{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:24:52Z","timestamp":1791296692405,"version":"4.1.0"},"reference-count":56,"publisher":"American Society for Cell Biology (ASCB)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:p>The bullous pemphigoid antigen 1 (BP230) and desmoplakin (DP) are members of the plakin protein family of cytolinkers. Despite their homology, their COOH termini selectively bind distinct intermediate filaments (IFs). We studied sequences within their COOH termini required for their interaction with the epidermal keratins K5\/K14, the simple epithelial keratins K8\/K18, and type III IF vimentin by yeast three-hybrid, cell transfection, and overlay assays. The results indicate that BP230 interacts with K5\/K14 but not with K8\/K18 or vimentin via a region encompassing both the B and C subdomains and the COOH extremity, including a COOH-terminal eight-amino-acid stretch. In contrast, the C subdomain with the COOH-terminal extremity of DP interacts with K5\/K14 and K8\/K18, and its linker region is able to associate with K8\/K18 and vimentin. Furthermore, the potential of DP to interact with IF proteins in yeast seems to be regulated by phosphorylation of Ser 2849 within its COOH terminus. Strikingly, BP230 and DP interacted with cytokeratins only when both type I and type II keratins were present. The head and tail domains of K5\/K14 keratins were dispensable for their interaction with BP230 or DP. On the basis of our findings, we postulate that (1) the binding specificity of plakins for various IF proteins depends on their linker region between the highly homologous B and C subdomains and their COOH extremity and (2) the association of DP and BP230 with both epidermal and simple keratins is critically affected by the tertiary structure induced by heterodimerization and involves recognition sites located primarily in the rod domain of these keratins.<\/jats:p>","DOI":"10.1091\/mbc.e02-08-0548","type":"journal-article","created":{"date-parts":[[2003,5,28]],"date-time":"2003-05-28T04:45:36Z","timestamp":1054097136000},"page":"1978-1992","source":"Crossref","is-referenced-by-count":99,"title":["Interaction of the Bullous Pemphigoid Antigen 1 (BP230) and Desmoplakin with Intermediate Filaments Is Mediated by Distinct Sequences within Their COOH Terminus"],"prefix":"10.1091","volume":"14","author":[{"given":"Lionel","family":"Fontao","sequence":"first","affiliation":[{"name":"Department of Dermatology, University Hospital, Geneva, Switzerland CH-1211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Favre","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital, Geneva, Switzerland CH-1211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Riou","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital, Geneva, Switzerland CH-1211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Geerts","sequence":"additional","affiliation":[{"name":"Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Jaunin","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital, Geneva, Switzerland CH-1211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Hilaire","family":"Saurat","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital, Geneva, Switzerland CH-1211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen J.","family":"Green","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Dermatology and the R.H. Lurie Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnoud","family":"Sonnenberg","sequence":"additional","affiliation":[{"name":"Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Borradori","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital, Geneva, Switzerland CH-1211"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Andr\u00e4, K., Lassmann, H., Bittner, R., Shorny, S., F\u00e4ssler, R., Propst, F., and Wiche, G. (1997). 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Intermediate filaments and their associates: multi-talented structural elements specifying cytoarchitecture and cytodynamics.Curr. Opin. Cell Biol.12, 79\u201390.","DOI":"10.1016\/S0955-0674(99)00060-5"},{"key":"REF23","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.1.277"},{"key":"REF24","doi-asserted-by":"crossref","unstructured":"James, P., Halladay, J., and Craig, E.A. (1996). Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast.Genetics144, 1425\u20131436.","DOI":"10.1093\/genetics\/144.4.1425"},{"key":"REF25","doi-asserted-by":"crossref","unstructured":"Janda, L., Damborsky, J., Rezniczek, G., and Wiche, G. (2001). Plectin repeats, and modules: strategic cysteines and their presumed impact on cytolinker functions.Bioassays23, 1064\u20131069.","DOI":"10.1002\/bies.1151"},{"key":"REF26","doi-asserted-by":"crossref","unstructured":"Karashima, T., and Watt, F.M. (2002). Interaction of periplakin and envoplakin with intermediate filaments.J. Cell Sci.115, 5027\u20135037.","DOI":"10.1242\/jcs.00191"},{"key":"REF27","doi-asserted-by":"crossref","unstructured":"Kartenbeck, J., Franke, W.W., Moser, J.G., and Stoffels, U. (1983). Specific attachment of desmin filaments to desmosomal plaques in cardiac myocytes.EMBO J.2, 735\u2013742.","DOI":"10.1002\/j.1460-2075.1983.tb01493.x"},{"key":"REF28","doi-asserted-by":"crossref","unstructured":"Kartenbeck, J., Schwechheimer, K., Moll, R., and Franke, W.W. (1984). Attachment of vimentin filaments to desmosomal plaques in human meningiomal cells and arachnoidal tissue.J. Cell Biol.98, 1072\u20131081.","DOI":"10.1083\/jcb.98.3.1072"},{"key":"REF29","doi-asserted-by":"crossref","unstructured":"Kazerounian, S., Uitto, J., and Aho, S. (2002). Unique role for the periplakin tail in intermediate filament association: specific binding to keratin 8 and vimentin.Exp. Dermatol.11, 428\u2013438.","DOI":"10.1034\/j.1600-0625.2002.110506.x"},{"key":"REF30","doi-asserted-by":"crossref","unstructured":"Kouklis, P.D., Hutton, E., and Fuchs, E. (1994). Making a connection: direct binding between keratin intermediate filaments and desmosomal proteins.J. Cell Biol.127, 1049\u20131060.","DOI":"10.1083\/jcb.127.4.1049"},{"key":"REF31","doi-asserted-by":"crossref","unstructured":"Kowalczyk, A.P., Bornslaeger, E.A., Borgwardt, J.E., Palka, H.L., Dhaliwal, A.S., Corcoran, C.M., Denning, M.F., and Green, K.J. (1997). The amino-terminal domain of desmoplakin binds to plakoglobin and clusters desmosomal cadherin-plakoglobin complexes.J. Cell Biol.139, 773\u2013784.","DOI":"10.1083\/jcb.139.3.773"},{"key":"REF32","doi-asserted-by":"crossref","unstructured":"Leung, C.L., Green, K.J., and Liem, R.K. (2002). Plakins: a family of versatile cytolinker proteins.Trends Cell Biol.12, 37\u201345.","DOI":"10.1016\/S0962-8924(01)02180-8"},{"key":"REF33","doi-asserted-by":"crossref","unstructured":"Leung, C.L., and Liem, R.K. (1996). Characterization of interactions between the neurofilament triplet proteins by the yeast two-hybrid system.J. Biol. Chem.271, 14041\u201314044.","DOI":"10.1074\/jbc.271.24.14041"},{"key":"REF34","doi-asserted-by":"crossref","unstructured":"Leung, C.L., Liem, R.K., Parry, D.A., Green, K.J. (2001b). The plakin family.J. Cell Sci.114, 3409\u20133410.","DOI":"10.1242\/jcs.114.19.3409"},{"key":"REF35","doi-asserted-by":"crossref","unstructured":"Leung, C.L., Sun, D., and Liem, R.K. (1999). The intermediate filament protein peripherin is the specific interaction partner of mouse BPAG1-n (dystonin) in neurons.J. 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Intermediate filaments: molecular architecture, assembly, dynamics and polymorphism.Q. Rev. Biophys.32, 99\u2013187.","DOI":"10.1017\/S0033583500003516"},{"key":"REF42","doi-asserted-by":"crossref","unstructured":"Ruhrberg, C., and Watt, F.M. (1997). The plakin family: versatile organizers of cytoskeletal architecture.Curr. Opin. Genet. Dev.7, 392\u2013397.","DOI":"10.1016\/S0959-437X(97)80154-2"},{"key":"REF43","doi-asserted-by":"crossref","unstructured":"Schnabel, J., Weber, K., and Hatzfeld, M. (1998). Protein-protein interactions between keratin polypeptides expressed in the yeast two-hybrid system.Biochim. Biophys. Acta1403, 158\u2013168.","DOI":"10.1016\/S0167-4889(98)00036-6"},{"key":"REF44","doi-asserted-by":"crossref","unstructured":"Skaria, M., Jaunin, F., Hunziker, T., Riou, S., Schumann, H., Bruckner-Tuderman, L., Hertl, M., Bernard, P., Saurat, J.H., Favre, B., and Borradori, L. (2000). IgG autoantibodies from bullous pemphigoid patients recognize multiple antigenic reactive sites located predominantly within the B and C subdomains of the COOH-terminus of BP230.J. Invest. Dermatol.114, 998\u20131004.","DOI":"10.1046\/j.1523-1747.2000.00893.x"},{"key":"REF45","doi-asserted-by":"crossref","unstructured":"Smith, E.A., and Fuchs, E. (1998). Defining the interactions between intermediate filaments and desmosomes.J. Cell Biol.141, 1229\u20131241.","DOI":"10.1083\/jcb.141.5.1229"},{"key":"REF46","doi-asserted-by":"crossref","unstructured":"Stappenbeck, T.S., Bornslaeger, E.A., Corcoran, C.M., Luu, H.H., Virata, M.L., and Green, K.J. (1993). Functional analysis of desmoplakin domains: specification of the interaction with keratin versus vimentin intermediate filament networks.J. Cell Biol.123, 691\u2013705.","DOI":"10.1083\/jcb.123.3.691"},{"key":"REF47","doi-asserted-by":"crossref","unstructured":"Stappenbeck, T.S., Lamb, J.A., Corcoran, C.M., and Green, K.J. (1994). 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