{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:52:24Z","timestamp":1790243544791,"version":"4.1.0"},"reference-count":56,"publisher":"American Society for Cell Biology (ASCB)","issue":"14","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2014,7,15]]},"abstract":"<jats:p>The minus end\u2013directed microtubule motor cytoplasmic dynein transports various cellular cargoes, including early endosomes, but how dynein binds to its cargo remains unclear. Recently fungal Hook homologues were found to link dynein to early endosomes for their transport. Here we identified FhipA in Aspergillus nidulans as a key player for HookA (A. nidulans Hook) function via a genome-wide screen for mutants defective in early-endosome distribution. The human homologue of FhipA, FHIP, is a protein in the previously discovered FTS\/Hook\/FHIP (FHF) complex, which contains, besides FHIP and Hook proteins, Fused Toes (FTS). Although this complex was not previously shown to be involved in dynein-mediated transport, we show here that loss of either FhipA or FtsA (A. nidulans FTS homologue) disrupts HookA\u2013early endosome association and inhibits early endosome movement. Both FhipA and FtsA associate with early endosomes, and interestingly, while FtsA\u2013early endosome association requires FhipA and HookA, FhipA\u2013early endosome association is independent of HookA and FtsA. Thus FhipA is more directly linked to early endosomes than HookA and FtsA. However, in the absence of HookA or FtsA, FhipA protein level is significantly reduced. Our results indicate that all three proteins in the FtsA\/HookA\/FhipA complex are important for dynein-mediated early endosome movement.<\/jats:p>","DOI":"10.1091\/mbc.e14-04-0873","type":"journal-article","created":{"date-parts":[[2014,5,29]],"date-time":"2014-05-29T07:56:05Z","timestamp":1401350165000},"page":"2181-2189","source":"Crossref","is-referenced-by-count":67,"title":["FHIP and FTS proteins are critical for dynein-mediated transport of early endosomes in<i>Aspergillus<\/i>"],"prefix":"10.1091","volume":"25","author":[{"given":"Xuanli","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences\u2013F. 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