{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:33:56Z","timestamp":1791171236106,"version":"4.1.0"},"reference-count":67,"publisher":"American Society for Cell Biology (ASCB)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p>\n                    Neuronal Wiskott\u2013Aldrich syndrome protein (N-WASP)\u2013activated actin polymerization drives extension of invadopodia and podosomes into the basement layer. In addition to activating Arp2\/3, N-WASP binds actin-filament barbed ends, and both N-WASP and barbed ends are tightly clustered in these invasive structures. We use nanofibers coated with N-WASP WWCA domains as model cell surfaces and single-actin-filament imaging to determine how clustered N-WASP affects Arp2\/3-independent barbed-end assembly. Individual barbed ends captured by WWCA domains grow at or below their diffusion-limited assembly rate. At high filament densities, however, overlapping filaments form buckles between their nanofiber tethers and myosin attachment points. These buckles grew \u223c3.4-fold faster than the diffusion-limited rate of unattached barbed ends. N-WASP constructs with and without the native polyproline (PP) region show similar rate enhancements in the absence of profilin, but profilin slows barbed-end acceleration from constructs containing the PP region. Increasing Mg\n                    <jats:sup>2+<\/jats:sup>\n                    to enhance filament bundling increases the frequency of filament buckle formation, consistent with a requirement of accelerated assembly on barbed-end bundling. We propose that this novel N-WASP assembly activity provides an Arp2\/3-independent force that drives nascent filament bundles into the basement layer during cell invasion.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e12-11-0781","type":"journal-article","created":{"date-parts":[[2013,11,13]],"date-time":"2013-11-13T23:49:04Z","timestamp":1384386544000},"page":"55-65","source":"Crossref","is-referenced-by-count":16,"title":["Processive acceleration of actin barbed-end assembly by N-WASP"],"prefix":"10.1091","volume":"25","author":[{"given":"Nimisha","family":"Khanduja","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey R.","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 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