{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:50:57Z","timestamp":1791258657489,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Cell Biology (ASCB)","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:p>CD47 plays an important but incompletely understood role in the innate and adaptive immune responses. CD47, also called integrin-associated protein, has been demonstrated to associate in cis with \u03b21 and \u03b23 integrins. Here we test the hypothesis that CD47 regulates adhesive functions of T-cell \u03b14\u03b21 (VLA-4) and \u03b1L\u03b22 (LFA-1) in in vivo and in vitro models of inflammation. Intravital microscopy studies reveal that CD47<jats:sup>\u2212\/\u2212<\/jats:sup>Th1 cells exhibit reduced interactions with wild-type (WT) inflamed cremaster muscle microvessels. Similarly, murine CD47<jats:sup>\u2212\/\u2212<\/jats:sup>Th1 cells, as compared with WT, showed defects in adhesion and transmigration across tumor necrosis factor-\u03b1 (TNF-\u03b1)\u2013activated murine endothelium and in adhesion to immobilized intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion protein 1 (VCAM-1) under flow conditions. Human Jurkat T-cells lacking CD47 also showed reduced adhesion to TNF-\u03b1\u2013activated endothelium and ICAM-1 and VCAM-1. In cis interactions between Jurkat T-cell \u03b22 integrins and CD47 were detected by fluorescence lifetime imaging microscopy. Unexpectedly, Jurkat CD47 null cells exhibited a striking defect in \u03b21 and \u03b22 integrin activation in response to Mn<jats:sup>2+<\/jats:sup>or Mg<jats:sup>2+<\/jats:sup>\/ethylene glycol tetraacetic acid treatment. Our results demonstrate that CD47 associates with \u03b22 integrins and is necessary to induce high-affinity conformations of LFA-1 and VLA-4 that recognize their endothelial cell ligands and support leukocyte adhesion and transendothelial migration.<\/jats:p>","DOI":"10.1091\/mbc.e13-01-0063","type":"journal-article","created":{"date-parts":[[2013,9,5]],"date-time":"2013-09-05T01:54:26Z","timestamp":1378346066000},"page":"3358-3368","source":"Crossref","is-referenced-by-count":73,"title":["CD47 plays a critical role in T-cell recruitment by regulation of LFA-1 and VLA-4 integrin adhesive functions"],"prefix":"10.1091","volume":"24","author":[{"given":"Veronica","family":"Azcutia","sequence":"first","affiliation":[{"name":"Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115"},{"name":"Harvard Medical School, Boston, MA 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