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Using dominant negative alleles of rad3, we have identified two sites N-terminal to the conserved kinase domain that are essential for Rad3p function. One of these sites is the putative leucine zipper, which is conserved in other phosphatidylinositol 3-kinase-related family members. The other is a novel motif, which may also mediate Rad3p protein\u2013protein interactions.<\/jats:p>","DOI":"10.1091\/mbc.10.10.3223","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T23:19:39Z","timestamp":1372807179000},"page":"3223-3238","source":"Crossref","is-referenced-by-count":14,"title":["Requirement of Sequences outside the Conserved Kinase Domain of Fission Yeast Rad3p for Checkpoint Control"],"prefix":"10.1091","volume":"10","author":[{"given":"Carolyn Riley","family":"Chapman","sequence":"first","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah Tyler","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antony M.","family":"Carr","sequence":"additional","affiliation":[{"name":"Medical Research Council Cell Mutation, Sussex University, Falmer, Brighton, BN1 9RR, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamar","family":"Enoch","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1002\/j.1460-2075.1992.tb05179.x","volume":"11","author":"Al-Khodairy F.","year":"1992","journal-title":"EMBO J."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.5.2.147"},{"key":"B3","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1126\/science.281.5383.1674","volume":"281","author":"Banin S.","year":"1998","journal-title":"Science"},{"key":"B4","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0092-8674(00)80086-0","volume":"86","author":"Barlow C.","year":"1996","journal-title":"Cell"},{"key":"B5","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0378-1119(93)90552-E","volume":"123","author":"Basi G.","year":"1993","journal-title":"Gene"},{"key":"B6","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1038\/387516a0","volume":"387","author":"Baskaran R.","year":"1997","journal-title":"Nature"},{"key":"B7","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1080\/09553009414550211","volume":"65","author":"Beamish H.","year":"1994","journal-title":"Int. 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