{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:47:03Z","timestamp":1792716423521,"version":"4.5.0"},"reference-count":62,"publisher":"American Society for Cell Biology (ASCB)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2010,3,15]]},"abstract":"<jats:p>The significance and extent of Ca<jats:sup>2+<\/jats:sup>regulation of the biosynthetic secretory pathway have been difficult to establish, and our knowledge of regulatory relationships integrating Ca<jats:sup>2+<\/jats:sup>with vesicle coats and function is rudimentary. Here, we investigated potential roles and mechanisms of luminal Ca<jats:sup>2+<\/jats:sup>in the early secretory pathway. Specific depletion of luminal Ca<jats:sup>2+<\/jats:sup>in living normal rat kidney cells using cyclopiazonic acid (CPA) resulted in the extreme expansion of vesicular tubular cluster (VTC) elements. Consistent with this, a suppressive role for vesicle-associated Ca<jats:sup>2+<\/jats:sup>in COPII vesicle homotypic fusion was demonstrated in vitro using Ca<jats:sup>2+<\/jats:sup>chelators. The EF-hand\u2013containing protein apoptosis-linked gene 2 (ALG-2), previously implicated in the stabilization of sec31 at endoplasmic reticulum exit sites, inhibited COPII vesicle fusion in a Ca<jats:sup>2+<\/jats:sup>-requiring manner, suggesting that ALG-2 may be a sensor for the effects of vesicular Ca<jats:sup>2+<\/jats:sup>on homotypic fusion. Immunoisolation established that Ca<jats:sup>2+<\/jats:sup>chelation inhibits and ALG-2 specifically favors residual retention of the COPII outer shell protein sec31 on pre-Golgi fusion intermediates. We conclude that vesicle-associated Ca<jats:sup>2+<\/jats:sup>, acting through ALG-2, favors the retention of residual coat molecules that seem to suppress membrane fusion. We propose that in cells, these Ca<jats:sup>2+<\/jats:sup>-dependent mechanisms temporally regulate COPII vesicle interactions, VTC biogenesis, cargo sorting, and VTC maturation.<\/jats:p>","DOI":"10.1091\/mbc.e09-10-0914","type":"journal-article","created":{"date-parts":[[2010,1,21]],"date-time":"2010-01-21T03:25:30Z","timestamp":1264044330000},"page":"1033-1046","source":"Crossref","is-referenced-by-count":50,"title":["Vesicular Calcium Regulates Coat Retention, Fusogenicity, and Size of Pre-Golgi Intermediates"],"prefix":"10.1091","volume":"21","author":[{"given":"Marvin","family":"Bentley","sequence":"first","affiliation":[{"name":"*Division of Biological Sciences and Center for Structural and Functional Neuroscience, The University of Montana, Missoula, MT 59812-4824;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah C.","family":"Nycz","sequence":"additional","affiliation":[{"name":"*Division of Biological Sciences and Center for Structural and Functional Neuroscience, The University of Montana, Missoula, MT 59812-4824;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashwini","family":"Joglekar","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismene","family":"Fertschai","sequence":"additional","affiliation":[{"name":"Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Malli","sequence":"additional","affiliation":[{"name":"Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang F.","family":"Graier","sequence":"additional","affiliation":[{"name":"Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse C.","family":"Hay","sequence":"additional","affiliation":[{"name":"*Division of Biological Sciences and Center for Structural and Functional Neuroscience, The University of Montana, Missoula, MT 59812-4824;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"34148","DOI":"10.1074\/jbc.M105398200","volume":"276","author":"Ahluwalia J. 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