{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:55:37Z","timestamp":1790866537808,"version":"4.1.0"},"reference-count":55,"publisher":"American Society for Cell Biology (ASCB)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:p>The survival of Trypanosoma brucei, the causative agent of Sleeping Sickness and Nagana, is facilitated by the expression of a dense surface coat of glycosylphosphatidylinositol (GPI)-anchored proteins in both its mammalian and tsetse fly hosts. We have characterized T. brucei GPI8, the gene encoding the catalytic subunit of the GPI:protein transamidase complex that adds preformed GPI anchors onto nascent polypeptides. Deletion ofGPI8 (to give \u0394gpi8) resulted in the absence of GPI-anchored proteins from the cell surface of procyclic form trypanosomes and accumulation of a pool of non\u2013protein-linked GPI molecules, some of which are surface located. Procyclic \u0394gpi8, while viable in culture, were unable to establish infections in the tsetse midgut, confirming that GPI-anchored proteins are essential for insect-parasite interactions. Applying specific inducible GPI8 RNAi with bloodstream form parasites resulted in accumulation of unanchored variant surface glycoprotein and cell death with a defined multinuclear, multikinetoplast, and multiflagellar phenotype indicative of a block in cytokinesis. These data show that GPI-anchored proteins are essential for the viability of bloodstream form trypanosomes even in the absence of immune challenge and imply that GPI8 is important for proper cell cycle progression.<\/jats:p>","DOI":"10.1091\/mbc.e02-03-0167","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T22:44:48Z","timestamp":1047422688000},"page":"1182-1194","source":"Crossref","is-referenced-by-count":100,"title":["Essential Roles for GPI-anchored Proteins in African Trypanosomes Revealed Using Mutants Deficient in GPI8"],"prefix":"10.1091","volume":"14","author":[{"given":"Simon","family":"Lillico","sequence":"first","affiliation":[{"name":"Wellcome Centre for Molecular Parasitology, University of Glasgow, The Anderson College, Glasgow G11 6NU, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark C.","family":"Field","sequence":"additional","affiliation":[{"name":"Wellcome Trust Laboratories for Molecular Parasitology, Department of Biological Sciences and Centre for Molecular Microbiology and Infection, Imperial College, London SW7 2AY, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pat","family":"Blundell","sequence":"additional","affiliation":[{"name":"Wellcome Centre for Molecular Parasitology, University of Glasgow, The Anderson College, Glasgow G11 6NU, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham H.","family":"Coombs","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy C.","family":"Mottram","sequence":"additional","affiliation":[{"name":"Wellcome Centre for Molecular Parasitology, University of Glasgow, The Anderson College, Glasgow G11 6NU, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1006\/jmbi.2000.4246","volume":"304","author":"Acosta-Serrano A.","year":"2000","journal-title":"J. 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