{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:36:10Z","timestamp":1791272170627,"version":"4.1.0"},"reference-count":64,"publisher":"American Society for Cell Biology (ASCB)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p>We report that the fission yeast nucleoporin Nup124p is required for the nuclear import of both, retrotransposon Tf1-Gag as well as the retroviral HIV-1 Vpr. Failure to import Tf1-Gag into the nucleus in a nup124 null mutant resulted in complete loss of Tf1 transposition. Similarly, nuclear import of HIV-1 Vpr was impaired in nup124 null mutant strains and cells became resistant to Vpr's cell-killing activity. On the basis of protein domain similarity, the human nucleoporin Nup153 was identified as a putative homolog of Nup124p. We demonstrate that in vitro\u2013translated Nup124p and Nup153 coimmunoprecipitate Tf1-Gag or HIV-1 Vpr. Though full-length Nup153 was unable to complement the Tf1 transposition defect in a nup124 null mutant, we provide evidence that both nucleoporins share a unique N-terminal domain, Nup124p<jats:sup>AA264\u2013454<\/jats:sup>and Nup153<jats:sup>AA448\u2013634<\/jats:sup>that is absolutely essential for Tf1 transposition. Epigenetic overexpression of this domain in a wild-type (nup124<jats:sup>+<\/jats:sup>) background blocked Tf1 activity implying that sequences from Nup124p and the human Nup153 challenged the same pathway affecting Tf1 transposition. Our results establish a unique relationship between two analogous nucleoporins Nup124p and Nup153 wherein the function of a common domain in retrotransposition is conserved.<\/jats:p>","DOI":"10.1091\/mbc.e04-07-0583","type":"journal-article","created":{"date-parts":[[2005,1,20]],"date-time":"2005-01-20T01:33:30Z","timestamp":1106184810000},"page":"1823-1838","source":"Crossref","is-referenced-by-count":42,"title":["The Functionally Conserved Nucleoporins Nup124p from Fission Yeast and the Human Nup153 Mediate Nuclear Import and Activity of the Tf1 Retrotransposon and HIV-1 Vpr"],"prefix":"10.1091","volume":"16","author":[{"given":"Padmapriya","family":"Varadarajan","sequence":"first","affiliation":[{"name":"Laboratory of Nucleopore Biology, Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sundarasamy","family":"Mahalingam","sequence":"additional","affiliation":[{"name":"Laboratory of Nucleopore Biology, Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore"},{"name":"Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Nucleopore Biology, Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah Boon Hsi","family":"Ng","sequence":"additional","affiliation":[{"name":"Laboratory of Nucleopore Biology, Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheetal","family":"Gandotra","sequence":"additional","affiliation":[{"name":"Laboratory of Nucleopore Biology, Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desmond Suresh Kumar","family":"Dorairajoo","sequence":"additional","affiliation":[{"name":"Laboratory of Nucleopore Biology, Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Balasundaram","sequence":"additional","affiliation":[{"name":"Laboratory of Nucleopore Biology, Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Atwood, A., Lin, J. H., and Levin, H. L. (1996). The retrotransposon Tf1 assembles virus-like particles that contain excess Gag relative to integrase because of a regulated degradation process.Mol. Cell. Biol.16, 338\u2013346.","DOI":"10.1128\/MCB.16.1.338"},{"key":"REF2","doi-asserted-by":"crossref","unstructured":"Balasundaram, D., Benedik, M. J., Morphew, M., Dang, V. D., and Levin, H. L. (1999). Nup124p is a nuclear pore factor ofSchizosaccharomyces pombethat is important for nuclear import and activity of retrotransposon Tf1.Mol. Cell. Biol.19, 5768\u20135784.","DOI":"10.1128\/MCB.19.8.5768"},{"key":"REF3","doi-asserted-by":"crossref","unstructured":"Ball, J. R., Dimaano, C., and Ullman, K .S. (2004). The RNA binding domain within the nucleoporin Nup153 associates preferentially with single-stranded RNA.RNA10, 19\u201327.","DOI":"10.1261\/rna.5109104"},{"key":"REF4","doi-asserted-by":"crossref","unstructured":"Bastos, R., Lin, A., Enarson, M., and Burke, B. (1996). 