{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:54:17Z","timestamp":1789710857656,"version":"4.0.1"},"reference-count":41,"publisher":"American Society for Cell Biology (ASCB)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p> In fission yeast, calcineurin dephosphorylates and activates the Prz1 transcription factor. Here, we identified the calcineurin-dependent response element (CDRE) in the promoter region of prz1<jats:sup>+<\/jats:sup> gene and monitored the calcineurin activity in living cells using a destabilized luciferase reporter gene fused to three tandem repeats of CDRE. Elevated extracellular CaCl<jats:sub>2<\/jats:sub> caused an increase in calcineurin activity with an initial peak and then approached a sustained constant level in a concentration-dependent manner. In CaCl<jats:sub>2<\/jats:sub>-sensitive mutants such as \u0394pmc1, the response was markedly enhanced, reflecting its high intracellular Ca<jats:sup>2+<\/jats:sup>. Agents expected to induce Ca<jats:sup>2+<\/jats:sup> influx showed distinct patterns of the CDRE-reporter activity, suggesting different mechanisms of calcineurin activation. Knockout of yam8<jats:sup>+<\/jats:sup> or cch1<jats:sup>+<\/jats:sup> encoding putative subunits of a Ca<jats:sup>2+<\/jats:sup> channel abolished the activation of calcineurin upon exposure to various stimuli, including high extracellular NaCl and cell wall\u2013damaging agents. However, knockout of yam8<jats:sup>+<\/jats:sup> or cch1<jats:sup>+<\/jats:sup> did not affect the activation of calcineurin upon stimulation by elevated extracellular Ca<jats:sup>2+<\/jats:sup>. The Pck2 protein kinase C-Pmk1 mitogen-activate protein kinase pathway was required for the stimulation of calcineurin via Yam8\/Cch1-mediated Ca<jats:sup>2+<\/jats:sup> influx, but it was not required for the stimulation by elevated extracellular Ca<jats:sup>2+<\/jats:sup>, suggesting two distinct pathways for calcineurin activation. <\/jats:p>","DOI":"10.1091\/mbc.e06-06-0526","type":"journal-article","created":{"date-parts":[[2006,8,23]],"date-time":"2006-08-23T20:59:24Z","timestamp":1156366764000},"page":"4790-4800","source":"Crossref","is-referenced-by-count":58,"title":["Real-Time Monitoring of Calcineurin Activity in Living Cells: Evidence for Two Distinct Ca<sup>2+<\/sup>-dependent Pathways in Fission Yeast"],"prefix":"10.1091","volume":"17","author":[{"given":"Lu","family":"Deng","sequence":"first","affiliation":[{"name":"*Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reiko","family":"Sugiura","sequence":"additional","affiliation":[{"name":"*Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan; and"},{"name":"Laboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kinki University, Higashi-Osaka 577-8502, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"*Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Suzuki","sequence":"additional","affiliation":[{"name":"*Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidemine","family":"Ebina","sequence":"additional","affiliation":[{"name":"*Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomonori","family":"Takami","sequence":"additional","affiliation":[{"name":"*Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Koike","sequence":"additional","affiliation":[{"name":"*Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiori","family":"Iba","sequence":"additional","affiliation":[{"name":"*Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayoshi","family":"Kuno","sequence":"additional","affiliation":[{"name":"*Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0378-1119(93)90552-E","volume":"123","author":"Basi G.","year":"1993","journal-title":"Gene"},{"key":"B2","doi-asserted-by":"crossref","first-page":"23357","DOI":"10.1074\/jbc.271.38.23357","volume":"271","author":"Batiza A. 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