiliation":[{"name":"Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan Bo","family":"Chen","sequence":"additional","affiliation":[{"name":"Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zemin","family":"Wang","sequence":"additional","affiliation":[{"name":"Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan A.","family":"Kreidberg","sequence":"additional","affiliation":[{"name":"Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin E.","family":"Hemler","sequence":"additional","affiliation":[{"name":"Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0022-1759(99)00102-7","volume":"229","author":"Azorsa D.O.","year":"1999","journal-title":"J. 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