{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T01:12:09Z","timestamp":1793236329997,"version":"4.5.0"},"reference-count":54,"publisher":"American Society for Cell Biology (ASCB)","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:p>During clathrin-mediated endocytosis, branched actin polymerization nucleated by the Arp2\/3 complex provides force needed to drive vesicle internalization. Las17 (yeast WASp) is the strongest activator of the Arp2\/3 complex in yeast cells; it is not autoinhibited and arrives to endocytic sites 20 s before actin polymerization begins. It is unclear how Las17 is kept inactive for 20 s at endocytic sites, thus restricting actin polymerization to late stages of endocytosis. In this paper, we demonstrate that Las17 is part of a large and biochemically stable complex with Sla1, a clathrin adaptor that inhibits Las17 activity. The interaction is direct, multivalent, and strong, and was mapped to novel Las17 polyproline motifs that are simultaneously class I and class II. In vitro pyrene-actin polymerization assays established that Sla1 inhibition of Las17 activity depends on the class I\/II Las17 polyproline motifs and is based on competition between Sla1 and monomeric actin for binding to Las17. Furthermore, live-cell imaging showed the interaction with Sla1 is important for normal Las17 recruitment to endocytic sites, inhibition during the initial 20 s, and efficient endocytosis. These results advance our understanding of the regulation of actin polymerization in endocytosis.<\/jats:p>","DOI":"10.1091\/mbc.e11-12-1022","type":"journal-article","created":{"date-parts":[[2012,9,13]],"date-time":"2012-09-13T02:31:48Z","timestamp":1347503508000},"page":"4256-4272","source":"Crossref","is-referenced-by-count":65,"title":["SLAC, a complex between Sla1 and Las17, regulates actin polymerization during clathrin-mediated endocytosis"],"prefix":"10.1091","volume":"23","author":[{"given":"Daniel","family":"Feliciano","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago M.","family":"Di Pietro","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.10.4.1061"},{"key":"B2","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1016\/j.cub.2009.10.062","volume":"19","author":"Boettner DR","year":"2009","journal-title":"Curr Biol"},{"key":"B3","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1038\/ncb2307","volume":"13","author":"Boulant S","year":"2011","journal-title":"Nat Cell Biol"},{"key":"B4","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1016\/j.cub.2011.05.048","volume":"21","author":"Collins A","year":"2011","journal-title":"Curr Biol"},{"key":"B5","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1042\/BST0331273","volume":"33","author":"Costa R","year":"2005","journal-title":"Biochem Soc Trans"},{"key":"B6","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1038\/emboj.2010.5","volume":"29","author":"Di Pietro SM","year":"2010","journal-title":"EMBO J"},{"key":"B7","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1111\/j.1600-0854.2004.0171.x","volume":"5","author":"Di Pietro SM","year":"2004","journal-title":"Traffic"},{"key":"B8","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1146\/annurev.biochem.78.081307.110540","volume":"78","author":"Doherty GJ","year":"2009","journal-title":"Annu Rev Biochem"},{"key":"B9","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1038\/35083087","volume":"3","author":"Duncan MC","year":"2001","journal-title":"Nat Cell Biol"},{"key":"B10","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1146\/annurev.cellbio.19.111401.093127","volume":"19","author":"Engqvist-Goldstein AE","year":"2003","journal-title":"Annu Rev Cell Dev Biol"},{"key":"B11","doi-asserted-by":"crossref","first-page":"28191","DOI":"10.1074\/jbc.M204011200","volume":"277","author":"Falcon-Perez JM","year":"2002","journal-title":"J Biol Chem"},{"key":"B12","first-page":"e2352","volume":"47","author":"Feliciano D","year":"2011","journal-title":"J Vis Exp"},{"key":"B13","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1371\/journal.pbio.0060001","volume":"6","author":"Galletta BJ","year":"2008","journal-title":"PLoS Biol"},{"key":"B14","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.ceb.2009.01.006","volume":"21","author":"Galletta BJ","year":"2009","journal-title":"Curr Opin Cell Biol"},{"key":"B15","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1083\/jcb.153.3.627","volume":"153","author":"Goode BL","year":"2001","journal-title":"J Cell Biol"},{"key":"B16","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1083\/jcb.122.3.635","volume":"122","author":"Holtzman DA","year":"1993","journal-title":"J Cell Biol"},{"key":"B17","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1083\/jcb.200110027","volume":"157","author":"Howard JP","year":"2002","journal-title":"J Cell Biol"},{"key":"B18","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1083\/jcb.200708060","volume":"180","author":"Idrissi FZ","year":"2008","journal-title":"J Cell Biol"},{"key":"B19","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/nsmb.1587","volume":"16","author":"Ismail AM","year":"2009","journal-title":"Nat Struct Mol Biol"},{"key":"B20","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1128\/JB.153.1.163-168.1983","volume":"153","author":"Ito H","year":"1983","journal-title":"J Bacteriol"},{"key":"B21","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/S0092-8674(03)00883-3","volume":"115","author":"Kaksonen M","year":"2003","journal-title":"Cell"},{"key":"B22","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.cell.2005.09.024","volume":"123","author":"Kaksonen