{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:13:48Z","timestamp":1761862428124},"reference-count":72,"publisher":"American Society for Cell Biology (ASCB)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p>The actin cytoskeleton controls the overall structure of cells and is highly polarized in chemotaxing cells, with F-actin assembled predominantly in the anterior leading edge and to a lesser degree in the cell's posterior. Wiskott-Aldrich syndrome protein (WASP) has emerged as a central player in controlling actin polymerization. We have investigated WASP function and its regulation in chemotaxing Dictyostelium cells and demonstrated the specific and essential role of WASP in organizing polarized F-actin assembly in chemotaxing cells. Cells expressing very low levels of WASP show reduced F-actin levels and significant defects in polarized F-actin assembly, resulting in an inability to establish axial polarity during chemotaxis. GFP-WASP preferentially localizes at the leading edge and uropod of chemotaxing cells and the B domain of WASP is required for the localization of WASP. We demonstrated that the B domain binds to PI(4,5)P<jats:sub>2<\/jats:sub>and PI(3,4,5)P<jats:sub>3<\/jats:sub>with similar affinities. The interaction between the B domain and PI(3,4,5)P<jats:sub>3<\/jats:sub>plays an important role for the localization of WASP to the leading edge in chemotaxing cells. Our results suggest that the spatial and temporal control of WASP localization and activation is essential for the regulation of directional motility.<\/jats:p>","DOI":"10.1091\/mbc.e04-09-0844","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T01:29:24Z","timestamp":1109208564000},"page":"2191-2206","source":"Crossref","is-referenced-by-count":71,"title":["A<i>Dictyostelium<\/i>Homologue of WASP Is Required for Polarized F-Actin Assembly during Chemotaxis"],"prefix":"10.1091","volume":"16","author":[{"given":"Scott A.","family":"Myers","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Vanderbilt University Medical Center, Nashville TN 37232-6600"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji W.","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Vanderbilt University Medical Center, Nashville TN 37232-6600"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoonsung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Vanderbilt University Medical Center, Nashville TN 37232-6600"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Firtel","sequence":"additional","affiliation":[{"name":"Section of Cell and Developmental Biology, Division of Biological Sciences, Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0634"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang Y.","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Vanderbilt University Medical Center, Nashville TN 37232-6600"},{"name":"Section of Cell and Developmental Biology, Division of Biological Sciences, Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0634"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1076","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Aldrich, R. A., Steinberg, A. G., and Campbell, D. C. (1954). Pedigree demonstrating a sex linked recessive condition characterized by draining ears, eczematoid dermatitis, and bloody diarrhea.Pediatrics13, 133\u2013139.","DOI":"10.1542\/peds.13.2.133"},{"key":"REF2","doi-asserted-by":"crossref","unstructured":"Altman, L. C., Snyderman, R., and Blaese, R. M. (1974). Abnormalities of chemotactic lymphokine synthesis and mononuclear leukocyte chemotaxis in Wiskott-Aldrich syndrome.J. Clin. Invest.54, 486\u2013493.","DOI":"10.1172\/JCI107784"},{"key":"REF3","doi-asserted-by":"crossref","unstructured":"Asano, S., Mishima, M., and Nishida, E. (2001). Coronin