{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T11:40:20Z","timestamp":1773574820681,"version":"3.50.1"},"reference-count":0,"publisher":"American Society for Cell Biology (ASCB)","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[1995,12]]},"abstract":"<jats:p> Basic fibroblast growth factor (FGF-2) is a pleiotropic growth factor detected in many different cells and tissues. Normally synthesized at low levels, FGF-2 is elevated in various pathologies, most notably in cancer and injury repair. To investigate the effects of elevated FGF-2, the human full-length cDNA was expressed in transgenic mice under control of a phosphoglycerate kinase promoter. Overexpression of FGF-2 caused a variety of skeletal malformations including shortening and flattening of long bones and moderate macrocephaly. Comparison by Western blot of FGF-2 transgenic mice to nontransgenic littermates showed expression of human FGF-2 protein in all major organs and tissues examined including brain, heart, lung, liver, kidney, spleen, and skeletal muscle; however, different molar ratios of FGF-2 protein isoforms were observed between different organs and tissues. Some tissues preferentially synthesize larger isoforms of FGF-2 while other tissues produce predominantly smaller 18-kDa FGF-2. Translation of the high molecular weight isoforms initiates from unconventional CUG codons and translation of the 18-kDa isoform initiates from an AUG codon in the FGF-2 mRNA. Thus the Western blot data from the FGF-2 transgenic mice suggest that tissue-specific expression of FGF-2 isoforms is regulated translationally. <\/jats:p>","DOI":"10.1091\/mbc.6.12.1861","type":"journal-article","created":{"date-parts":[[2013,8,16]],"date-time":"2013-08-16T19:07:19Z","timestamp":1376680039000},"page":"1861-1873","source":"Crossref","is-referenced-by-count":249,"title":["Abnormal bone growth and selective translational regulation in basic fibroblast growth factor (FGF-2) transgenic mice."],"prefix":"10.1091","volume":"6","author":[{"given":"J D","family":"Coffin","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92121, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R Z","family":"Florkiewicz","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92121, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Neumann","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92121, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Mort-Hopkins","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92121, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"2nd","given":"G W","family":"Dorn","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92121, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Lightfoot","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92121, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"German","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92121, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P N","family":"Howles","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92121, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Kier","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92121, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B A","family":"O'Toole","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92121, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1076","container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2019,7,14]],"date-time":"2019-07-14T13:48:10Z","timestamp":1563112090000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.6.12.1861"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1995,12]]}},"alternative-id":["10.1091\/mbc.6.12.1861"],"URL":"https:\/\/doi.org\/10.1091\/mbc.6.12.1861","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,12]]}}}