M","year":"2005","journal-title":"Cell"},{"key":"B23","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1083\/jcb.136.3.649","volume":"136","author":"Li R","year":"1997","journal-title":"J Cell Biol"},{"key":"B24","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1038\/sj.emboj.7601646","volume":"26","author":"Mahadev RK","year":"2007","journal-title":"EMBO J"},{"key":"B25","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1242\/jcs.114.7.1253","volume":"114","author":"Mayer BJ","year":"2001","journal-title":"J Cell Sci"},{"key":"B26","doi-asserted-by":"crossref","first-page":"1890","DOI":"10.1016\/j.cub.2010.10.016","volume":"20","author":"Michelot A","year":"2010","journal-title":"Curr Biol"},{"key":"B27","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.molcel.2008.10.012","volume":"32","author":"Padrick SB","year":"2008","journal-title":"Mol Cell"},{"key":"B28","doi-asserted-by":"crossref","first-page":"E472","DOI":"10.1073\/pnas.1100236108","volume":"108","author":"Padrick SB","year":"2011","journal-title":"Proc Natl Acad Sci USA"},{"key":"B29","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1146\/annurev.biochem.77.060407.135452","volume":"79","author":"Padrick SB","year":"2010","journal-title":"Annu Rev Biochem"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-08-0721"},{"key":"B31","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1146\/annurev.biophys.29.1.545","volume":"29","author":"Pollard TD","year":"2000","journal-title":"Annu Rev Biophys Biomol Struct"},{"key":"B32","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1083\/jcb.120.1.55","volume":"120","author":"Raths S","year":"1993","journal-title":"J Cell Biol"},{"key":"B33","doi-asserted-by":"crossref","first-page":"3103","DOI":"10.1038\/emboj.2009.248","volume":"28","author":"Reider A","year":"2009","journal-title":"EMBO J"},{"key":"B34","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1038\/13732","volume":"17","author":"Rigaut G","year":"1999","journal-title":"Nat Biotechnol"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e08-09-0982"},{"key":"B36","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1016\/S0960-9822(03)00383-X","volume":"13","author":"Rodal AA","year":"2003","journal-title":"Curr Biol"},{"key":"B37","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/0006-291X(65)90152-X","volume":"19","author":"Siegel LM","year":"1965","journal-title":"Biochem Biophys Res Commun"},{"key":"B38","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1093\/genetics\/122.1.19","volume":"122","author":"Sikorski RS","year":"1989","journal-title":"Genetics"},{"key":"B39","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1083\/jcb.200502053","volume":"170","author":"Sirotkin V","year":"2005","journal-title":"J Cell Biol"},{"key":"B40","doi-asserted-by":"crossref","first-page":"28393","DOI":"10.1074\/jbc.M402513200","volume":"279","author":"Starcevic M","year":"2004","journal-title":"J Biol Chem"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-05-0429"},{"key":"B42","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.devcel.2006.05.008","volume":"11","author":"Sun Y","year":"2006","journal-title":"Dev Cell"},{"key":"B43","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/nrm2069","volume":"8","author":"Takenawa T","year":"2007","journal-title":"Nat Rev Mol Cell Biol"},{"key":"B44","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1128\/MCB.20.1.12-25.2000","volume":"20","author":"Tang HY","year":"2000","journal-title":"Mol Cell Biol"},{"key":"B45","doi-asserted-by":"crossref","first-page":"e1000604","DOI":"10.1371\/journal.pbio.1000604","volume":"9","author":"Taylor MJ","year":"2011","journal-title":"PLoS Biol"},{"key":"B46","doi-asserted-by":"crossref","first-page":"E463","DOI":"10.1073\/pnas.1100125108","volume":"108","author":"Ti SC","year":"2011","journal-title":"Proc Natl Acad Sci USA"},{"key":"B47","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1126\/science.1064987","volume":"295","author":"Tong AH","year":"2002","journal-title":"Science"},{"key":"B48","doi-asserted-by":"crossref","first-page":"e1000218","DOI":"10.1371\/journal.pbio.1000218","volume":"7","author":"Tonikian R","year":"2009","journal-title":"PLoS Biol"},{"key":"B50","doi-asserted-by":"crossref","first-page":"5793","DOI":"10.1073\/pnas.0601042103","volume":"103","author":"Toshima JY","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-09-0788"},{"key":"B51","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/nrm2751","volume":"10","author":"Traub LM","year":"2009","journal-title":"Nat Rev Mol Cell Biol"},{"key":"B52","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1083\/jcb.128.5.779","volume":"128","author":"Vida TA","year":"1995","journal-title":"J Cell Biol"},{"key":"B53","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1242\/jcs.115.8.1703","volume":"115","author":"Warren DT","year":"2002","journal-title":"J Cell Sci"},{"key":"B54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.tcb.2011.09.001","volume":"22","author":"Weinberg J","year":"2012","journal-title":"Trends Cell Biol"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T21:28:36Z","timestamp":1643405316000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.e11-12-1022"}},"subtitle":[],"editor":[{"given":"David G.","family":"Drubin","sequence":"additional","affiliation":[{"name":"University of California, Berkeley"}],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,11]]},"references-count":54,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1091\/mbc.e11-12-1022"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e11-12-1022","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11]]}}}