{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:40:00Z","timestamp":1791891600626,"version":"4.3.4"},"reference-count":34,"publisher":"American Society for Cell Biology (ASCB)","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:p>Occludin is an integral membrane protein with four transmembrane domains that is exclusively localized at tight junction (TJ) strands. Here, we describe the generation and analysis of mice carrying a null mutation in the occludin gene. Occludin \u2212\/\u2212 mice were born with no gross phenotype in the expected Mendelian ratios, but they showed significant postnatal growth retardation. Occludin \u2212\/\u2212 males produced no litters with wild-type females, whereas occludin \u2212\/\u2212 females produced litters normally when mated with wild-type males but did not suckle them. In occludin \u2212\/\u2212 mice, TJs themselves did not appear to be affected morphologically, and the barrier function of intestinal epithelium was normal as far as examined electrophysiologically. However, histological abnormalities were found in several tissues, i.e., chronic inflammation and hyperplasia of the gastric epithelium, calcification in the brain, testicular atrophy, loss of cytoplasmic granules in striated duct cells of the salivary gland, and thinning of the compact bone. These phenotypes suggested that the functions of TJs as well as occludin are more complex than previously supposed.<\/jats:p>","DOI":"10.1091\/mbc.11.12.4131","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T19:44:51Z","timestamp":1372794291000},"page":"4131-4142","source":"Crossref","is-referenced-by-count":1080,"title":["Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands"],"prefix":"10.1091","volume":"11","author":[{"given":"Mitinori","family":"Saitou","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikio","family":"Furuse","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Sasaki","sequence":"additional","affiliation":[{"name":"KAN Research Institute Inc., Kyoto Research Park, Chudoji, Shimogyo-ku, Kyoto 600-8317, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg-Dieter","family":"Schulzke","sequence":"additional","affiliation":[{"name":"Medizinische Klinik I Gastroenterologie und Infektiologie and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Fromm","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Physiologie, Universit\u00e4tsklinikum Benjamin Franklin, Freie Universit\u00e4t Berlin, Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Takano","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Cancer Institute, Toshima-ku, Tokyo 170-8455, Japan; and"},{"name":"Department of Molecular Genetics, Tohoku University School of Medicine, Sendai 980-8575, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Noda","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Cancer Institute, Toshima-ku, Tokyo 170-8455, Japan; and"},{"name":"Department of Molecular Genetics, Tohoku University School of Medicine, Sendai 980-8575, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoichiro","family":"Tsukita","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","first-page":"G467","volume":"269","author":"Anderson J.M.","year":"1995","journal-title":"Am. 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