{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:41:24Z","timestamp":1791240084132,"version":"4.1.0"},"reference-count":71,"publisher":"American Society for Cell Biology (ASCB)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>MicroRNAs (miRNAs) are small, noncoding RNAs involved in posttranscriptional regulation of protein-coding genes in various biological processes. In our preliminary miRNA microarray analysis, miR-375 was identified as the most underexpressed in human oral tumor versus controls. The purpose of the present study is to examine the function of miR-375 as a candidate tumor suppressor miRNA in oral cancer. Cancerous inhibitor of PP2A (CIP2A), a guardian of oncoprotein MYC, is identified as a candidate miR-375 target based on bioinformatics. Luciferase assay accompanied by target sequence mutagenesis elucidates five functional miR-375\u2013binding sites clustered in the CIP2A coding sequence close to the C-terminal domain. Overexpression of CIP2A is clearly demonstrated in oral cancers, and inverse correlation between miR-375 and CIP2A is observed in the tumors, as well as in NCI-60 cell lines, indicating the potential generalized involvement of the miR-375\u2013CIP2A relationship in many other cancers. Transient transfection of miR-375 in oral cancer cells reduces the expression of CIP2A, resulting in decrease of MYC protein levels and leading to reduced proliferation, colony formation, migration, and invasion. Therefore this study shows that underexpression of tumor suppressor miR-375 could lead to uncontrolled CIP2A expression and extended stability of MYC, which contributes to promoting cancerous phenotypes.<\/jats:p>","DOI":"10.1091\/mbc.e12-12-0891","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T07:09:41Z","timestamp":1365059381000},"page":"1638-1648","source":"Crossref","is-referenced-by-count":79,"title":["Tumor suppressor miR-375 regulates MYC expression via repression of CIP2A coding sequence through multiple miRNA\u2013mRNA interactions"],"prefix":"10.1091","volume":"24","author":[{"given":"Hyun Min","family":"Jung","sequence":"first","affiliation":[{"name":"Department of Oral Biology, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rushi S.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brittany L.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald M.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diagnostic Sciences, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Reinhold","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lung-Ji","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Jun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward K. L.","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"4197","DOI":"10.1128\/MCB.00316-10","volume":"30","author":"Abdelmohsen K","year":"2010","journal-title":"Mol Cell Biol"},{"key":"B2","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1038\/nature02871","volume":"431","author":"Ambros V","year":"2004","journal-title":"Nature"},{"key":"B3","doi-asserted-by":"crossref","first-page":"2850","DOI":"10.1158\/1078-0432.CCR-08-3131","volume":"15","author":"Avissar M","year":"2009","journal-title":"Clin Cancer Res"},{"key":"B4","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1038\/nature07242","volume":"455","author":"Baek D","year":"2008","journal-title":"Nature"},{"key":"B5","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","volume":"136","author":"Bartel DP","year":"2009","journal-title":"Cell"},{"key":"B6","doi-asserted-by":"crossref","first-page":"5092","DOI":"10.1158\/1078-0432.CCR-08-3283","volume":"15","author":"Come C","year":"2009","journal-title":"Clin Cancer Res"},{"key":"B7","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.cell.2012.03.003","volume":"149","author":"Dang CV","year":"2012","journal-title":"Cell"},{"key":"B8","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1038\/cr.2010.79","volume":"20","author":"Ding L","year":"2010","journal-title":"Cell Res"},{"key":"B9","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1245\/s10434-010-1313-8","volume":"18","author":"Dong QZ","year":"2011","journal-title":"Ann Surg Oncol"},{"key":"B10","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1261\/rna.972008","volume":"14","author":"Duursma AM","year":"2008","journal-title":"RNA"},{"key":"B11","doi-asserted-by":"crossref","first-page":"R1","DOI":"10.1186\/gb-2003-5-1-r1","volume":"5","author":"Enright AJ","year":"2003","journal-title":"Genome Biol"},{"key":"B12","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/nrc1840","volume":"6","author":"Esquela-Kerscher A","year":"2006","journal-title":"Nat Rev Cancer"},{"key":"B13","doi-asserted-by":"crossref","first-page":"e18067","DOI":"10.1371\/journal.pone.0018067","volume":"6","author":"Fang Z","year":"2011","journal-title":"PLoS One"},{"key":"B14","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1101\/gad.1067003","volume":"17","author":"Fernandez PC","year":"2003","journal-title":"Genes