{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T19:44:19Z","timestamp":1771011859082,"version":"3.50.1"},"reference-count":0,"publisher":"American Society for Cell Biology (ASCB)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Regul"],"published-print":{"date-parts":[[1991,4]]},"abstract":"<jats:p> Nuclear factor kappa B (NF-kappa B) is a ubiquitous transcription factor that affects expression of many genes, including immunoglobulin kappa (kappa), the interleukin-2 receptor alpha chain, and two genes in HIV-1. NF-kappa B can be activated by a number of stimuli, including pharmacological stimulation of protein kinase C by phorbol 12-myristate 13-acetate (PMA) and treatment in vitro with either protein kinase C or protein kinase A. This has lead to the proposal that these kinases are key enzymes in the physiological activation of NF-kappa B as well. We have used a murine B cell line, 70Z\/3, and T cell line, EL-4 6.1 C10, to study the activation of NF-kappa B by two physiological activators, interleukin-1 alpha (IL-1) and lipopolysaccharide (LPS). There are four reasons to propose that these agents activate pathways that do not include protein kinase C as a major component in these cell lines. First, the protein kinase C inhibitor 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7) strongly inhibited PMA-induced activation of NF-kappa B in 70Z\/3 cells but had no effect on NF-kappa B activated by IL-1 or LPS. Second, depletion of protein kinase C by prolonged growth of 70Z\/3 in PMA abrogated the capacity of the cells to activate NF-kappa B in response to further PMA treatment. However, these same cells activated NF-kappa B normally after either IL-1 or LPS treatment. Third, IL-1 effectively activated NF-kappa B in EL-4 6.1 C10 cells, but PMA did not. Fourth, interferon-gamma is a potent activator of protein kinase C in 70Z\/3 cells, but is completely inactive in the mobilization of NF-kappa B. These results suggest that the physiological inducers IL-1 and LPS activate NF-kappa B by pathways independent of protein kinase C in both 70Z\/3 and EL-4 6.1 C10 cells. <\/jats:p>","DOI":"10.1091\/mbc.2.4.329","type":"journal-article","created":{"date-parts":[[2013,8,16]],"date-time":"2013-08-16T18:45:02Z","timestamp":1376678702000},"page":"329-335","source":"Crossref","is-referenced-by-count":52,"title":["Evidence that interleukin-1 and phorbol esters activate NF-kappa B by different pathways: role of protein kinase C."],"prefix":"10.1091","volume":"2","author":[{"given":"K","family":"Bomsztyk","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle 98195, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J W","family":"Rooney","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle 98195, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle 98195, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N A","family":"Rachie","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle 98195, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S K","family":"Dower","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle 98195, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C H","family":"Sibley","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle 98195, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1076","container-title":["Cell Regulation"],"original-title":[],"language":"en","deposited":{"date-parts":[[2019,7,14]],"date-time":"2019-07-14T11:16:18Z","timestamp":1563102978000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.2.4.329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1991,4]]}},"alternative-id":["10.1091\/mbc.2.4.329"],"URL":"https:\/\/doi.org\/10.1091\/mbc.2.4.329","relation":{},"ISSN":["1044-2030"],"issn-type":[{"value":"1044-2030","type":"print"}],"subject":[],"published":{"date-parts":[[1991,4]]}}}