{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T07:10:38Z","timestamp":1781766638866,"version":"3.54.5"},"reference-count":46,"publisher":"American Society for Cell Biology (ASCB)","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p> Cell migration requires a tightly regulated, spatiotemporal coordination of underlying biochemical pathways. Crucial to cell migration is SCAR\/WAVE\u2013mediated dendritic F-actin polymerization at the cell's leading edge. Our goal is to understand the role the SCAR\/WAVE complex plays in the mechanics of amoeboid migration. To this aim, we measured and compared the traction stresses exerted by Dictyostelium cells lacking the SCAR\/WAVE complex proteins PIR121 (pirA<jats:sup>\u2212<\/jats:sup>) and SCAR (scrA<jats:sup>\u2212<\/jats:sup>) with those of wild-type cells while they were migrating on flat, elastic substrates. We found that, compared to wild type, both mutant strains exert traction stresses of different strengths that correlate with their F-actin levels. In agreement with previous studies, we found that wild-type cells migrate by repeating a motility cycle in which the cell length and strain energy exerted by the cells on their substrate vary periodically. Our analysis also revealed that scrA<jats:sup>\u2212<\/jats:sup> cells display an altered motility cycle with a longer period and a lower migration velocity, whereas pirA<jats:sup>\u2212<\/jats:sup> cells migrate in a random manner without implementing a periodic cycle. We present detailed characterization of the traction-stress phenotypes of the various cell lines, providing new insights into the role of F-actin polymerization in regulating cell\u2013substratum interactions and stresses required for motility. <\/jats:p>","DOI":"10.1091\/mbc.e11-03-0278","type":"journal-article","created":{"date-parts":[[2011,9,7]],"date-time":"2011-09-07T22:46:55Z","timestamp":1315435615000},"page":"3995-4003","source":"Crossref","is-referenced-by-count":25,"title":["The SCAR\/WAVE complex is necessary for proper regulation of traction stresses during amoeboid motility"],"prefix":"10.1091","volume":"22","author":[{"given":"Effie","family":"Bastounis","sequence":"first","affiliation":[{"name":"Department of Bioengineering, Jacobs School of Engineering, University of California, San Diego, La Jolla, CA 92093"},{"name":"Department of Mechanical and Aerospace Engineering, Jacobs School of Engineering, University of California, San Diego, La Jolla, CA 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