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However, the mechanisms that restrict Par-Tiam1 complex activity to the leading edge to maintain cell polarity during migration remain unclear. We identify the Rac-specific GTPase-activating protein (GAP) breakpoint cluster region protein (Bcr) as a novel regulator of the Par-Tiam1 complex. We show that Bcr interacts with members of the Par complex and inhibits both Rac1 and PKC\u03b6 signaling. Loss of Bcr results in faster, more random migration and striking polarity defects in astrocytes. These polarity defects are rescued by reducing PKC\u03b6 activity or by expressing full-length Bcr, but not an N-terminal deletion mutant or the homologous Rac-GAP, Abr, both of which fail to associate with the Par complex. These results demonstrate that Bcr is an integral member of the Par-Tiam1 complex that controls polarized cell migration by locally restricting both Rac1 and PKC\u03b6 function.<\/jats:p>","DOI":"10.1091\/mbc.e13-06-0333","type":"journal-article","created":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T02:57:00Z","timestamp":1382583420000},"page":"3857-3868","source":"Crossref","is-referenced-by-count":24,"title":["The Rac-GAP Bcr is a novel regulator of the Par complex that controls cell polarity"],"prefix":"10.1091","volume":"24","author":[{"given":"Anjana S.","family":"Narayanan","sequence":"first","affiliation":[{"name":"Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve B.","family":"Reyes","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, University of Texas M.D. Anderson Cancer Center, Houston, TX 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