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Our data reveal that endometrium expresses a higher rate of the fibronectin (FN) extra type III domain A+ (EDA+) splicing isoform, which displays stronger matrix incorporation capacity. We demonstrate that the high FN content of the endometrium matrix, and not specifically the EDA domain, supports trophoblast invasion by showing that forced incorporation of plasma FN (EDA\u2013) promotes efficient trophoblast invasion. We further show that the serine\/arginine-rich protein serine\/arginine-rich splicing factor 1 (SRSF1) is more highly expressed in endometrium and, using RNA interference, that it is involved in the higher EDA exon inclusion rate in endometrium. Our data therefore show a mechanism by which tissues can be distinguished, for their capacity to support invasion, by their different rates of EDA inclusion, linked to their SRSF1 protein levels. In the broader context of cancer pathology, the results suggest that SRSF1 might play a central role not only in the tumor cells, but also in the surrounding stroma.<\/jats:p>","DOI":"10.1091\/mbc.e13-03-0142","type":"journal-article","created":{"date-parts":[[2013,8,22]],"date-time":"2013-08-22T03:59:55Z","timestamp":1377143995000},"page":"3164-3176","source":"Crossref","is-referenced-by-count":22,"title":["Tissue-specific and SRSF1-dependent splicing of fibronectin, a matrix protein that controls host cell invasion"],"prefix":"10.1091","volume":"24","author":[{"given":"Isabel Cristina","family":"Lopez-Mejia","sequence":"first","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique Mol\u00e9culaire de Montpellier, CNRS UMR 5535\/IFR122, Universities of Montpellier 1 and Montpellier 2, 34293 Montpellier Cedex 5, France"},{"name":"D\u00e9partement de Physiologie, Universit\u00e9 de Lausanne, CH-1015 Lausanne, 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