{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:11:17Z","timestamp":1761919877832},"reference-count":62,"publisher":"American Society for Cell Biology (ASCB)","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:p>Temperature-sensitive cdc1<jats:sup>ts<\/jats:sup>mutants are reported to stop the cell cycle upon a shift to 30\u00b0C in early G2, that is, as small budded cells having completed DNA replication but unable to duplicate the spindle pole body. A recent report showed that PGAP5, a human homologue of CDC1, acts as a phosphodiesterase removing an ethanolamine phosphate (EtN-P) from mannose 2 of the glycosylphosphatidylinositol (GPI) anchor, thus permitting efficient endoplasmic reticulum (ER)-to-Golgi transport of GPI proteins. We find that the essential CDC1 gene can be deleted in mcd4\u2206 cells, which do not attach EtN-P to mannose 1 of the GPI anchor, suggesting that Cdc1 removes the EtN-P added by Mcd4. Cdc1-314<jats:sup>ts<\/jats:sup>mutants do not accumulate GPI proteins in the ER but have a partial secretion block later in the secretory pathway. Growth tests and the genetic interaction profile of cdc1-314<jats:sup>ts<\/jats:sup>pinpoint a distinct cell wall defect. Osmotic support restores GPI protein secretion and actin polarization but not growth. Cell walls of cdc1-314<jats:sup>ts<\/jats:sup>mutants contain large amounts of GPI proteins that are easily released by \u03b2-glucanases and not attached to cell wall \u03b21,6-glucans and that retain their original GPI anchor lipid. This suggests that the presumed transglycosidases Dfg5 and Dcw1 of cdc1-314<jats:sup>ts<\/jats:sup>transfer GPI proteins to cell wall \u03b21,6-glucans inefficiently.<\/jats:p>","DOI":"10.1091\/mbc.e14-06-1033","type":"journal-article","created":{"date-parts":[[2014,8,28]],"date-time":"2014-08-28T06:54:03Z","timestamp":1409208843000},"page":"3375-3388","source":"Crossref","is-referenced-by-count":34,"title":["Cdc1 removes the ethanolamine phosphate of the first mannose of GPI anchors and thereby facilitates the integration of GPI proteins into the yeast cell wall"],"prefix":"10.1091","volume":"25","author":[{"given":"Hector M.","family":"Vazquez","sequence":"first","affiliation":[{"name":"Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Vionnet","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Fribourg, CH-1700 Fribourg, 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