{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:40:26Z","timestamp":1791348026015,"version":"4.3.1"},"reference-count":75,"publisher":"American Society for Cell Biology (ASCB)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[1999,10]]},"abstract":"<jats:p>Yeast Las17 protein is homologous to the Wiskott\u2013Aldrich Syndrome protein, which is implicated in severe immunodeficiency. Las17p\/Bee1p has been shown to be important for actin patch assembly and actin polymerization. Here we show that Las17p interacts with the Arp2\/3 complex. LAS17 is an allele-specific multicopy suppressor of ARP2 and ARP3 mutations; overexpression restores both actin patch organization and endocytosis defects in ARP2 temperature-sensitive (ts) cells. Six of seven ARP2 ts mutants and at least oneARP3 ts mutant are synthetically lethal withlas17\u0394 ts confirming functional interaction with the Arp2\/3 complex. Further characterization of las17\u0394cells showed that receptor-mediated internalization of \u03b1 factor by the Ste2 receptor is severely defective. The polarity of normal bipolar bud site selection is lost. Las17-gfp remains localized in cortical patches in vivo independently of polymerized actin and is required for the polarized localization of Arp2\/3 as well as actin. Coimmunoprecipitation of Arp2p with Las17p indicates that Las17p interacts directly with the complex. Two hybrid results also suggest that Las17p interacts with actin, verprolin, Rvs167p and several other proteins including Src homology 3 (SH3) domain proteins, suggesting that Las17p may integrate signals from different regulatory cascades destined for the Arp2\/3p complex and the actin cytoskeleton.<\/jats:p>","DOI":"10.1091\/mbc.10.10.3521","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T23:19:39Z","timestamp":1372807179000},"page":"3521-3538","source":"Crossref","is-referenced-by-count":147,"title":["The<i>Saccharomyces cerevisiae<\/i>Homologue of Human Wiskott\u2013Aldrich Syndrome Protein Las17p Interacts with the Arp2\/3 Complex"],"prefix":"10.1091","volume":"10","author":[{"given":"Ammar","family":"Madania","sequence":"first","affiliation":[{"name":"M\u00e9canismes Mol\u00e9culaires de la Division Cellulaire et du D\u00e9veloppement, Unit\u00e9 Propre de Recherche 9005 du Centre National de la Recherche Scientifique, Institut de Biologie Mol\u00e9culaire et Cellulaire, F-67084 Strasbourg, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Dumoulin","sequence":"additional","affiliation":[{"name":"M\u00e9canismes Mol\u00e9culaires de la Division Cellulaire et du D\u00e9veloppement, Unit\u00e9 Propre de Recherche 9005 du Centre National de la Recherche Scientifique, Institut de Biologie Mol\u00e9culaire et Cellulaire, F-67084 Strasbourg, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Grava","sequence":"additional","affiliation":[{"name":"M\u00e9canismes Mol\u00e9culaires de la Division Cellulaire et du D\u00e9veloppement, Unit\u00e9 Propre de Recherche 9005 du Centre National de la Recherche Scientifique, Institut de Biologie Mol\u00e9culaire et Cellulaire, F-67084 Strasbourg, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Kitamoto","sequence":"additional","affiliation":[{"name":"M\u00e9canismes Mol\u00e9culaires de la Division Cellulaire et du D\u00e9veloppement, Unit\u00e9 Propre de Recherche 9005 du Centre National de la Recherche Scientifique, Institut de Biologie Mol\u00e9culaire et Cellulaire, F-67084 Strasbourg, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Sch\u00e4rer-Brodbeck","sequence":"additional","affiliation":[{"name":"Biozentrum, University of Basel, CH-4056 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Soulard","sequence":"additional","affiliation":[{"name":"M\u00e9canismes Mol\u00e9culaires de la Division Cellulaire et du D\u00e9veloppement, Unit\u00e9 Propre de Recherche 9005 du Centre National de la Recherche Scientifique, Institut de Biologie Mol\u00e9culaire et Cellulaire, F-67084 Strasbourg, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Violaine","family":"Moreau","sequence":"additional","affiliation":[{"name":"M\u00e9canismes Mol\u00e9culaires de la Division Cellulaire et du D\u00e9veloppement, Unit\u00e9 Propre de Recherche 9005 du Centre National de la Recherche Scientifique, Institut de Biologie Mol\u00e9culaire et Cellulaire, F-67084 Strasbourg, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Winsor","sequence":"additional","affiliation":[{"name":"M\u00e9canismes Mol\u00e9culaires de la Division Cellulaire et du D\u00e9veloppement, Unit\u00e9 Propre de Recherche 9005 du Centre National de la Recherche Scientifique, Institut de Biologie Mol\u00e9culaire et Cellulaire, F-67084 Strasbourg, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1038\/nsb0195-28","volume":"2","author":"Amberg D.C.","year":"1995","journal-title":"Nat. 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