{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:15:27Z","timestamp":1791130527264,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Cell Biology (ASCB)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:p>Transforming growth factor-\u03b2 (TGF-\u03b2) superfamily members regulate a wide range of biological processes by binding to two transmembrane serine\/threonine kinase receptors, type I and type II. We have previously shown that the internalization of these receptors is inhibited by K<jats:sup>+<\/jats:sup>depletion, cytosol acidification, or hypertonic medium, suggesting the involvement of clathrin-coated pits. However, the involvement of the clathrin-associated adaptor complex AP2 and the identity of the AP2 subunit that binds the receptors were not known. Herein, we have studied these issues by combining studies on intact cells with in vitro assays. Using fluorescence photobleaching recovery to measure the lateral mobility of the receptors on live cells (untreated or treated to alter their coated pit structure), we demonstrated that their mobility is restricted by interactions with coated pits. These interactions were transient and mediated through the receptors' cytoplasmic tails. To measure direct binding of the receptors to specific AP2 subunits, we used yeast two-hybrid screens and in vitro biochemical assays. In contrast to most other plasma membrane receptors that bind to AP2 via the \u03bc2 subunit, AP2\/TGF-\u03b2 receptor binding was mediated by a direct interaction between the \u03b22-adaptin N-terminal trunk domain and the cytoplasmic tails of the receptors; no binding was observed to the \u03bc2, \u03b1, or \u03c22 subunits of AP2 or to \u03bc1 of AP1. The data uniquely demonstrate both in vivo and in vitro the ability of \u03b22-adaptin to directly couple TGF-\u03b2 receptors to AP2 and to clathrin-coated pits, providing the first in vivo evidence for interactions of a transmembrane receptor with \u03b22-adaptin.<\/jats:p>","DOI":"10.1091\/mbc.02-07-0104","type":"journal-article","created":{"date-parts":[[2002,11,12]],"date-time":"2002-11-12T19:42:39Z","timestamp":1037130159000},"page":"4001-4012","source":"Crossref","is-referenced-by-count":119,"title":["Transforming Growth Factor-\u03b2 Receptors Interact with AP2 by Direct Binding to \u03b22 Subunit"],"prefix":"10.1091","volume":"13","author":[{"given":"Diying","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Thoracic Diseases Research Unit, Mayo Clinic, Rochester, Minnesota 55905; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Ehrlich","sequence":"additional","affiliation":[{"name":"Department of Neurobiochemistry, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoav I.","family":"Henis","sequence":"additional","affiliation":[{"name":"Department of Neurobiochemistry, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward B.","family":"Leof","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Thoracic Diseases Research Unit, Mayo Clinic, Rochester, Minnesota 55905; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.11.2133"},{"key":"B2","doi-asserted-by":"crossref","first-page":"23118","DOI":"10.1074\/jbc.273.36.23118","volume":"273","author":"Anders R.A.","year":"1998","journal-title":"J. Biol. Chem."},{"key":"B3","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1016\/S0006-3495(76)85755-4","volume":"16","author":"Axelrod D.","year":"1976","journal-title":"Biophys. J."},{"key":"B4","doi-asserted-by":"crossref","first-page":"12111","DOI":"10.1074\/jbc.271.20.12111","volume":"271","author":"Benmerah A.","year":"1996","journal-title":"J. Biol. Chem."},{"key":"B5","doi-asserted-by":"crossref","first-page":"5789","DOI":"10.1002\/j.1460-2075.1996.tb00965.x","volume":"15","author":"Boll W.","year":"1996","journal-title":"EMBO J."},{"key":"B6","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1083\/jcb.145.5.923","volume":"145","author":"Bonifacino J.S.","year":"1999","journal-title":"J. Cell Biol."},{"key":"B7","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/29555","volume":"394","author":"Chen H.","year":"1998","journal-title":"Nature"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.3.675"},{"key":"B9","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1242\/jcs.114.9.1777","volume":"114","author":"Ehrlich M.","year":"2001","journal-title":"J. Cell Sci."},{"key":"B10","doi-asserted-by":"crossref","first-page":"21075","DOI":"10.1074\/jbc.270.36.21075","volume":"270","author":"Fire E.","year":"1995","journal-title":"J. Biol. Chem."},{"key":"B11","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1083\/jcb.115.6.1585","volume":"115","author":"Fire E.","year":"1991","journal-title":"J. Cell Biol."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.3.1023"},{"key":"B13","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1083\/jcb.140.4.767","volume":"140","author":"Gilboa L.","year":"1998","journal-title":"J. Cell Biol."},{"key":"B14","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/S0960-9822(07)00518-0","volume":"8","author":"Greenberg M.","year":"1998","journal-title":"Curr. Biol."},{"key":"B15","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1083\/jcb.121.1.61","volume":"121","author":"Hansen S.H.","year":"1993","journal-title":"J. Cell Biol."},{"key":"B16","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/0167-4889(83)90082-4","volume":"762","author":"Henis Y.I.","year":"1983","journal-title":"Biochim. Biophys. Acta"},{"key":"B17","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1083\/jcb.126.1.139","volume":"126","author":"Henis Y.I.","year":"1994","journal-title":"J. Cell Biol."},{"key":"B18","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1083\/jcb.108.2.401","volume":"108","author":"Heuser J.","year":"1989","journal-title":"J. Cell Biol."