{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:22:45Z","timestamp":1778275365960,"version":"3.51.4"},"reference-count":39,"publisher":"American Society for Cell Biology (ASCB)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:p>Cyclic AMP (cAMP) stimulates the transport of Na<jats:sup>+<\/jats:sup>and Na,K-ATPase activity in the renal cortical collecting duct (CCD). The aim of this study was to investigate the mechanism whereby cAMP stimulates the Na,K-ATPase activity in microdissected rat CCDs and cultured mouse mpkCCD<jats:sub>c14<\/jats:sub>collecting duct cells. db-cAMP (10<jats:sup>\u22123<\/jats:sup>M) stimulated by 2-fold the activity of Na,K-ATPase from rat CCDs as well as the ouabain-sensitive component of<jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup>uptake by rat CCDs (1.7-fold) and cultured mouse CCD cells (1.5-fold). Pretreatment of rat CCDs with saponin increased the total Na,K-ATPase activity without further stimulation by db-cAMP. Western blotting performed after a biotinylation procedure revealed that db-cAMP increased the amount of Na,K-ATPase at the cell surface in both intact rat CCDs (1.7-fold) and cultured cells (1.3-fold), and that this increase was not related to changes in Na,K-ATPase internalization. Brefeldin A and low temperature (20\u00b0C) prevented both the db-cAMP-dependent increase in cell surface expression and activity of Na,K-ATPase in both intact rat CCDs and cultured cells. Pretreatment with the intracellular Ca<jats:sup>2+<\/jats:sup>chelator bis-(o-aminophenoxy)-N,N,N\u2032,N\u2032-tetraacetic acid also blunted the increment in cell surface expression and activity of Na,K-ATPase caused by db-cAMP. In conclusion, these results strongly suggest that the cAMP-dependent stimulation of Na,K-ATPase activity in CCD results from the translocation of active pump units from an intracellular compartment to the plasma membrane.<\/jats:p>","DOI":"10.1091\/mbc.12.2.255","type":"journal-article","created":{"date-parts":[[2013,7,3]],"date-time":"2013-07-03T00:08:09Z","timestamp":1372810089000},"page":"255-264","source":"Crossref","is-referenced-by-count":61,"title":["Cyclic AMP Increases Cell Surface Expression of Functional Na,K-ATPase Units in Mammalian Cortical Collecting Duct Principal Cells"],"prefix":"10.1091","volume":"12","author":[{"given":"Sandrine","family":"Gonin","sequence":"first","affiliation":[{"name":"Division de N\u00e9phrologie, Fondation pour Recherches M\u00e9dicales, CH-1211 Gen\u00e8ve 4, Switzerland;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georges","family":"Desch\u00eanes","sequence":"additional","affiliation":[{"name":"Service de N\u00e9phrologie P\u00e9diatrique, H\u00f4pital A. 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