{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T10:55:07Z","timestamp":1776077707049,"version":"3.50.1"},"reference-count":47,"publisher":"American Society for Cell Biology (ASCB)","issue":"24","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2013,12,15]]},"abstract":"<jats:p> During yeast mating, two haploid nuclei fuse membranes to form a single diploid nucleus. However, the known proteins required for nuclear fusion are unlikely to function as direct fusogens (i.e., they are unlikely to directly catalyze lipid bilayer fusion) based on their predicted structure and localization. Therefore we screened known fusogens from vesicle trafficking (soluble N-ethylmaleimide\u2013sensitive factor attachment protein receptors [SNAREs]) and homotypic endoplasmic reticulum (ER) fusion (Sey1p) for additional roles in nuclear fusion. Here we demonstrate that the ER-localized SNAREs Sec20p, Ufe1p, Use1p, and Bos1p are required for efficient nuclear fusion. In contrast, Sey1p is required indirectly for nuclear fusion; sey1\u0394 zygotes accumulate ER at the zone of cell fusion, causing a block in nuclear congression. However, double mutants of Sey1p and Sec20p, Ufe1p, or Use1p, but not Bos1p, display extreme ER morphology defects, worse than either single mutant, suggesting that retrograde SNAREs fuse ER in the absence of Sey1p. Together these data demonstrate that SNAREs mediate nuclear fusion, ER fusion after cell fusion is necessary to complete nuclear congression, and there exists a SNARE-mediated, Sey1p-independent ER fusion pathway. <\/jats:p>","DOI":"10.1091\/mbc.e13-08-0441","type":"journal-article","created":{"date-parts":[[2013,10,23]],"date-time":"2013-10-23T22:57:00Z","timestamp":1382569020000},"page":"3896-3908","source":"Crossref","is-referenced-by-count":20,"title":["ER-associated SNAREs and Sey1p mediate nuclear fusion at two distinct steps during yeast mating"],"prefix":"10.1091","volume":"24","author":[{"given":"Jason V.","family":"Rogers","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Arlow","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth R.","family":"Inkellis","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy S.","family":"Koo","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark D.","family":"Rose","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1083\/jcb.201111115","volume":"197","author":"Anwar K","year":"2012","journal-title":"J Cell Biol"},{"key":"B2","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1038\/375806a0","volume":"375","author":"Banfield DK","year":"1995","journal-title":"Nature"},{"key":"B3","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.molcel.2008.02.021","volume":"30","author":"Ben-Aroya S","year":"2008","journal-title":"Mol Cell"},{"key":"B4","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1046\/j.1600-0854.2003.00151.x","volume":"5","author":"Burri L","year":"2004","journal-title":"Traffic"},{"key":"B5","doi-asserted-by":"crossref","first-page":"9873","DOI":"10.1073\/pnas.1734000100","volume":"100","author":"Burri L","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"B6","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/j.devcel.2007.04.005","volume":"12","author":"Cai H","year":"2007","journal-title":"Dev Cell"},{"key":"B7","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/ncb2523","volume":"14","author":"Chen S","year":"2012","journal-title":"Nat Cell Biol"},{"key":"B8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ceb.2013.02.006","volume":"25","author":"Chen S","year":"2013","journal-title":"Curr Opin Cell Biol"},{"key":"B9","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.1126\/science.8128252","volume":"263","author":"Cosson P","year":"1994","journal-title":"Science"},{"key":"B11","doi-asserted-by":"crossref","first-page":"3664","DOI":"10.1093\/emboj\/cdg339","volume":"22","author":"Dilcher M","year":"2003","journal-title":"EMBO J"},{"key":"B12","doi-asserted-by":"crossref","first-page":"15781","DOI":"10.1073\/pnas.95.26.15781","volume":"95","author":"Fasshauer D","year":"1998","journal-title":"Proc Natl Acad Sci USA"},{"key":"B13","doi-asserted-by":"crossref","first-page":"2624","DOI":"10.1074\/jbc.273.5.2624","volume":"273","author":"Fischer von Mollard G","year":"1998","journal-title":"J Biol Chem"},{"key":"B14","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/S0076-6879(02)51866-8","volume":"351","author":"Gammie AE","year":"2002","journal-title":"Methods Enzymol"},{"key":"B15","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1242\/jcs.066662","volume":"123","author":"Grote E","year":"2010","journal-title":"J Cell Sci"},{"key":"B16","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1016\/j.cell.2011.11.022","volume":"147","author":"Hu J","year":"2011","journal-title":"Cell"},{"key":"B17","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.cell.2009.05.025","volume":"138","author":"Hu J","year":"2009","journal-title":"Cell"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.11.3849"},{"key":"B19","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/1471-2148-9-19","volume":"9","author":"Kienle