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Depletion of ALBA3\/4 proteins by RNA interference in the cultured procyclic stage produces cell modifications mimicking several morphogenetic aspects of trypanosome differentiation that usually take place in the fly midgut. A combination of immunofluorescence data and videomicroscopy analysis of live trypanosomes expressing endogenously ALBA fused with fluorescent proteins revealed that ALBA3\/4 are present throughout the development of the parasite in the tsetse fly, with the striking exception of the transition stages found in the proventriculus region. This involves migration of the nucleus toward the posterior end of the cell, a phenomenon that is perturbed upon forced expression of ALBA3 during the differentiation process, showing for the first time the involvement of an RNA-binding protein in trypanosome development in vivo.<\/jats:p>","DOI":"10.1091\/mbc.e11-06-0511","type":"journal-article","created":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T04:22:33Z","timestamp":1317442953000},"page":"4205-4219","source":"Crossref","is-referenced-by-count":99,"title":["ALBA proteins are stage regulated during trypanosome development in the tsetse fly and participate in differentiation"],"prefix":"10.1091","volume":"22","author":[{"given":"Ines","family":"Subota","sequence":"first","affiliation":[{"name":"Trypanosome Cell Biology Unit, Centre National de la Recherche Scientifique (URA 2581), Parasitology and Mycology Department, Institut Pasteur, 75015 Paris, 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