{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:37:25Z","timestamp":1791243445214,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Cell Biology (ASCB)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:p>In this article, we investigate the contributions of actin filaments and accessory proteins to apical clathrin-mediated endocytosis in primary rabbit lacrimal acini. Confocal fluorescence and electron microscopy revealed that cytochalasin D promoted apical accumulation of clathrin, \u03b1-adaptin, dynamin, and F-actin and increased the amounts of coated pits and vesicles at the apical plasma membrane. Sorbitol density gradient analysis of membrane compartments showed that cytochalasin D increased [<jats:sup>14<\/jats:sup>C]dextran association with apical membranes from stimulated acini, consistent with functional inhibition of apical endocytosis. Recombinant syndapin SH3 domains interacted with lacrimal acinar dynamin, neuronal Wiskott-Aldrich Syndrome protein (N-WASP), and synaptojanin; their introduction by electroporation elicited remarkable accumulation of clathrin, accessory proteins, and coated pits at the apical plasma membrane. These SH3 domains also significantly (p \u2264 0.05) increased F-actin, with substantial colocalization of dynamin and N-WASP with the additional filaments. Coelectroporation with the VCA domain of N-WASP blocked the increase in F-actin and reversed the morphological changes indicative of impaired apical endocytosis. We suggest that transient modulation of actin polymerization by syndapins through activation of the Arp2\/3 complex via N-WASP coordinates dynamin-mediated vesicle fission at the apical plasma membrane of acinar epithelia. Trapping of assembled F-actin intermediates during this process by cytochalasin D or syndapin SH3 domains impairs endocytosis.<\/jats:p>","DOI":"10.1091\/mbc.e03-05-0315","type":"journal-article","created":{"date-parts":[[2003,8,26]],"date-time":"2003-08-26T00:49:11Z","timestamp":1061858951000},"page":"4397-4413","source":"Crossref","is-referenced-by-count":46,"title":["Impairing Actin Filament or Syndapin Functions Promotes Accumulation of Clathrin-coated Vesicles at the Apical Plasma Membrane of Acinar Epithelial Cells"],"prefix":"10.1091","volume":"14","author":[{"given":"Silvia R.","family":"da Costa","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Southern California, Los Angeles, California 90033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunbyul","family":"Sou","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Southern California, Los Angeles, California 90033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiansong","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Southern California, Los Angeles, California 90033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francie A.","family":"Yarber","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Southern California, Los Angeles, California 90033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Curtis T.","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Southern California, Los Angeles, California 90033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Pidgeon","sequence":"additional","affiliation":[{"name":"Department of Cell and Neurobiology, University of Southern California, Los Angeles, California 90033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael M.","family":"Kessels","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Neurobiology, Magdeburg, Germany D-39118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin K.","family":"Mircheff","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Southern California, Los Angeles, California 90033"},{"name":"Department of Ophthalmology, University of Southern California, Los Angeles, California 90033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel E.","family":"Schechter","sequence":"additional","affiliation":[{"name":"Department of Cell and Neurobiology, University of Southern California, Los Angeles, California 90033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britta","family":"Qualmann","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Neurobiology, Magdeburg, Germany D-39118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah F.","family":"Hamm-Alvarez","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Southern California, Los Angeles, California 90033"},{"name":"Department of Physiology and Biophysics, University of Southern California, Los Angeles, California 90033"},{"name":"Department of Ophthalmology, University of Southern California, Los Angeles, California 90033"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Apodaca, G. 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