N","year":"2009","journal-title":"BMC Evol Biol"},{"key":"B20","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1016\/j.tibs.2010.06.003","volume":"35","author":"Kozlov MM","year":"2010","journal-title":"Trends Biochem Sci"},{"key":"B21","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1083\/jcb.126.4.911","volume":"126","author":"Kurihara LJ","year":"1994","journal-title":"J Cell Biol"},{"key":"B22","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1146\/annurev.cellbio.20.010403.105307","volume":"20","author":"Lee MCS","year":"2004","journal-title":"Annu Rev Cell Dev Biol"},{"key":"B23","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/0092-8674(94)90011-6","volume":"79","author":"Letourneur F","year":"1994","journal-title":"Cell"},{"key":"B24","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0092-8674(00)81097-1","volume":"85","author":"Lewis MJ","year":"1996","journal-title":"Cell"},{"key":"B25","doi-asserted-by":"crossref","first-page":"3017","DOI":"10.1093\/emboj\/16.11.3017","volume":"16","author":"Lewis MJ","year":"1997","journal-title":"EMBO J"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-04-0204"},{"key":"B27","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1038\/35025000","volume":"407","author":"McNew JA","year":"2000","journal-title":"Nature"},{"key":"B28","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.ceb.2005.06.014","volume":"17","author":"Meeusen SL","year":"2005","journal-title":"Curr Opin Cell Biol"},{"key":"B29","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1083\/jcb.200706151","volume":"179","author":"Melloy P","year":"2007","journal-title":"J Cell Biol"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-02-0163"},{"key":"B31","doi-asserted-by":"crossref","first-page":"3405","DOI":"10.1128\/MCB.10.7.3405","volume":"10","author":"Newman AP","year":"1990","journal-title":"Mol Cell Biol"},{"key":"B32","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1038\/nature08280","volume":"460","author":"Orso G","year":"2009","journal-title":"Nature"},{"key":"B33","doi-asserted-by":"crossref","first-page":"6000","DOI":"10.1093\/emboj\/19.22.6000","volume":"19","author":"Ossig R","year":"2000","journal-title":"EMBO J"},{"key":"B34","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1038\/35025076","volume":"407","author":"Parlati F","year":"2000","journal-title":"Nature"},{"key":"B35","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/S0092-8674(00)81129-0","volume":"92","author":"Patel SK","year":"1998","journal-title":"Cell"},{"key":"B36","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1146\/annurev.mi.45.100191.002543","volume":"45","author":"Rose MD","year":"1991","journal-title":"Annu Rev Microbiol"},{"key":"B37","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1146\/annurev.cellbio.12.1.663","volume":"12","author":"Rose MD","year":"1996","journal-title":"Annu Rev Cell Dev Biol"},{"key":"B38","volume-title":"Methods in Yeast Genetics: A Laboratory Course Manual","author":"Rose MD","year":"1990"},{"key":"B39","doi-asserted-by":"crossref","first-page":"17134","DOI":"10.1074\/jbc.272.27.17134","volume":"272","author":"Sacher M","year":"1997","journal-title":"J Biol Chem"},{"key":"B40","doi-asserted-by":"crossref","first-page":"5308","DOI":"10.1128\/MCB.18.9.5308","volume":"18","author":"Sato TK","year":"1998","journal-title":"Mol Cell Biol"},{"key":"B41","volume-title":"Nuclear Envelope Fusion in the Yeast Saccharomyces cerevisiae PhD Thesis.","author":"Shen S","year":"2008"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e08-10-0987"},{"key":"B43","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1083\/jcb.143.3.589","volume":"143","author":"Spang A","year":"1998","journal-title":"J. Cell Biol"},{"key":"B44","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/nature08156","volume":"460","author":"Stein A","year":"2009","journal-title":"Nature"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.7.1175"},{"key":"B46","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/26412","volume":"395","author":"Sutton RB","year":"1998","journal-title":"Nature"},{"key":"B47","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.cell.2005.11.047","volume":"124","author":"Voeltz GK","year":"2006","journal-title":"Cell"},{"key":"B48","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-59745-250-2_1","volume":"475","author":"Ydenberg CA","year":"2008","journal-title":"Methods Mol Biol"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2019,7,14]],"date-time":"2019-07-14T11:10:03Z","timestamp":1563102603000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.e13-08-0441"}},"subtitle":[],"editor":[{"given":"Orna","family":"Cohen-Fix","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,12,15]]},"references-count":47,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2013,12,15]]}},"alternative-id":["10.1091\/mbc.e13-08-0441"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e13-08-0441","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,15]]}}}