{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:35:44Z","timestamp":1791207344587,"version":"4.1.0"},"reference-count":65,"publisher":"American Society for Cell Biology (ASCB)","issue":"17","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>Wiskott-Aldrich syndrome protein and SCAR homologue (WASH) is an important regulator of vesicle trafficking. By generating actin on the surface of intracellular vesicles, WASH is able to directly regulate endosomal sorting and maturation. We report that, in Dictyostelium, WASH is also required for the lysosomal digestion of both phagocytic and autophagic cargo. Consequently, Dictyostelium cells lacking WASH are unable to grow on many bacteria or to digest their own cytoplasm to survive starvation. WASH is required for efficient phagosomal proteolysis, and proteomic analysis demonstrates that this is due to reduced delivery of lysosomal hydrolases. Both protease and lipase delivery are disrupted, and lipid catabolism is also perturbed. Starvation-induced autophagy therefore leads to phospholipid accumulation within WASH-null lysosomes. This causes the formation of multilamellar bodies typical of many lysosomal storage diseases. Mechanistically, we show that, in cells lacking WASH, cathepsin D becomes trapped in a late endosomal compartment, unable to be recycled to nascent phagosomes and autophagosomes. WASH is therefore required for the maturation of lysosomes to a stage at which hydrolases can be retrieved and reused.<\/jats:p>","DOI":"10.1091\/mbc.e13-02-0092","type":"journal-article","created":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T09:24:40Z","timestamp":1374744280000},"page":"2714-2726","source":"Crossref","is-referenced-by-count":95,"title":["WASH is required for lysosomal recycling and efficient autophagic and phagocytic digestion"],"prefix":"10.1091","volume":"24","author":[{"given":"Jason S.","family":"King","sequence":"first","affiliation":[{"name":"Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lie","family":"Gueho","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Hagedorn","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navin","family":"Gopaldass","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Leuba","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Soldati","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Insall","sequence":"additional","affiliation":[{"name":"Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/S0962-8924(00)01794-3","volume":"10","author":"Andrews NW","year":"2000","journal-title":"Trends Cell Biol"},{"key":"B2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1083\/jcb.200312055","volume":"165","author":"Arighi CN","year":"2004","journal-title":"J Cell Biol"},{"key":"B3","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0009-8981(98)00183-1","volume":"280","author":"Bach G","year":"1999","journal-title":"Clin Chim Acta"},{"key":"B4","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1462-5822.2005.00607.x","volume":"8","author":"Benghezal M","year":"2006","journal-title":"Cell Microbiol"},{"key":"B5","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/0-387-30746-X_8","volume-title":"The Prokaryotes","author":"Brisse S","year":"2006"},{"key":"B6","doi-asserted-by":"crossref","first-page":"686","DOI":"10.4161\/auto.6.6.12513","volume":"6","author":"Calvo-Garrido J","year":"2010","journal-title":"Autophagy"},{"key":"B7","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1083\/jcb.201009119","volume":"193","author":"Carnell M","year":"2011","journal-title":"J Cell Biol"},{"key":"B8","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0165-022X(00)00048-8","volume":"42","author":"Chen CS","year":"2000","journal-title":"J Biochem Biophys Methods"},{"key":"B9","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1242\/jcs.115.14.2893","volume":"115","author":"Clarke M","year":"2002","journal-title":"J Cell Sci"},{"key":"B10","doi-asserted-by":"crossref","first-page":"3027","DOI":"10.1128\/JB.184.11.3027-3033.2002","volume":"184","author":"Cosson P","year":"2002","journal-title":"J Bacteriol"},{"key":"B11","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1194\/jlr.M600390-JLR200","volume":"48","author":"Cox BE","year":"2007","journal-title":"J Lipid Res"},{"key":"B12","doi-asserted-by":"crossref","first-page":"39","DOI":"10.2144\/000114054","volume":"55","author":"Davidson