{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:09:14Z","timestamp":1792714154646,"version":"4.5.0"},"reference-count":59,"publisher":"American Society for Cell Biology (ASCB)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2012,1,15]]},"abstract":"<jats:p>Invadosomes are F-actin structures capable of degrading the matrix through the activation of matrix metalloproteases. As fibrillar type I collagen promotes pro-matrix metalloproteinase 2 activation by membrane type 1 matrix metalloproteinase, we aimed at investigating the functional relationships between collagen I organization and invadosome induction. We found that fibrillar collagen I induced linear F-actin structures, distributed along the fibrils, on endothelial cells, macrophages, fibroblasts, and tumor cells. These structures share features with conventional invadosomes, as they express cortactin and N-WASP and accumulate the scaffold protein Tks5, which proved essential for their formation. On the basis of their ability to degrade extracellular matrix elements and their original architecture, we named these structures \u201clinear invadosomes.\u201d Interestingly, podosomes or invadopodia were replaced by linear invadosomes upon contact of the cells with fibrillar collagen I. However, linear invadosomes clearly differ from classical invadosomes, as they do not contain paxillin, vinculin, and \u03b21\/\u03b23 integrins. Using knockout mouse embryonic fibroblasts and RGD peptide, we demonstrate that linear invadosome formation and activity are independent of \u03b21 and \u03b23 integrins. Finally, linear invadosomes also formed in a three-dimensional collagen matrix. This study demonstrates that fibrillar collagen I is the physiological inducer of a novel class of invadosomes.<\/jats:p>","DOI":"10.1091\/mbc.e11-07-0594","type":"journal-article","created":{"date-parts":[[2011,11,24]],"date-time":"2011-11-24T05:55:38Z","timestamp":1322114138000},"page":"297-309","source":"Crossref","is-referenced-by-count":119,"title":["Physiological type I collagen organization induces the formation of a novel class of linear invadosomes"],"prefix":"10.1091","volume":"23","author":[{"given":"Am\u00e9lie","family":"Juin","sequence":"first","affiliation":[{"name":"INSERM, U1053, F-33076 Bordeaux, France"},{"name":"Universit\u00e9 Bordeaux Segalen, F-33076 Bordeaux, France"},{"name":"European Institute of Chemistry and Biology, F-33600 Pessac, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clotilde","family":"Billottet","sequence":"additional","affiliation":[{"name":"INSERM, U1053, F-33076 Bordeaux, France"},{"name":"Universit\u00e9 Bordeaux 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