{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:58:39Z","timestamp":1791302319478,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Cell Biology (ASCB)","issue":"19","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:p> Hereditary spastic paraplegias (HSPs, SPG1-46) are inherited neurological disorders characterized by lower extremity spastic weakness. Loss-of-function SPG20 gene mutations cause an autosomal recessive HSP known as Troyer syndrome. The SPG20 protein spartin localizes to lipid droplets and endosomes, and it interacts with tail interacting protein 47 (TIP47) as well as the ubiquitin E3 ligases atrophin-1-interacting protein (AIP)4 and AIP5. Spartin harbors a domain contained within microtubule-interacting and trafficking molecules (MIT) at its N-terminus, and most proteins with MIT domains interact with specific ESCRT-III proteins. Using yeast two-hybrid and in vitro surface plasmon resonance assays, we demonstrate that the spartin MIT domain binds with micromolar affinity to the endosomal sorting complex required for transport (ESCRT)-III protein increased sodium tolerance (Ist)1 but not to ESCRT-III proteins charged multivesicular body proteins 1\u20137. Spartin colocalizes with Ist1 at the midbody, and depletion of Ist1 in cells by small interfering RNA significantly decreases the number of cells where spartin is present at midbodies. Depletion of spartin does not affect Ist1 localization to midbodies but markedly impairs cytokinesis. A structure-based amino acid substitution in the spartin MIT domain (F24D) blocks the spartin\u2013Ist1 interaction. Spartin F24D does not localize to the midbody and acts in a dominant-negative manner to impair cytokinesis. These data suggest that Ist1 interaction is important for spartin recruitment to the midbody and that spartin participates in cytokinesis. <\/jats:p>","DOI":"10.1091\/mbc.e09-10-0879","type":"journal-article","created":{"date-parts":[[2010,8,19]],"date-time":"2010-08-19T01:57:54Z","timestamp":1282183074000},"page":"3293-3303","source":"Crossref","is-referenced-by-count":80,"title":["SPG20 Protein Spartin Is Recruited to Midbodies by ESCRT-III Protein Ist1 and Participates in Cytokinesis"],"prefix":"10.1091","volume":"21","author":[{"given":"Beno\u00eet","family":"Renvois\u00e9","sequence":"first","affiliation":[{"name":"*Cellular Neurology Unit, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rell L.","family":"Parker","sequence":"additional","affiliation":[{"name":"*Cellular Neurology Unit, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke and"},{"name":"Howard Hughes Medical Institute-National Institutes of Health Research Scholars Program, Bethesda, MD 20814; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna C.","family":"Bakowska","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, Loyola University Chicago Stritch School of Medicine, Maywood, IL 60153"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James H.","family":"Hurley","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Blackstone","sequence":"additional","affiliation":[{"name":"*Cellular Neurology Unit, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e08-05-0474"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e08-05-0475"},{"key":"B3","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1038\/nsmb.1621","volume":"16","author":"Bajorek M.","year":"2009","journal-title":"Nat. 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