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We identify PAT-3::GFP, the \u03b2 subunit of the heterodimeric integrin receptors, as an RNF-121 substrate; whereas induction of RNF-121 expression reduced the level of PAT-3::GFP in the gonad distal tip cells, inhibition of RNF-121 led to the accumulation of stably bound PAT-3::GFP inclusions. Correspondingly, overexpression of RNF-121 during early stages of gonad development led to aberrations in germline development and gonad migration that overlap with those observed after PAT-3 inactivation. The formation of these gonad abnormalities required functional ER-associated degradation (ERAD) machinery. Our findings identify RNF-121 as an ER-anchored ubiquitin ligase that plays a specific role in the ERAD pathway by linking it to the regulation of the cell adhesion integrin receptors.<\/jats:p>","DOI":"10.1091\/mbc.e09-09-0774","type":"journal-article","created":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T01:54:15Z","timestamp":1270086855000},"page":"1788-1798","source":"Crossref","is-referenced-by-count":40,"title":["RNF-121 Is an Endoplasmic Reticulum-Membrane E3 Ubiquitin Ligase Involved in the Regulation of \u03b2-Integrin"],"prefix":"10.1091","volume":"21","author":[{"given":"Amir","family":"Darom","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Bening-Abu-Shach","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limor","family":"Broday","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"2436","DOI":"10.1101\/gad.1096603","volume":"17","author":"Amano T.","year":"2003","journal-title":"Genes Dev"},{"key":"B2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/genetics\/77.1.71","volume":"77","author":"Brenner S.","year":"1974","journal-title":"Genetics"},{"key":"B3","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.1002\/dvdy.21341","volume":"236","author":"Broday L.","year":"2007","journal-title":"Dev. 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