AJ","year":"2013","journal-title":"Biotechniques"},{"key":"B13","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1021\/ac702422x","volume":"80","author":"Dayon L","year":"2008","journal-title":"Anal Chem"},{"key":"B14","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.jprot.2009.10.015","volume":"73","author":"Dayon L","year":"2010","journal-title":"J Proteomics"},{"key":"B15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-59745-157-4_1","volume":"445","author":"Deretic V","year":"2008","journal-title":"Methods Mol Biol"},{"key":"B16","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.devcel.2009.09.010","volume":"17","author":"Derivery E","year":"2009","journal-title":"Dev Cell"},{"key":"B17","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1074\/mcp.M112.017608","volume":"11","author":"Dieckmann R","year":"2012","journal-title":"Mol Cell Proteomics"},{"key":"B18","doi-asserted-by":"crossref","first-page":"6565","DOI":"10.1016\/S0021-9258(19)69026-5","volume":"256","author":"Dimond RL","year":"1981","journal-title":"J Biol Chem"},{"key":"B19","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S1074-5521(99)80059-3","volume":"6","author":"Diwu Z","year":"1999","journal-title":"Chem Biol"},{"key":"B20","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1002\/cm.20437","volume":"67","author":"Duleh SN","year":"2010","journal-title":"Cytoskeleton (Hoboken)"},{"key":"B21","doi-asserted-by":"crossref","first-page":"4876","DOI":"10.1093\/hmg\/dds324","volume":"21","author":"Elrick MJ","year":"2012","journal-title":"Hum Mol Genet"},{"key":"B22","doi-asserted-by":"crossref","first-page":"e143","DOI":"10.1093\/nar\/gnh136","volume":"32","author":"Faix J","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"B23","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/nprot.2008.212","volume":"4","author":"Froquet R","year":"2009","journal-title":"Nat Protoc"},{"key":"B24","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1007\/BF00638009","volume":"46","author":"Gerisch G","year":"1959","journal-title":"Naturwissenschaften"},{"key":"B25","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.jprot.2012.12.012","volume":"79","author":"Gluck F","year":"2012","journal-title":"J Proteomics"},{"key":"B26","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1016\/j.devcel.2009.09.009","volume":"17","author":"Gomez TS","year":"2009","journal-title":"Dev Cell"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e12-02-0101"},{"key":"B28","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1600-0854.2011.01296.x","volume":"13","author":"Gopaldass N","year":"2012","journal-title":"Traffic"},{"key":"B29","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1074\/mcp.M600113-MCP200","volume":"5","author":"Gotthardt D","year":"2006","journal-title":"Mol Cell Proteomics"},{"key":"B30","first-page":"439","volume":"346","author":"Gotthardt D","year":"2006","journal-title":"Methods Mol Biol"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-04-0206"},{"key":"B32","first-page":"327","volume":"346","author":"Hagedorn M","year":"2006","journal-title":"Methods Mol Biol"},{"key":"B33","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/S0032-9592(03)00215-2","volume":"39","author":"Han SI","year":"2004","journal-title":"Process Biochem"},{"key":"B34","doi-asserted-by":"crossref","first-page":"3703","DOI":"10.1242\/jcs.071472","volume":"123","author":"Harbour ME","year":"2010","journal-title":"J Cell Sci"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.1.255"},{"key":"B36","doi-asserted-by":"crossref","first-page":"5851","DOI":"10.1046\/j.0014-2956.2001.02530.x","volume":"268","author":"Holopainen JM","year":"2001","journal-title":"Eur J Biochem"},{"key":"B37","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1038\/35044114","volume":"408","author":"Ichimura Y","year":"2000","journal-title":"Nature"},{"key":"B38","doi-asserted-by":"crossref","first-page":"10442","DOI":"10.1073\/pnas.0913293107","volume":"107","author":"Jia D","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"B39","doi-asserted-by":"crossref","first-page":"3833","DOI":"10.1242\/jcs.112.21.3833","volume":"112","author":"Journet A","year":"1999","journal-title":"J Cell Sci"},{"key":"B40","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.molmed.2012.08.002","volume":"18","author":"King JS","year":"2012","journal-title":"Trends Mol Med"},{"key":"B41","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.4161\/auto.7.12.17924","volume":"7","author":"King