{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T21:11:31Z","timestamp":1790975491489,"version":"4.1.0"},"reference-count":83,"publisher":"American Society for Cell Biology (ASCB)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p>The intracellular trafficking of the epidermal growth factor receptor (EGFR) is regulated by a cross-talk between calmodulin (CaM) and protein kinase C\u03b4 (PKC\u03b4). On inhibition of CaM, PKC\u03b4 promotes the formation of enlarged early endosomes and blocks EGFR recycling and degradation. Here, we show that PKC\u03b4 impairs EGFR trafficking due to the formation of an F-actin coat surrounding early endosomes. The PKC\u03b4-induced polymerization of actin is orchestrated by the Arp2\/3 complex and requires the interaction of cortactin with PKC\u03b4. Accordingly, inhibition of actin polymerization by using cytochalasin D or by overexpression of active cofilin, restored the normal morphology of the organelle and the recycling of EGFR. Similar results were obtained after down-regulation of cortactin and the sequestration of the Arp2\/3 complex. Furthermore we demonstrate an interaction of cortactin with CaM and PKC\u03b4, the latter being dependent on CaM inhibition. In summary, this study provides the first evidence that CaM and PKC\u03b4 organize actin dynamics in the early endosomal compartment, thereby regulating the intracellular trafficking of EGFR.<\/jats:p>","DOI":"10.1091\/mbc.e07-05-0411","type":"journal-article","created":{"date-parts":[[2007,10,24]],"date-time":"2007-10-24T23:20:33Z","timestamp":1193268033000},"page":"17-29","source":"Crossref","is-referenced-by-count":41,"title":["Protein Kinase C\u03b4 and Calmodulin Regulate Epidermal Growth Factor Receptor Recycling from Early Endosomes through Arp2\/3 Complex and Cortactin"],"prefix":"10.1091","volume":"19","author":[{"given":"Anna","family":"Llad\u00f3","sequence":"first","affiliation":[{"name":"*Departament de Biologia Cel\u00b7lular, Facultat de Medicina, Universitat de Barcelona, Casanova 143, 08036-Barcelona, Spain;"},{"name":"Unitat de Microscopia Confocal, Facultat de Medicina, Universitat de Barcelona, 08036 Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Timpson","sequence":"additional","affiliation":[{"name":"The Garvan Institute of Medical Research, Darlinghurst, Sydney, NSW 2010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Vil\u00e0 de Muga","sequence":"additional","affiliation":[{"name":"*Departament de Biologia Cel\u00b7lular, Facultat de Medicina, Universitat de Barcelona, Casanova 143, 08036-Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jemina","family":"Moret\u00f3","sequence":"additional","affiliation":[{"name":"*Departament de Biologia Cel\u00b7lular, Facultat de Medicina, Universitat de Barcelona, Casanova 143, 08036-Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Pol","sequence":"additional","affiliation":[{"name":"Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS), Facultat de Medicina, Universitat de Barcelona, 08036-Barcelona, Spain;"},{"name":"Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats (ICREA), 08010-Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Grewal","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Sydney, Sydney, NSW 2010, Australia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger J.","family":"Daly","sequence":"additional","affiliation":[{"name":"The Garvan Institute of Medical Research, Darlinghurst, Sydney, NSW 2010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Enrich","sequence":"additional","affiliation":[{"name":"*Departament de Biologia Cel\u00b7lular, Facultat de Medicina, Universitat de Barcelona, Casanova 143, 08036-Barcelona, Spain;"},{"name":"Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS), Facultat de Medicina, Universitat de Barcelona, 08036-Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesc","family":"Tebar","sequence":"additional","affiliation":[{"name":"*Departament de Biologia Cel\u00b7lular, Facultat de Medicina, Universitat de Barcelona, Casanova 143, 08036-Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1083\/jcb.119.3.617","volume":"119","author":"Adamson P.","year":"1992","journal-title":"J. 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