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Domain-specific antibodies reveal multiple-site topology of Nup153 within the nuclear pore complex.J. Struct. Biol.140, 254\u2013267.","DOI":"10.1016\/S1047-8477(02)00524-5"},{"key":"REF21","doi-asserted-by":"crossref","unstructured":"Fornerod, M., van Deursen, J., van Baal, S., Reynolds, A., Davis, D., Murti, K. G., Fransen, J., and Grosveld, G. (1997). The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN\/Nup214 and a novel nuclear pore component Nup88.EMBO J.16, 807\u2013816.","DOI":"10.1093\/emboj\/16.4.807"},{"key":"REF22","doi-asserted-by":"crossref","unstructured":"Fouchier, R. A., and Malim, M. H. (1999). Nuclear import of human immunodeficiency virus type-1 preintegration complexes.Adv. Virus Res.52, 275\u2013299.","DOI":"10.1016\/S0065-3527(08)60302-4"},{"key":"REF23","doi-asserted-by":"crossref","unstructured":"Fouchier, R. A., Meyer, B. E., Simon, J. H., Fischer, U., Albright, A. V., Gonzalez-Scarano, F., and Malim, M. H. (1998). Interaction of the human immunodeficiency virus type 1 Vpr protein with the nuclear pore complex.J. Virol.72, 6004\u20136013.","DOI":"10.1128\/JVI.72.7.6004-6013.1998"},{"key":"REF24","doi-asserted-by":"crossref","unstructured":"Griffis, E. R., Craige, B., Dimaano, C., Ullman, K. S., and Powers, M. A. (2004). Distinct functional domains within nucleoporins Nup153 and Nup98 mediate transcription dependent mobility.Mol. Biol. Cell Mol. Biol. Cell15, 1991\u20132002.","DOI":"10.1091\/mbc.e03-10-0743"},{"key":"REF25","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-09-0620"},{"key":"REF26","doi-asserted-by":"crossref","unstructured":"Hutchison, C. J. (2002). Lamins: building blocks or regulators of gene expression?Nat. Rev. Mol. Cell. Biol.3, 848\u2013858.","DOI":"10.1038\/nrm950"},{"key":"REF27","doi-asserted-by":"crossref","unstructured":"Iovine, M. K., Watkins, J. L., and Wente, S. R. (1995). The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor.J. Cell Biol.131, 1699\u20131713.","DOI":"10.1083\/jcb.131.6.1699"},{"key":"REF28","doi-asserted-by":"crossref","unstructured":"Kau, T. R., Way, J. C., and Silver, P. A. (2004). Nuclear transport and cancer: from mechanism to intervention.Nat. Rev. Cancer4, 106\u2013117.","DOI":"10.1038\/nrc1274"},{"key":"REF29","doi-asserted-by":"crossref","unstructured":"Kiseleva, E., Allen, T. D., Rutherford, S., Bucci, M., Wente, S. R., and Goldberg, M. W. (2004). Yeast nuclear pore complexes have a cytoplasmic ring and internal filaments.J. Struct. Biol.145, 272\u2013288.","DOI":"10.1016\/j.jsb.2003.11.010"},{"key":"REF30","doi-asserted-by":"crossref","unstructured":"Le Rouzic, E., Mousnier, A., Rustum, C., Stutz, F., Hallberg, E., Dargemont, C., and Benichou, S. (2002). Docking of HIV-1 Vpr to the nuclear envelope is mediated by the interaction with the nucleoporin hCG1.J. Biol. 