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Using an in vitro bead motility assay, we show that WAVE directly binds VASP, resulting in an increase in Arp2\/3 complex\u2013based actin assembly. We show that this interaction is important in vivo as well, for the formation of lamellipodia during the ventral enclosure event of Caenorhabditis elegans embryogenesis. Ena\/VASP's ability to bind F-actin and profilin-complexed G-actin are important for its effect, whereas Ena\/VASP tetramerization is not necessary. Our data are consistent with the idea that binding of Ena\/VASP to WAVE potentiates Arp2\/3 complex activity and lamellipodial actin assembly.<\/jats:p>","DOI":"10.1091\/mbc.e14-07-1200","type":"journal-article","created":{"date-parts":[[2014,10,30]],"date-time":"2014-10-30T04:29:21Z","timestamp":1414643361000},"page":"55-65","source":"Crossref","is-referenced-by-count":73,"title":["WAVE binds Ena\/VASP for enhanced Arp2\/3 complex\u2013based actin assembly"],"prefix":"10.1091","volume":"26","author":[{"given":"Svitlana","family":"Havrylenko","sequence":"first","affiliation":[{"name":"Institut Curie, Centre de Recherche"},{"name":"Centre National de la Recherche Scientifique, Unit\u00e9 Mixte de Recherche 168"},{"name":"Universit\u00e9 Pierre et Marie Curie, Paris F-75248, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Noguera","sequence":"additional","affiliation":[{"name":"Institut Curie, Centre de Recherche"},{"name":"Centre National de 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