},{"key":"B19","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0167-4889(98)00056-1","volume":"1404","author":"Hirst J.","year":"1998","journal-title":"Biochim. Biophys. Acta"},{"key":"B20","doi-asserted-by":"crossref","first-page":"36153","DOI":"10.1074\/jbc.274.51.36153","volume":"274","author":"Hofmann M.W.","year":"1999","journal-title":"J. Biol. Chem."},{"key":"B21","doi-asserted-by":"crossref","first-page":"28356","DOI":"10.1074\/jbc.M102235200","volume":"276","author":"Keren T.","year":"2001","journal-title":"J. Biol. Chem."},{"key":"B22","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1083\/jcb.142.1.59","volume":"142","author":"Kibbey R.G.","year":"1998","journal-title":"J. Cell Biol."},{"key":"B23","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1146\/annurev.cellbio.15.1.705","volume":"15","author":"Kirchhausen T.","year":"1999","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"B24","doi-asserted-by":"crossref","first-page":"6423","DOI":"10.1074\/jbc.272.10.6423","volume":"272","author":"Koli K.M.","year":"1997","journal-title":"J. Biol. Chem."},{"key":"B25","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1016\/S0006-3495(76)85776-1","volume":"16","author":"Koppel D.E.","year":"1976","journal-title":"Biophys. J."},{"key":"B26","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/S0962-8924(96)10057-X","volume":"7","author":"Marks M.S.","year":"1997","journal-title":"Trends Cell Biol."},{"key":"B27","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1101\/gad.14.6.627","volume":"14","author":"Massagu\u00e9 J.","year":"2000","journal-title":"Genes Dev."},{"key":"B28","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1146\/annurev.cellbio.12.1.575","volume":"12","author":"Mellman I.","year":"1996","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"B29","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1152\/physrev.1997.77.3.759","volume":"77","author":"Mukherjee S.","year":"1997","journal-title":"Physiol. Rev."},{"key":"B30","doi-asserted-by":"crossref","first-page":"6320","DOI":"10.1074\/jbc.270.11.6320","volume":"270","author":"Nesterov A.","year":"1995","journal-title":"J. Biol. Chem."},{"key":"B31","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1126\/science.7569928","volume":"269","author":"Ohno H.","year":"1995","journal-title":"Science"},{"key":"B32","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/S0092-8674(00)80791-6","volume":"97","author":"Owen D.J.","year":"1999","journal-title":"Cell"},{"key":"B33","doi-asserted-by":"crossref","first-page":"4216","DOI":"10.1093\/emboj\/19.16.4216","volume":"19","author":"Owen D.J.","year":"2000","journal-title":"EMBO J."},{"key":"B34","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1083\/jcb.131.3.619","volume":"131","author":"Page L.J.","year":"1995","journal-title":"J. Cell Biol."},{"key":"B36","doi-asserted-by":"crossref","first-page":"2148","DOI":"10.1093\/emboj\/17.8.2148","volume":"17","author":"Rapoport I.","year":"1998","journal-title":"EMBO J."},{"key":"B37","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1093\/emboj\/16.9.2240","volume":"16","author":"Rapoport I.","year":"1997","journal-title":"EMBO J."},{"key":"B38","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1146\/annurev.biochem.66.1.511","volume":"66","author":"Schmid S.L.","year":"1997","journal-title":"Annu. Rev. Biochem."},{"key":"B39","doi-asserted-by":"crossref","first-page":"31083","DOI":"10.1074\/jbc.270.52.31083","volume":"270","author":"Shih W.","year":"1995","journal-title":"J. Biol. Chem."},{"key":"B40","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1126\/science.8342026","volume":"261","author":"Sorkin A.","year":"1993","journal-title":"Science"},{"key":"B41","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1002\/bies.950150603","volume":"15","author":"Sorkin A.","year":"1993","journal-title":"Bioessays"},{"key":"B42","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/S0968-0004(99)01519-4","volume":"25","author":"ten Dijke P.","year":"2000","journal-title":"Trends Biochem. Sci."},{"key":"B43","doi-asserted-by":"crossref","first-page":"8907","DOI":"10.1073\/pnas.96.16.8907","volume":"96","author":"Traub L.M.","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"B44","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1083\/jcb.123.5.1107","volume":"123","author":"Wang L.H.","year":"1993","journal-title":"J. Cell Biol."},{"key":"B45","doi-asserted-by":"crossref","first-page":"17056","DOI":"10.1074\/jbc.273.27.17056","volume":"273","author":"Warren R.A.","year":"1998","journal-title":"J. Biol. Chem."},{"key":"B46","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0092-8674(00)81556-1","volume":"100","author":"Wrana J.L.","year":"2000","journal-title":"Cell"},{"key":"B47","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/370341a0","volume":"370","author":"Wrana J.L.","year":"1994","journal-title":"Nature"},{"key":"B48","doi-asserted-by":"crossref","first-page":"27237","DOI":"10.1074\/jbc.M100033200","volume":"276","author":"Zwaagstra J.C.","year":"2001","journal-title":"J. Biol. Chem."}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2024,1,7]],"date-time":"2024-01-07T22:34:19Z","timestamp":1704666859000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.02-07-0104"}},"subtitle":[],"editor":[{"given":"Carl-Henrik","family":"Heldin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2002,11]]},"references-count":47,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2002,11]]}},"alternative-id":["10.1091\/mbc.02-07-0104"],"URL":"https:\/\/doi.org\/10.1091\/mbc.02-07-0104","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11]]}}}