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Virol.69, 6873\u20136879.","DOI":"10.1128\/JVI.69.11.6873-6879.1995"},{"key":"REF34","doi-asserted-by":"crossref","unstructured":"Lu, Y. L., Spearman, P., and Ratner, L. (1993). Human immunodeficiency virus type 1 viral protein R localization in infected cells and virions.J. Virol.67, 6542\u20136550.","DOI":"10.1128\/JVI.67.11.6542-6550.1993"},{"key":"REF35","doi-asserted-by":"crossref","unstructured":"Mahalingam, S., Ayyavoo, V., Patel, M., Kieber-Emmons, T., and Weiner, D. B. (1997a). Nuclear import, virion incorporation, and cell cycle arrest\/differentiation are mediated by distinct functional domains of human immunodeficiency virus type 1 Vpr.J. Virol.71, 6339\u20136347.","DOI":"10.1128\/JVI.71.9.6339-6347.1997"},{"key":"REF36","doi-asserted-by":"crossref","unstructured":"Mahalingam, S., Collman, R. G., Patel, M., Monken, C. E., and Srinivasan, A. (1995a). 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Sequence analysis of a cDNA encoding a human nuclear pore complex protein, hnup153.Biochim. Biophys. Acta1217, 219\u2013223.","DOI":"10.1016\/0167-4781(94)90040-X"},{"key":"REF40","doi-asserted-by":"crossref","unstructured":"Nakielny, S., Shaikh, S., Burke, B., and Dreyfuss, G. (1999). Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain.EMBO J.18, 1982\u20131995.","DOI":"10.1093\/emboj\/18.7.1982"},{"key":"REF41","doi-asserted-by":"crossref","unstructured":"Pante, N., Bastos, R., McMorrow, I., Burke, B., and Aebi, U. (1994). Interactions and three-dimensional localization of a group of nuclear pore complex proteins.J. Cell Biol.126, 603\u2013617.","DOI":"10.1083\/jcb.126.3.603"},{"key":"REF42","doi-asserted-by":"crossref","unstructured":"Popov, S., Rexach, M., Ratner, L., Blobel, G., and Bukrinsky, M. (1998a). Viral protein R regulates docking of the HIV-1 preintegration complex to the nuclear pore complex.J. Biol. 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Separate nuclear import pathways converge on the nucleoporin Nup153 and can be dissected with dominant-negative inhibitors.Curr. Biol.8, 1376\u20131386.","DOI":"10.1016\/S0960-9822(98)00018-9"},{"key":"REF49","doi-asserted-by":"crossref","unstructured":"Shah, S., Tugendreich, S., and Forbes, D. (1998). Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr.J. Cell Biol.141, 31\u201349.","DOI":"10.1083\/jcb.141.1.31"},{"key":"REF50","doi-asserted-by":"crossref","unstructured":"Sherman, M. P., De Noronha, C. M., Williams, S. A., and Greene, W. C. (2002a). Insights into the biology of HIV-1 viral protein R.DNA Cell Biol.21, 679\u2013688.","DOI":"10.1089\/104454902760330228"},{"key":"REF51","doi-asserted-by":"crossref","unstructured":"Sherman, M. P., Schubert, U., Williams, S. A., de Noronha, C. M., Kreisberg, J. F., Henklein, P., and Greene, W. C. (2002b). 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Role of HIV-1 Vpr in AIDS pathogenesis: relevance and implications of intravirion, intracellular and free Vpr.Biomed. Pharmacother.57, 20\u201324.","DOI":"10.1016\/S0753-3322(02)00328-1"},{"key":"REF58","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.10.3.649"},{"key":"REF59","doi-asserted-by":"crossref","unstructured":"Vasu, S., Shah, S., Orjalo, A., Park, M., Fischer, W. H., and Forbes, D. J. (2001). Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export.J. Cell Biol.155, 339\u2013354.","DOI":"10.1083\/jcb.200108007"},{"key":"REF60","doi-asserted-by":"crossref","unstructured":"Vodicka, M. A., Koepp, D. M., Silver, P. A., and Emerman, M. (1998). HIV-1 Vpr interacts with the nuclear transport pathway to promote macrophage infection.Genes Dev.12, 175\u2013185.","DOI":"10.1101\/gad.12.2.175"},{"key":"REF61","doi-asserted-by":"crossref","unstructured":"Walther, T. C., Fornerod, M., Pickersgill, H., Goldberg, M., Allen, T. D., and Mattaj, I. W. (2001). 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