forms a stable dimer through its C-terminal coiled coil region: an implicated role in its localization to cell periphery.Genes Cells6, 225\u2013235.","DOI":"10.1046\/j.1365-2443.2001.00416.x"},{"key":"REF4","doi-asserted-by":"crossref","unstructured":"Aubry, L., and Firtel, R. (1999). Integration of signaling networks that regulateDictyosteliumdifferentiation.Annu. Rev. Cell Dev. Biol.15, 469\u20134517.","DOI":"10.1146\/annurev.cellbio.15.1.469"},{"key":"REF5","doi-asserted-by":"crossref","unstructured":"Badolato, R., Sozzani, S., Malacarne, F., Bresciani, S., Fiorini, M., Borsatti, A., Albertini, A., Mantovani, A., Ugazio, A. G., and Notarangelo, L. D. (1998). Monocytes from Wiskott-Aldrich patients display reduced chemotaxis and lack of cell polarization in response to monocyte chemoattractant protein-1 and formyl-methionyl-leucyl-phenylalanine.J. Immunol.161, 1026\u20131033.","DOI":"10.4049\/jimmunol.161.2.1026"},{"key":"REF6","doi-asserted-by":"crossref","unstructured":"Badour, K., Zhang, J., and Siminovitch, K. A. (2003). The Wiskott-Aldrich syndrome protein: forging the link between actin and cell activation.Immunol. Rev.192, 98\u2013112.","DOI":"10.1034\/j.1600-065X.2003.00031.x"},{"key":"REF7","doi-asserted-by":"crossref","unstructured":"Bear, J. E., Rawls, J. F., and Saxe, C. L., 3rd. (1998). SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in lateDictyosteliumdevelopment.J. Cell Biol.142, 1325\u20131335.","DOI":"10.1083\/jcb.142.5.1325"},{"key":"REF8","doi-asserted-by":"crossref","unstructured":"Benesch, S., Lommel, S., Steffen, A., Stradal, T.E.B., Scaplehorn, N., Way, M., Wehland, J., and Rottner, K. (2002). Phosphatidylinositol 4,5-Biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2.J. Biol. Chem.277, 37771\u201337776.","DOI":"10.1074\/jbc.M204145200"},{"key":"REF9","doi-asserted-by":"crossref","unstructured":"Blaauw, M., Linskens, M.H.K., and van Haastert, P.J.M. (2000). Efficient control of gene expression by a tetracycline-dependent transactivator in singleDictyostelium discoideumcells.Gene252, 71\u201382.","DOI":"10.1016\/S0378-1119(00)00227-4"},{"key":"REF10","doi-asserted-by":"crossref","unstructured":"Borisy, G. G., and Svitkina, T. M. (2000). Actin machinery: pushing the envelope.Curr. Opin. Cell Biol.12, 104\u2013112.","DOI":"10.1016\/S0955-0674(99)00063-0"},{"key":"REF11","doi-asserted-by":"crossref","unstructured":"Bresnick, A. R., Warren, V., and Condeelis, J. (1990). Identification of a short sequence essential for actin binding byDictyosteliumABP-120.J. Biol. Chem.265, 9236\u20139240.","DOI":"10.1016\/S0021-9258(19)38837-4"},{"key":"REF12","doi-asserted-by":"crossref","unstructured":"Chung, C. Y., and Firtel, R. A. (1999). PAKa, a putative PAK family member, is required for cytokinesis and the regulation of the cytoskeleton inDictyostelium discoideumcells during chemotaxis.J. Cell Biol.147, 559\u2013575.","DOI":"10.1083\/jcb.147.3.559"},{"key":"REF13","doi-asserted-by":"crossref","unstructured":"Chung, C. Y., Potikyan, G., and Firtel, R. A. (2001). Control of cell polarity and chemotaxis by Akt\/PKB and PI3 kinase through the regulation of PAKa.Mol. Cell.7, 937\u2013947.","DOI":"10.1016\/S1097-2765(01)00247-7"},{"key":"REF14","doi-asserted-by":"crossref","unstructured":"Chung, C. Y., Reddy, T.B.K., Zhou, K. M., and Firtel, R. A. (1998). A novel, putative MEK kinase controls developmental timing and spatial patterning inDictyosteliumand is regulated by ubiquitin-mediated protein degradation.Genes Dev.12, 3564\u20133578.","DOI":"10.1101\/gad.12.22.3564"},{"key":"REF15","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.10.5.1309"},{"key":"REF16","doi-asserted-by":"crossref","unstructured":"Datta, S., and Firtel, R.A. (1987). Identification of the