JS","year":"2011","journal-title":"Autophagy"},{"key":"B42","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1083\/jcb.151.2.263","volume":"151","author":"Kirisako T","year":"2000","journal-title":"J Cell Biol"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e11-03-0193"},{"key":"B44","doi-asserted-by":"crossref","first-page":"e237","DOI":"10.1371\/journal.pgen.0030237","volume":"3","author":"Linardopoulou EV","year":"2007","journal-title":"PLoS Genet"},{"key":"B45","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1038\/nature06639","volume":"451","author":"Mizushima N","year":"2008","journal-title":"Nature"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.01-08-0392"},{"key":"B47","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S1434-4610(99)70026-X","volume":"150","author":"Neuhaus EM","year":"1999","journal-title":"Protist"},{"key":"B48","doi-asserted-by":"crossref","first-page":"17636","DOI":"10.1074\/jbc.M212467200","volume":"278","author":"Otto GP","year":"2003","journal-title":"J Biol Chem"},{"key":"B49","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.devcel.2012.12.014","volume":"24","author":"Park L","year":"2013","journal-title":"Dev Cell"},{"key":"B50","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1128\/MCB.01288-12","volume":"33","author":"Piotrowski JT","year":"2012","journal-title":"Mol Cell Biol"},{"key":"B51","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1247\/csf.27.443","volume":"27","author":"Raposo G","year":"2002","journal-title":"Cell Struct Funct"},{"key":"B52","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1099\/00221287-109-2-225","volume":"109","author":"Ratner DI","year":"1978","journal-title":"J Gen Microbiol"},{"key":"B53","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1007\/978-1-62703-302-2_21","volume":"983","author":"Sattler N","year":"2013","journal-title":"Methods Mol Biol"},{"key":"B54","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.bbalip.2008.12.007","volume":"1791","author":"Schmitz G","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"B55","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1016\/S0022-2275(20)41642-6","volume":"32","author":"Schmitz G","year":"1991","journal-title":"J Lipid Res"},{"key":"B56","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/S0248-4900(00)01102-3","volume":"92","author":"Schneider N","year":"2000","journal-title":"Biol Cell"},{"key":"B57","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1083\/jcb.200312034","volume":"165","author":"Seaman MN","year":"2004","journal-title":"J Cell Biol"},{"key":"B58","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.plasmid.2008.11.003","volume":"61","author":"Veltman DM","year":"2009","journal-title":"Plasmid"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e10-04-0372"},{"key":"B60","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1006\/plas.1994.1054","volume":"32","author":"Wilczynska Z","year":"1994","journal-title":"Plasmid"},{"key":"B61","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1038\/ncb1007-1102","volume":"9","author":"Xie Z","year":"2007","journal-title":"Nat Cell Biol"},{"key":"B62","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/j.1600-0854.2005.00284.x","volume":"6","author":"Yates RM","year":"2005","journal-title":"Traffic"},{"key":"B63","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1093\/oxfordjournals.jbchem.a135438","volume":"98","author":"Yoshida Y","year":"1985","journal-title":"J Biochem"},{"key":"B64","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1083\/jcb.99.3.894","volume":"99","author":"Yumura S","year":"1984","journal-title":"J Cell Biol"},{"key":"B65","doi-asserted-by":"crossref","first-page":"3753","DOI":"10.1242\/jcs.080986","volume":"124","author":"Zech T","year":"2011","journal-title":"J Cell Sci"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T05:14:44Z","timestamp":1715750084000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.e13-02-0092"}},"subtitle":[],"editor":[{"given":"Carole","family":"Parent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":65,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1091\/mbc.e13-02-0092"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e13-02-0092","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.718047158.793483645","asserted-by":"object"}]},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9]]}}}