{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:24:07Z","timestamp":1791177847656,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Cell Biology (ASCB)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p>We previously reported that lipid rafts are involved in long-chain fatty acid (LCFA) uptake in 3T3-L1 adipocytes. The present data show that LCFA uptake does not depend on caveolae endocytosis because expression of a dominant negative mutant of dynamin had no effect on uptake of [<jats:sup>3<\/jats:sup>H]oleic acid, whereas it effectively prevented endocytosis of cholera toxin. Isolation of detergent-resistant membranes (DRMs) from 3T3-L1 cell homogenates revealed that FAT\/CD36 was expressed in both DRMs and detergent-soluble membranes (DSMs), whereas FATP1 and FATP4 were present only in DSMs but not DRMs. Disruption of lipid rafts by cyclodextrin and specific inhibition of FAT\/CD36 by sulfo-N-succinimidyl oleate (SSO) significantly decreased uptake of [<jats:sup>3<\/jats:sup>H]oleic acid, but simultaneous treatment had no additional or synergistic effects, suggesting that both treatments target the same mechanism. Indeed, subcellular fractionation demonstrated that plasma membrane fatty acid translocase (FAT\/CD36) is exclusively located in lipid rafts, whereas intracellular FAT\/CD36 cofractionated with DSMs. Binding assays confirmed that [<jats:sup>3<\/jats:sup>H]SSO predominantly binds to FAT\/CD36 within plasma membrane DRMs. In conclusion, our data strongly suggest that FAT\/CD36 mediates raft-dependent LCFA uptake. Plasma membrane lipid rafts might control LCFA uptake by regulating surface availability of FAT\/CD36.<\/jats:p>","DOI":"10.1091\/mbc.e04-07-0616","type":"journal-article","created":{"date-parts":[[2004,10,21]],"date-time":"2004-10-21T00:13:45Z","timestamp":1098317625000},"page":"24-31","source":"Crossref","is-referenced-by-count":181,"title":["FAT\/CD36-mediated Long-Chain Fatty Acid Uptake in Adipocytes Requires Plasma Membrane Rafts"],"prefix":"10.1091","volume":"16","author":[{"given":"J\u00fcrgen","family":"Pohl","sequence":"first","affiliation":[{"name":"Department of Internal Medicine IV, Ruprechts-Karls-University, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Ring","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV, Ruprechts-Karls-University, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00dcmine","family":"Korkmaz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV, Ruprechts-Karls-University, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Ehehalt","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV, Ruprechts-Karls-University, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Stremmel","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV, Ruprechts-Karls-University, D-69117 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Abumrad, N. 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