sequences controlling cyclic AMP regulation and cell-type specific expression of a prestalk-specific gene inDictyostelium discoideum.Mol. Cell. Biol.7, 149\u2013159.","DOI":"10.1128\/MCB.7.1.149"},{"key":"REF17","doi-asserted-by":"crossref","unstructured":"de Hostos, E. L. (1999). The coronin family of actin-associated proteins.Trends Cell Biol.9, 345\u2013350.","DOI":"10.1016\/S0962-8924(99)01620-7"},{"key":"REF18","doi-asserted-by":"crossref","unstructured":"Deak, M., Casamayor, A., Currie, R. A., Downes, C. P., and Alessi, D. R. (1999). Characterisation of a plant 3-phosphoinositide-dependent protein kinase-1 homologue which contains a pleckstrin homology domain.FEBS Lett.451, 220\u2013226.","DOI":"10.1016\/S0014-5793(99)00556-6"},{"key":"REF19","unstructured":"Derry, J. M., Ochs, H. D., and Francke, U. (1994). Isolation of a novel gene mutated in Wiskott-Aldrich syndrome.Cell78: 635\u2013644."},{"key":"REF20","doi-asserted-by":"crossref","unstructured":"Devreotes, P., and Janetopoulos, C. (2003). Eukaryotic chemotaxis: distinctions between directional sensing and polarization.J. Biol. Chem.278, 20445\u201320448.","DOI":"10.1074\/jbc.R300010200"},{"key":"REF21","doi-asserted-by":"crossref","unstructured":"Downey, G. P. (1994). Mechanisms of leukocyte motility and chemotaxis.Curr. Opin. Immunol.6, 113\u2013124.","DOI":"10.1016\/0952-7915(94)90042-6"},{"key":"REF22","doi-asserted-by":"crossref","unstructured":"Egelhoff, T. T., Naismith, T. V., and Brozovich, F. V. (1996). Myosin-based cortical tension inDictyosteliumresolved into heavy and light chain-regulated components.J. Muscle Res. Cell Motil.17, 269\u2013274.","DOI":"10.1007\/BF00124248"},{"key":"REF23","doi-asserted-by":"crossref","unstructured":"Firtel, R. A., and Chung, C. Y. (2000). The molecular genetics of chemotaxis: sensing and responding to chemoattractant gradients.BioEssays22, 603\u2013615.","DOI":"10.1002\/1521-1878(200007)22:7<603::AID-BIES3>3.0.CO;2-#"},{"key":"REF24","doi-asserted-by":"crossref","unstructured":"Firtel, R. A., and Meili, R. (2000).Dictyostelium: a model for regulated cell movement during morphogenesis.Curr. Opin. Genet. 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Cell Biol.92, 807\u2013821.","DOI":"10.1083\/jcb.92.3.807"},{"key":"REF28","doi-asserted-by":"crossref","unstructured":"Gerisch, G., Albrecht, R., Heizer, C., Hodgkinson, S., and Maniak, M. (1995). Chemoattractant-controlled accumulation of coronin at the leading edge ofDictyosteliumcells monitored using green fluorescent protein-coronin fusion protein.Curr. Biol.5, 1280\u20131285.","DOI":"10.1016\/S0960-9822(95)00254-5"},{"key":"REF29","doi-asserted-by":"crossref","unstructured":"Hall, A. (1998). Rho GTPases and the actin cytoskeleton.Science279, 509\u2013514.","DOI":"10.1126\/science.279.5350.509"},{"key":"REF30","doi-asserted-by":"crossref","unstructured":"Higgs, H. N., and Pollard, T. D. (2000). Activation by Cdc42 and PIP(2) of Wiskott-Aldrich syndrome protein (WASp) stimulates actin nucleation by Arp2\/3 complex.J. Cell Biol.150, 1311\u20131320.","DOI":"10.1083\/jcb.150.6.1311"},{"key":"REF31","doi-asserted-by":"crossref","unstructured":"Ho, H.Y.H., Rohatgi, R., Lebensohn, A., Ma, L., Li, J., Gygi, S. P., and Kirschner, M. (2004). Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex.Cell118, 203\u2013216.","DOI":"10.1016\/j.cell.2004.06.027"},{"key":"REF32","unstructured":"Hosaka, S., Suzuki, M., and Sato, H. (1979). Leucocyte-like motility of cancer cells, with reference to the mechanism of extravasation in metastasis.Gann70, 559\u2013561."