{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:20:16Z","timestamp":1790832016997,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Cell Biology (ASCB)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:p>Of the &gt;20 epithelial keratins, keratin 20 (K20) has an unusual distribution and is poorly studied. We began to address K20 function, by expressing human wild-type and Arg80\u2192His (R80H) genomic (18 kb) and cDNA K20 in cells and mice. Arg80 of K20 is conserved in most keratins, and its mutation in epidermal keratins causes several skin diseases. R80H but not wild-type K20 generates disrupted keratin filaments in transfected cells. Transgenic mice that overexpress K20 R80H have collapsed filaments in small intestinal villus regions, when expressed at moderate levels, whereas wild-type K20-overexpressing mice have normal keratin networks. Overexpressed K20 maintains its normal distribution in several tissues, but not in the pancreas and stomach, without causing any tissue abnormalities. Hence, K20 pancreatic and gastric expression is regulated outside the 18-kb region. Cross-breeding of wild-type or R80H K20 mice with mice that overexpress wild-type K18 or K18 that is mutated at the conserved K20 Arg80-equivalent residue show that K20 plays an additive and compensatory role with K18 in maintaining keratin filament organization in the intestine. Our data suggest the presence of unique regulatory domains for pancreatic and gastric K20 expression and support a significant role for K20 in maintaining keratin filaments in intestinal epithelia.<\/jats:p>","DOI":"10.1091\/mbc.e03-02-0059","type":"journal-article","created":{"date-parts":[[2003,4,7]],"date-time":"2003-04-07T22:17:26Z","timestamp":1049753846000},"page":"2959-2971","source":"Crossref","is-referenced-by-count":78,"title":["Keratin 20 Helps Maintain Intermediate Filament Organization in Intestinal Epithelia"],"prefix":"10.1091","volume":"14","author":[{"given":"Qin","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Medicine, VA Palo Alto Health Care System, Palo Alto, California 94304, and the Digestive Disease Center, Stanford University School of Medicine, Palo Alto, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana M.","family":"Toivola","sequence":"additional","affiliation":[{"name":"Department of Medicine, VA Palo Alto Health Care System, Palo Alto, California 94304, and the Digestive Disease Center, Stanford University School of Medicine, Palo Alto, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningguo","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Medicine, VA Palo Alto Health Care System, Palo Alto, California 94304, and the Digestive Disease Center, Stanford University School of Medicine, Palo Alto, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry B.","family":"Greenberg","sequence":"additional","affiliation":[{"name":"Department of Medicine, VA Palo Alto Health Care System, Palo Alto, California 94304, and the Digestive Disease Center, Stanford University School of Medicine, Palo Alto, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner W.","family":"Franke","sequence":"additional","affiliation":[{"name":"German Cancer Research Center, Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Bishr","family":"Omary","sequence":"additional","affiliation":[{"name":"Department of Medicine, VA Palo Alto Health Care System, Palo Alto, California 94304, and the Digestive Disease Center, Stanford University School of Medicine, Palo Alto, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Abe, M., and Oshima, R.G. (1990). A single human keratin 18 gene is expressed in diverse epithelial cells of transgenic mice.\n                      J. Cell Biol.\n                      111\n                      , 1197-1206.","DOI":"10.1083\/jcb.111.3.1197"},{"key":"REF2","doi-asserted-by":"crossref","unstructured":"Anastasi, E., Ponte, E., Gradini, R., Bulotta, A., Sale, P., Tiberti, C., Okamoto, H., Dotta, F., and Mario, U.D. (1999). Expression of Reg and cytokeratin 20 during ductal cell differentiation and proliferation in a mouse model of autoimmune diabetes.\n                      Eur. J. 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The cytoskeleton of digestive epithelia in health and disease.\n                      Am. J. Physiol.\n                      277\n                      , G1108-G1137.","DOI":"10.1152\/ajpgi.1999.277.6.G1108"},{"key":"REF23","doi-asserted-by":"crossref","unstructured":"Leech, S.N., Kolar, A.J., Barrett, P.D., Sinclair, S.A., and Leonard, N. (2001). Merkel cell carcinoma can be distinguished from metastatic small cell carcinoma using antibodies to cytokeratin 20 and thyroid transcription factor 1.\n                      J. Clin. Pathol.\n                      54\n                      , 727-729.","DOI":"10.1136\/jcp.54.9.727"},{"key":"REF24","doi-asserted-by":"crossref","unstructured":"Magin, T.M., Hesse, M., and Schroder, R. (2000). 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Protein phosphatase inhibition in normal and keratin 8\/18 assembly-incompetent mouse strains supports a functional role of keratin intermediate filaments in preserving hepatocyte integrity.\n                      Hepatology\n                      28\n                      , 116-128.","DOI":"10.1002\/hep.510280117"},{"key":"REF35","doi-asserted-by":"crossref","unstructured":"Toivola, D.M., Ku, N.O., Ghori, N., Lowe, A.W., Michie, S.A., and Omary, M.B. (2000a). Effects of keratin filament disruption on exocrine pancreas-stimulated secretion and susceptibility to injury.\n                      Exp. Cell Res.\n                      255\n                      , 156-170.","DOI":"10.1006\/excr.1999.4787"},{"key":"REF36","doi-asserted-by":"crossref","unstructured":"Toivola, D.M., Baribault, H., Magin, T.M., Michie, S.A., and Omary, M.B. (2000b). Simple epithelial keratins are dispensable for cytoprotection in two pancreatitis models.\n                      Am. J. 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Cancer Res.\n                      10\n                      , 2840-2847."}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2021,6,6]],"date-time":"2021-06-06T01:45:25Z","timestamp":1622943925000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.e03-02-0059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":37,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1091\/mbc.e03-02-0059"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e03-02-0059","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1015001.195455","asserted-by":"object"}]},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]}}}