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When exocytosis is stimulated with \u03b1-latrotoxin, VAMP2 dissociates from synaptophysin I even in the absence of appreciable exocytosis, whereas synaptophysin I oligomers disassemble only upon incorporation of the vesicle with the plasma membrane. We propose that synaptophysin I has multiple roles in neurotransmitter release, regulating VAMP2 availability for the solubleN-ethylmaleimide-sensitive factor attachment protein receptor complex and possibly participating in the late steps of exocytosis.<\/jats:p>","DOI":"10.1091\/mbc.e02-01-0036","type":"journal-article","created":{"date-parts":[[2002,9,30]],"date-time":"2002-09-30T17:42:46Z","timestamp":1033407766000},"page":"2706-2717","source":"Crossref","is-referenced-by-count":50,"title":["Fluorescence Resonance Energy Transfer Detection of Synaptophysin I and Vesicle-associated Membrane Protein 2 Interactions during Exocytosis from Single Live Synapses"],"prefix":"10.1091","volume":"13","author":[{"given":"Maria","family":"Pennuto","sequence":"first","affiliation":[{"name":"Department of Neuroscience, S. 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