{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:37:40Z","timestamp":1791351460108,"version":"4.3.1"},"reference-count":64,"publisher":"American Society for Cell Biology (ASCB)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p>Fertilization occurs after the completion of the sperm acrosome reaction, a secretory event that is triggered during gamete adhesion. ZP3, an egg zona pellucida glycoprotein, produces a sustained increase of the internal Ca<jats:sup>2+<\/jats:sup>concentration in mouse sperm, leading to acrosome reactions. Here we show that the sustained Ca<jats:sup>2+<\/jats:sup>concentration increase is due to the persistent activation of a Ca<jats:sup>2+<\/jats:sup>influx mechanism during the late stages of ZP3 signal transduction. These cells also possess a Ca<jats:sup>2+<\/jats:sup>store depletion\u2013activated Ca<jats:sup>2+<\/jats:sup>entry pathway that is open after treatment with thapsigargin. Thapsigargin and ZP3 activate the same Ca<jats:sup>2+<\/jats:sup>permeation mechanism, as demonstrated by fluorescence quenching experiments and by channel antagonists. These studies show that ZP3 generates a sustained Ca<jats:sup>2+<\/jats:sup>influx through a store depletion\u2013operated pathway and that this drives the exocytotic acrosome reaction.<\/jats:p>","DOI":"10.1091\/mbc.11.5.1571","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T23:51:59Z","timestamp":1372809119000},"page":"1571-1584","source":"Crossref","is-referenced-by-count":189,"title":["Ca<sup>2+<\/sup>Entry through Store-operated Channels in Mouse Sperm Is Initiated by Egg ZP3 and Drives the Acrosome Reaction"],"prefix":"10.1091","volume":"11","author":[{"given":"Christine M.B.","family":"O'Toole","sequence":"first","affiliation":[{"name":"Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Arnoult","sequence":"additional","affiliation":[{"name":"Centre d'Etudes de Grenoble, Departement de Biologie Moleculaire et Structurale, 38054 Grenoble, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Darszon","sequence":"additional","affiliation":[{"name":"Department Gen\u00e8tica y Fisiolog\u0131\u0300a Molecular, Instituto d Biotecnologia, Cuernavaca, Morelos 62210, M\u00e8xico; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Steinhardt","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, California 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harvey M.","family":"Florman","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"13004","DOI":"10.1073\/pnas.93.23.13004","volume":"93","author":"Arnoult C.","year":"1996","journal-title":"Proc. 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Physiol."},{"key":"B11","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0143-4160(93)90018-2","volume":"14","author":"Blackmore P.F.","year":"1993","journal-title":"Cell Calcium"},{"key":"B12","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/0012-1606(83)90032-5","volume":"95","author":"Bleil J.D.","year":"1983","journal-title":"Dev. Biol."},{"key":"B13","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1083\/jcb.102.4.1363","volume":"102","author":"Bleil J.D.","year":"1986","journal-title":"J. Cell. Biol."},{"key":"B14","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/S0092-8674(00)80571-1","volume":"95","author":"Crabtree G.R.","year":"1999","journal-title":"Cell"},{"key":"B15","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1152\/physrev.1999.79.2.481","volume":"79","author":"Darszon A.","year":"1999","journal-title":"Physiol. Rev."},{"key":"B16","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1002\/(SICI)1520-6408(1999)25:2<103::AID-DVG4>3.0.CO;2-B","volume":"25","author":"Espinosa F.","year":"1999","journal-title":"Dev. Gen."},{"key":"B17","doi-asserted-by":"crossref","first-page":"9297","DOI":"10.1074\/jbc.273.15.9297","volume":"273","author":"Fagan K.A.","year":"1998","journal-title":"J. Biol. Chem."},{"key":"B18","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1083\/jcb.131.3.655","volume":"131","author":"Fanger C.M.","year":"1995","journal-title":"J. Cell Biol."},{"key":"B19","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1006\/dbio.1994.1242","volume":"165","author":"Florman H.M.","year":"1994","journal-title":"Dev. Biol."},{"key":"B20","first-page":"147","volume":"5","author":"Florman H.M.","year":"1999","journal-title":"Adv. Dev. Biochem."},{"key":"B21","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/0012-1606(92)90137-6","volume":"152","author":"Florman H.M.","year":"1992","journal-title":"Dev. Biol."},{"key":"B22","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0012-1606(89)90164-4","volume":"135","author":"Florman H.M.","year":"1989","journal-title":"Dev. Biol."},{"key":"B23","doi-asserted-by":"crossref","first-page":"3711","DOI":"10.1523\/JNEUROSCI.19-10-03711.1999","volume":"19","author":"Fomina A.F.","year":"1999","journal-title":"J. Neurosci."},{"key":"B24","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1002\/mrd.1080400213","volume":"40","author":"Fraser L.R.","year":"1995","journal-title":"Mol. Reprod. Dev."},{"key":"B25","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1006\/bbrc.1997.7458","volume":"239","author":"Garcia R.L.","year":"1997","journal-title":"Biochem. Biophys. Res. 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Chem."},{"key":"B36","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1002\/mrd.1120240307","volume":"24","author":"Lee M.A.","year":"1989","journal-title":"Gamete Res."},{"key":"B37","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0896-6273(00)80838-7","volume":"24","author":"Li H.-S.","year":"1999","journal-title":"Neuron"},{"key":"B38","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/0014-5793(96)00515-7","volume":"388","author":"Li\u00e9vano A.","year":"1996","journal-title":"FEBS Lett."},{"key":"B39","doi-asserted-by":"crossref","first-page":"5791","DOI":"10.1073\/pnas.96.10.5791","volume":"96","author":"Liman E.R.","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"B40","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1002\/jez.1402670312","volume":"267","author":"Meizel S.","year":"1993","journal-title":"J. Exp. 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