},{"key":"REF33","doi-asserted-by":"crossref","unstructured":"Imai, K., Nonoyama, S., Miki, H., Morio, T., Fukami, K., Zhu, Q., Aruffo, A., Ochs, H.D., Yata, J., and Takenawa, T. (1999). The pleckstrin homology domain of the Wiskott-Aldrich syndrome protein is involved in the organization of actin cytoskeleton.Clin. Immunol.92, 128\u2013137.","DOI":"10.1006\/clim.1999.4746"},{"key":"REF34","unstructured":"Insall, R. H., and Weiner, O. D. (2001). PIP3, PIP2, and cell movement\u2014similar messages, different meanings?Dev. Cell1, 743\u2013747."},{"key":"REF35","doi-asserted-by":"crossref","unstructured":"Kim, A. S., Kakalis, L. T., Abdul-Manan, N., Liu, G. A., and Rosen, M. K. (2000). Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein.Nature404, 151\u2013158.","DOI":"10.1038\/35004513"},{"key":"REF36","doi-asserted-by":"crossref","unstructured":"Lahrtz, F., Piali, L., Spanaus, K. S., Seebach, J., and Fontana, A. (1998). Chemokines and chemotaxis of leukocytes in infectious meningitis.J. Neuroimmunol.85, 33\u201343.","DOI":"10.1016\/S0165-5728(97)00267-1"},{"key":"REF37","doi-asserted-by":"crossref","unstructured":"Li, R. (1997). Bee1, a yeast protein with homology to Wiscott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeleton.J. Cell Biol.136, 649\u2013658.","DOI":"10.1083\/jcb.136.3.649"},{"key":"REF38","unstructured":"Locker, J., Goldblatt, P. J., and Leighton, J. (1970). Ultrastructural features of invasion in chick embryo liver metastasis ofYoshidaascites hepatoma.Cancer Res.30, 1632\u20131644."},{"key":"REF39","doi-asserted-by":"crossref","unstructured":"Machesky, L. M., and Insall, R. H. (1998). Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2\/3 complex.Curr. Biol.8, 1347\u20131356.","DOI":"10.1016\/S0960-9822(98)00015-3"},{"key":"REF40","doi-asserted-by":"crossref","unstructured":"Machesky, L. M., Mullins, R. D., Higgs, H. N., Kaiser, D. A., Blanchoin, L., May, R. C., Hall, M. E., and Pollard, T. D. (1999). Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2\/3 complex.Proc. Natl. Acad. Sci. USA96, 3739\u20133744.","DOI":"10.1073\/pnas.96.7.3739"},{"key":"REF41","doi-asserted-by":"crossref","unstructured":"Marchand, J. B., Kaiser, D. A., Pollard, T. D., and Higgs, H. N. (2001). Interaction of WASP\/Scar proteins with actin and vertebrate Arp2\/3 complex.Nat. Cell Biol.3, 76\u201382.","DOI":"10.1038\/35050590"},{"key":"REF42","doi-asserted-by":"crossref","unstructured":"Meili, R., Ellsworth, C., Lee, S., Reddy, T.B.K., Ma, H., and Firtel, R. A. (1999). Chemoattractant-mediated transient activation and membrane localization of Akt\/PKB is required for efficient chemotaxis to cAMP inDictyostelium.EMBO J.18, 2092\u20132105.","DOI":"10.1093\/emboj\/18.8.2092"},{"key":"REF43","doi-asserted-by":"crossref","unstructured":"Merlot, S., and Firtel, R. A. (2003). Leading the way: directional sensing through phosphatidylinositol 3-kinase and other signaling pathways.J. Cell Sci.116, 3471\u20133478.","DOI":"10.1242\/jcs.00703"},{"key":"REF44","doi-asserted-by":"crossref","unstructured":"Miki, H., Miura, K., and Takenawa, T. (1996). N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases.EMBO J.15, 5326\u20135335.","DOI":"10.1002\/j.1460-2075.1996.tb00917.x"},{"key":"REF45","unstructured":"Ochs, H. D. (1998a). The Wiskott-Aldrich syndrome.Semin. Hematol.35, 332\u2013345."},{"key":"REF46","doi-asserted-by":"crossref","unstructured":"Ochs, H. D. (1998b). The Wiskott-Aldrich syndrome. Springer Semin.Immunopathol.19, 435\u2013458.","DOI":"10.1007\/BF00792601"},{"key":"REF47","doi-asserted-by":"crossref","unstructured":"Oikawa, T., Yamaguchi, H., Itoh, T., Kato, M., Ijuin, T., Yamazaki, D., Suetsugu, S., and Takenawa, T. (2004). PtdIns(3,4,5)P3binding is necessary for WAVE2-induced formation of lamellipodia.Nat. Cell Biol.6, 420\u2013428.","DOI":"10.1038\/ncb1125"},{"key":"REF48","doi-asserted-by":"crossref","unstructured":"Pang, K. M., Lee, E., and Knecht, D. A. (1998). Use