{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T12:36:29Z","timestamp":1793104589923,"version":"4.5.0"},"reference-count":46,"publisher":"American Society for Cell Biology (ASCB)","issue":"15","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:p> Lafora progressive myoclonus epilepsy is a fatal neurodegenerative disorder caused by defects in the function of at least two proteins: laforin, a dual-specificity protein phosphatase, and malin, an E3-ubiquitin ligase. In this study, we report that a functional laforin\u2013malin complex promotes the ubiquitination of AMP-activated protein kinase (AMPK), a serine\/threonine protein kinase that acts as a sensor of cellular energy status. This reaction occurs when any of the three AMPK subunits (\u03b1, \u03b2, and \u03b3) are expressed individually in the cell, and it also occurs on AMPK\u03b2 when it is part of a heterotrimeric complex. We also report that the laforin\u2013malin complex promotes the formation of K63-linked ubiquitin chains, which are not involved in proteasome degradation. On the contrary, this modification increases the steady-state levels of at least AMPK\u03b2 subunit, possibly because it leads to the accumulation of this protein into inclusion bodies. These results suggest that the modification introduced by the laforin\u2013malin complex could affect the subcellular distribution of AMPK\u03b2 subunits. <\/jats:p>","DOI":"10.1091\/mbc.e10-03-0227","type":"journal-article","created":{"date-parts":[[2010,6,10]],"date-time":"2010-06-10T02:20:18Z","timestamp":1276136418000},"page":"2578-2588","source":"Crossref","is-referenced-by-count":61,"title":["The Laforin\u2013Malin Complex, Involved in Lafora Disease, Promotes the Incorporation of K63-linked Ubiquitin Chains into AMP-activated Protein Kinase \u03b2 Subunits"],"prefix":"10.1091","volume":"21","author":[{"given":"Daniel","family":"Moreno","sequence":"first","affiliation":[{"name":"*Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas and Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras, 46010 Valencia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mhairi C.","family":"Towler","sequence":"additional","affiliation":[{"name":"Division of Molecular Physiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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