{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T15:37:16Z","timestamp":1792165036737,"version":"4.4.0"},"reference-count":100,"publisher":"American Society for Cell Biology (ASCB)","issue":"14","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2013,7,15]]},"abstract":"<jats:p>Vesicle biogenesis machinery components such as coat proteins can interact with the actin cytoskeleton for cargo sorting into multiple pathways. It is unknown, however, whether these interactions are a general requirement for the diverse endosome traffic routes. In this study, we identify actin cytoskeleton regulators as previously unrecognized interactors of complexes associated with the Hermansky\u2013Pudlak syndrome. Two complexes mutated in the Hermansky\u2013Pudlak syndrome, adaptor protein complex-3 and biogenesis of lysosome-related organelles complex-1 (BLOC-1), interact with and are regulated by the lipid kinase phosphatidylinositol-4-kinase type II\u03b1 (PI4KII\u03b1). We therefore hypothesized that PI4KII\u03b1 interacts with novel regulators of these complexes. To test this hypothesis, we immunoaffinity purified PI4KII\u03b1 from isotope-labeled cell lysates to quantitatively identify interactors. Strikingly, PI4KII\u03b1 isolation preferentially coenriched proteins that regulate the actin cytoskeleton, including guanine exchange factors for Rho family GTPases such as RhoGEF1 and several subunits of the WASH complex. We biochemically confirmed several of these PI4KII\u03b1 interactions. Of importance, BLOC-1 complex, WASH complex, RhoGEF1, or PI4KII\u03b1 depletions altered the content and\/or subcellular distribution of the BLOC-1\u2013sensitive cargoes PI4KII\u03b1, ATP7A, and VAMP7. We conclude that the Hermansky\u2013Pudlak syndrome complex BLOC-1 and its cargo PI4KII\u03b1 interact with regulators of the actin cytoskeleton.<\/jats:p>","DOI":"10.1091\/mbc.e13-02-0088","type":"journal-article","created":{"date-parts":[[2013,5,16]],"date-time":"2013-05-16T03:31:33Z","timestamp":1368675093000},"page":"2269-2284","source":"Crossref","is-referenced-by-count":87,"title":["The WASH complex, an endosomal Arp2\/3 activator, interacts with the Hermansky\u2013Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type II\u03b1"],"prefix":"10.1091","volume":"24","author":[{"given":"P. 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