{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:10:36Z","timestamp":1791353436416,"version":"4.3.1"},"reference-count":42,"publisher":"American Society for Cell Biology (ASCB)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p>The tight junction is the most apical intercellular junction of epithelial cells and regulates transepithelial permeability through the paracellular pathway. To examine possible functions for the tight junction-associated protein ZO-1, C-terminally truncated mutants and a deletion mutant of ZO-1 were epitope tagged and stably expressed in corneal epithelial cell lines. Only full-length ZO-1 and one N-terminal truncation mutant targeted to cell borders; other mutants showed variable cytoplasmic distributions. None of the mutants initially disrupted the localization of endogenous ZO-1. However, long-term stable expression of two of the N-terminal mutants resulted in a dramatic change in cell shape and patterns of gene expression. An elongated fibroblast-like shape replaced characteristic epithelial cobblestone morphology. In addition, vimentin and smooth muscle actin expression were up-regulated, although variable cytokeratin expression remained, suggesting a partial transformation to a mesenchymal cell type. Concomitant with the morphological change, the expression of the integral membrane tight junction protein occludin was significantly down-regulated. The localizations of endogenous ZO-1 and another family member, ZO-2, were disrupted. These findings suggest that ZO-1 may participate in regulation of cellular differentiation.<\/jats:p>","DOI":"10.1091\/mbc.11.5.1687","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T23:51:59Z","timestamp":1372809119000},"page":"1687-1696","source":"Crossref","is-referenced-by-count":67,"title":["Truncation Mutants of the Tight Junction Protein ZO-1 Disrupt Corneal Epithelial Cell Morphology"],"prefix":"10.1091","volume":"11","author":[{"given":"Sandra W.","family":"Ryeom","sequence":"first","affiliation":[{"name":"Departments of Cell Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Paul","sequence":"additional","affiliation":[{"name":"Neurobiology, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Goodenough","sequence":"additional","affiliation":[{"name":"Departments of Cell Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"27489","DOI":"10.1074\/jbc.270.46.27489","volume":"270","author":"Alessi D.R.","year":"1995","journal-title":"J. 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