Dev"},{"key":"B15","doi-asserted-by":"crossref","first-page":"14879","DOI":"10.1073\/pnas.0803230105","volume":"105","author":"Forman JJ","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"B16","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1101\/gr.082701.108","volume":"19","author":"Friedman RC","year":"2009","journal-title":"Genome Res"},{"key":"B17","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1002\/hed.10004","volume":"24","author":"Funk GF","year":"2002","journal-title":"Head Neck"},{"key":"B18","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1038\/nrd3179","volume":"9","author":"Garzon R","year":"2010","journal-title":"Nat Rev Drug Discov"},{"key":"B19","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/j.ajpath.2011.12.004","volume":"180","author":"Harris T","year":"2012","journal-title":"Am J Pathol"},{"key":"B20","volume-title":"SEER Cancer Statistics Review, 1975\u20132009","author":"Howlader N","year":"2012"},{"key":"B21","doi-asserted-by":"crossref","first-page":"7539","DOI":"10.1158\/1078-0432.CCR-11-2102","volume":"17","author":"Hui AB","year":"2011","journal-title":"Clin Cancer Res"},{"key":"B22","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3322\/caac.20107","volume":"61","author":"Jemal A","year":"2011","journal-title":"CA Cancer J Clin"},{"key":"B23","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.cell.2005.01.014","volume":"120","author":"Johnson SM","year":"2005","journal-title":"Cell"},{"key":"B24","doi-asserted-by":"crossref","first-page":"29261","DOI":"10.1074\/jbc.M112.366518","volume":"287","author":"Jung HM","year":"2012","journal-title":"J Biol Chem"},{"key":"B25","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.cell.2007.04.044","volume":"130","author":"Junttila MR","year":"2007","journal-title":"Cell"},{"key":"B26","doi-asserted-by":"crossref","first-page":"592","DOI":"10.4161\/cc.7.5.5492","volume":"7","author":"Junttila MR","year":"2008","journal-title":"Cell Cycle"},{"key":"B27","doi-asserted-by":"crossref","first-page":"694","DOI":"10.4161\/cbt.10.7.12895","volume":"10","author":"Katz J","year":"2010","journal-title":"Cancer Biol Ther"},{"key":"B28","doi-asserted-by":"crossref","first-page":"6284","DOI":"10.1093\/nar\/gkh968","volume":"32","author":"Kloosterman WP","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"B29","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1136\/gutjnl-2011-300178","volume":"61","author":"Kong KL","year":"2012","journal-title":"Gut"},{"key":"B30","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/ng1536","volume":"37","author":"Krek A","year":"2005","journal-title":"Nat Genet"},{"key":"B31","doi-asserted-by":"crossref","first-page":"3903","DOI":"10.1073\/pnas.0712321105","volume":"105","author":"Kumar MS","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"B32","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1002\/hep.22256","volume":"47","author":"Ladeiro Y","year":"2008","journal-title":"Hepatology"},{"key":"B33","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nmeth1015","volume":"4","author":"Lee GY","year":"2007","journal-title":"Nat Methods"},{"key":"B34","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cell.2004.12.035","volume":"120","author":"Lewis BP","year":"2005","journal-title":"Cell"},{"key":"B35","doi-asserted-by":"crossref","first-page":"3722","DOI":"10.1158\/1078-0432.CCR-07-4137","volume":"14","author":"Li W","year":"2008","journal-title":"Clin Cancer Res"},{"key":"B36","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nprot.2007.30","volume":"2","author":"Liang CC","year":"2007","journal-title":"Nat Protoc"},{"key":"B37","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1200\/JCO.2005.09.128","volume":"23","author":"Lippman SM","year":"2005","journal-title":"J Clin Oncol"},{"key":"B38","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1126\/science.1076311","volume":"297","author":"Llave C","year":"2002","journal-title":"Science"},{"key":"B39","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1038\/nature03702","volume":"435","author":"Lu J","year":"2005","journal-title":"Nature"},{"key":"B40","doi-asserted-by":"crossref","first-page":"9667","DOI":"10.1073\/pnas.0703820104","volume":"104","author":"Lytle JR","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"B41","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1080\/19424396.2009.12223033","volume":"37","author":"Messadi DV","year":"2009","journal-title":"J Calif Dent Assoc"},{"key":"B42","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1016\/j.cell.2006.07.031","volume":"126","author":"Miranda KC","year":"2006","journal-title":"Cell"},{"key":"B43","doi-asserted-by":"crossref","first-page":"8163","DOI":"10.1093\/nar\/gkr532","volume":"39","author":"Nelson PT","year":"2011","journal-title":"Nucleic Acids Res"},{"key":"B44","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1038\/jhg.2011.66","volume":"56","author":"Nohata N","year":"2011","journal-title":"J Hum Genet"},{"key":"B45","doi-asserted-by":"crossref","first-page":"e16250","DOI":"10.1371\/journal.pone.0016250","volume":"6","author":"Ott