of a fusion protein between GFP and an actin-binding domain to visualize transient filamentous-actin structures.Curr. Biol.8, 405\u2013408.","DOI":"10.1016\/S0960-9822(98)70159-9"},{"key":"REF49","doi-asserted-by":"crossref","unstructured":"Parent, C. A., Blacklock, B. J., Froehlich, W. M., Murphy, D. B., and Devreotes, P. N. (1998). G protein signaling events are activated at the leading edge of chemotactic cells.Cell95, 81\u201391.","DOI":"10.1016\/S0092-8674(00)81784-5"},{"key":"REF50","doi-asserted-by":"crossref","unstructured":"Parent, C. A., and Devreotes, P. N. (1999). A cell's sense of direction.Science284, 765\u2013770.","DOI":"10.1126\/science.284.5415.765"},{"key":"REF51","doi-asserted-by":"crossref","unstructured":"Prehoda, K. E., Scott, J. A., Mullins, R. D., and Lim, W. A. (2000). Integration of multiple signals through cooperative regulation of the N-WASP-Arp2\/3 complex.Science290, 801\u2013806.","DOI":"10.1126\/science.290.5492.801"},{"key":"REF52","doi-asserted-by":"crossref","unstructured":"Remold-O'Donnell, E., Cooley, J., Shcherbina, A., Hagemann, T. L., Kwan, S. P., Kenney, D. M., and Rosen, F. S. (1997). Variable expression of WASP in B cell lines of Wiskott-Aldrich syndrome patients.J. Immunol.158, 4021\u20134025.","DOI":"10.4049\/jimmunol.158.9.4021"},{"key":"REF53","doi-asserted-by":"crossref","unstructured":"Rickert, P., Weiner, O. D., Wang, F., Bourne, H. R., and Servant, G. (2000). Leukocytes navigate by compass: roles of PI3K[gamma] and its lipid products.Trends Cell Biol.10, 466\u2013473.","DOI":"10.1016\/S0962-8924(00)01841-9"},{"key":"REF54","doi-asserted-by":"crossref","unstructured":"Rodal, A. A., Manning, A. L., Goode, B. L., and Drubin, D. G. (2003). Negative regulation of yeast WASp by two SH3 domain-containing proteins.Curr. 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D., Herzmark, P., Balla, T., Sedat, J. W., and Bourne, H. R. (2000). Polarization of chemoattractant receptor signaling during neutrophil chemotaxis.Science287, 1037\u20131040.","DOI":"10.1126\/science.287.5455.1037"},{"key":"REF59","doi-asserted-by":"crossref","unstructured":"Snapper, S. B.et al.(1998). Wiskott-Aldrich syndrome protein-deficient mice reveal a role for WASP in T but not B cell activation.Immunity9, 81\u201391.","DOI":"10.1016\/S1074-7613(00)80590-7"},{"key":"REF60","doi-asserted-by":"crossref","unstructured":"Stites, J., Wessels, D., Uhl, A., Egelhoff, T., Shutt, D., and Soll, D. R. (1998). Phosphorylation of theDictyosteliummyosin II heavy chain is necessary for maintaining cellular polarity and suppressing turning during chemotaxis.Cell Motil. Cytoskeleton39, 31\u201351.","DOI":"10.1002\/(SICI)1097-0169(1998)39:1<31::AID-CM4>3.0.CO;2-J"},{"key":"REF61","doi-asserted-by":"crossref","unstructured":"Symons, M., Derry, J. M., Karlak, B., Jiang, S., Lemahieu, V., McCormick, F., Francke, U., and Abo, A. (1996). Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization.Cell84, 723\u2013734.","DOI":"10.1016\/S0092-8674(00)81050-8"},{"key":"REF62","doi-asserted-by":"crossref","unstructured":"Thrasher, A. J., Burns, S., Lorenzi, R., and Jones, G. E. (2000). The Wiskott-Aldrich syndrome: disordered actin dynamics in haematopoietic cells.Immunol. Rev.178, 118\u2013128.","DOI":"10.1034\/j.1600-065X.2000.17803.x"},{"key":"REF63","doi-asserted-by":"crossref","unstructured":"Wang, F., Herzmark, P., Weiner, O. D., Srinivasan, S., Servant, G., and Bourne, H. R. (2002). Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophils.Nat. Cell Biol.4, 513\u2013518.","DOI":"10.1038\/ncb810"},{"key":"REF64","doi-asserted-by":"crossref","unstructured":"Weijer, C.J. (1999). Morphogenetic cell movement inDictyostelium.Semin. Cell Dev. 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