{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:10:37Z","timestamp":1791169837784,"version":"4.1.0"},"reference-count":66,"publisher":"American Society for Cell Biology (ASCB)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:p>The assembly of metazoan Sm-class small nuclear ribonucleoproteins (snRNPs) is an elaborate, step-wise process that takes place in multiple subcellular compartments. The initial steps, including formation of the core RNP, are mediated by the survival motor neuron (SMN) protein complex. Loss-of-function mutations in human SMN1 result in a neuromuscular disease called spinal muscular atrophy. The SMN complex is comprised of SMN and a number of tightly associated proteins, collectively called Gemins. In this report, we identify and characterize the fruitfly ortholog of the DEAD box protein, Gemin3. Drosophila Gemin3 (dGem3) colocalizes and interacts with dSMN in vitro and in vivo. RNA interference for dGem3 codepletes dSMN and inhibits efficient Sm core assembly in vitro. Transposon insertion mutations in Gemin3 are larval lethals and also codeplete dSMN. Transgenic overexpression of dGem3 rescues lethality, but overexpression of dSMN does not, indicating that loss of dSMN is not the primary cause of death. Gemin3 mutant larvae exhibit motor defects similar to previously characterized Smn alleles. Remarkably, appreciable numbers of Gemin3 mutants (along with one previously undescribed Smn allele) survive as larvae for several weeks without pupating. Our results demonstrate the conservation of Gemin3 protein function in metazoan snRNP assembly and reveal that loss of either Smn or Gemin3 can contribute to neuromuscular dysfunction.<\/jats:p>","DOI":"10.1091\/mbc.e08-01-0024","type":"journal-article","created":{"date-parts":[[2008,10,16]],"date-time":"2008-10-16T03:54:21Z","timestamp":1224129261000},"page":"90-101","source":"Crossref","is-referenced-by-count":58,"title":["<i>Gemin3<\/i>Is an Essential Gene Required for Larval Motor Function and Pupation in<i>Drosophila<\/i>"],"prefix":"10.1091","volume":"20","author":[{"given":"Karl B.","family":"Shpargel","sequence":"first","affiliation":[{"name":"*Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106-4955; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavita","family":"Praveen","sequence":"additional","affiliation":[{"name":"Departments of Biology and Genetics, Program in Molecular Biology and Biotechnology, Curriculum in Genetics and Molecular Biology, and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599-3280"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. 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