CE","year":"2011","journal-title":"PLoS One"},{"key":"B46","doi-asserted-by":"crossref","first-page":"6773","DOI":"10.4049\/jimmunol.0904060","volume":"184","author":"Pan W","year":"2010","journal-title":"J Immunol"},{"key":"B47","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","volume":"29","author":"Pfaffl MW","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"B48","doi-asserted-by":"crossref","first-page":"446","DOI":"10.2174\/156652408785747988","volume":"8","author":"Prochownik EV","year":"2008","journal-title":"Curr Mol Med"},{"key":"B49","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1093\/bioinformatics\/bts043","volume":"28","author":"Reczko M","year":"2012","journal-title":"Bioinformatics"},{"key":"B50","doi-asserted-by":"crossref","first-page":"3499","DOI":"10.1158\/0008-5472.CAN-12-1370","volume":"72","author":"Reinhold WC","year":"2012","journal-title":"Cancer Res"},{"key":"B51","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1038\/bjc.2011.492","volume":"105","author":"Ren J","year":"2011","journal-title":"Br J Cancer"},{"key":"B52","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/S0092-8674(02)00863-2","volume":"110","author":"Rhoades MW","year":"2002","journal-title":"Cell"},{"key":"B53","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1093\/nar\/gkm133","volume":"35","author":"Saetrom P","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"B54","doi-asserted-by":"crossref","first-page":"12085","DOI":"10.1073\/pnas.0905234106","volume":"106","author":"Seike M","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"B55","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1038\/nature07228","volume":"455","author":"Selbach M","year":"2008","journal-title":"Nature"},{"key":"B56","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1002\/pros.20181","volume":"63","author":"Shi FD","year":"2005","journal-title":"Prostate"},{"key":"B57","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1034\/j.1600-0528.2000.280402.x","volume":"28","author":"Shiboski CH","year":"2000","journal-title":"Community Dent Oral Epidemiol"},{"key":"B58","doi-asserted-by":"crossref","first-page":"2799","DOI":"10.1038\/sj.onc.1210083","volume":"26","author":"Si ML","year":"2007","journal-title":"Oncogene"},{"key":"B59","doi-asserted-by":"crossref","first-page":"5006","DOI":"10.1038\/sj.onc.1205625","volume":"21","author":"Soo Hoo L","year":"2002","journal-title":"Oncogene"},{"key":"B60","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev-bioeng-070909-105314","volume":"12","author":"Sun W","year":"2010","journal-title":"Annu Rev Biomed Eng"},{"key":"B61","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1038\/nature07299","volume":"455","author":"Tay Y","year":"2008","journal-title":"Nature"},{"key":"B62","doi-asserted-by":"crossref","first-page":"15472","DOI":"10.1073\/pnas.0707351104","volume":"104","author":"Tazawa H","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"B63","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1158\/0008-5472.CAN-09-2777","volume":"70","author":"Tsukamoto Y","year":"2010","journal-title":"Cancer Res"},{"key":"B64","doi-asserted-by":"crossref","first-page":"e300","DOI":"10.4317\/medoral.16.e300","volume":"16","author":"van der Waal I","year":"2011","journal-title":"Med Oral Patol Oral Cir Bucal"},{"key":"B65","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1101\/gad.1165404","volume":"18","author":"Vella MC","year":"2004","journal-title":"Genes Dev"},{"key":"B66","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1073\/pnas.0510565103","volume":"103","author":"Volinia S","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"key":"B67","first-page":"274","volume":"1775","author":"Voorhoeve PM","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"B68","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.1016\/j.ajpath.2011.07.037","volume":"179","author":"Wang F","year":"2011","journal-title":"Am J Pathol"},{"key":"B69","doi-asserted-by":"crossref","first-page":"13421","DOI":"10.1073\/pnas.0801613105","volume":"105","author":"Yamakuchi M","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"B70","doi-asserted-by":"crossref","first-page":"D202","DOI":"10.1093\/nar\/gkq1056","volume":"39","author":"Yang JH","year":"2011","journal-title":"Nucleic Acids Res"},{"key":"B71","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s10709-009-9378-7","volume":"137","author":"Zhou X","year":"2009","journal-title":"Genetica"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T20:39:54Z","timestamp":1688157594000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.e12-12-0891"}},"subtitle":[],"editor":[{"given":"Kunxin","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,6]]},"references-count":71,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1091\/mbc.e12-12-0891"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e12-12-0891","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6]]}}}