{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:57:37Z","timestamp":1791338257061,"version":"4.3.1"},"reference-count":60,"publisher":"American Society for Cell Biology (ASCB)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>Drosophila immune cells, the hemocytes, undergo four stereotypical developmental migrations to populate the embryo, where they provide immune reconnoitering, as well as a number of non\u2013immune-related functions necessary for proper embryogenesis. Here, we describe a role for Rho1 in one of these developmental migrations in which posteriorly located hemocytes migrate toward the head. This migration requires the interaction of Rho1 with its downstream effector Wash, a Wiskott\u2013Aldrich syndrome family protein. Both Wash knockdown and a Rho1 transgene harboring a mutation that prevents Wash binding exhibit the same developmental migratory defect as Rho1 knockdown. Wash activates the Arp2\/3 complex, whose activity is needed for this migration, whereas members of the WASH regulatory complex (SWIP, Strumpellin, and CCDC53) are not. Our results suggest a WASH complex\u2013independent signaling pathway to regulate the cytoskeleton during a subset of hemocyte developmental migrations.<\/jats:p>","DOI":"10.1091\/mbc.e14-08-1266","type":"journal-article","created":{"date-parts":[[2015,3,5]],"date-time":"2015-03-05T02:32:31Z","timestamp":1425522751000},"page":"1665-1674","source":"Crossref","is-referenced-by-count":24,"title":["Wash functions downstream of Rho1 GTPase in a subset of<i>Drosophila<\/i>immune cell developmental migrations"],"prefix":"10.1091","volume":"26","author":[{"given":"Jeffrey M.","family":"Verboon","sequence":"first","affiliation":[{"name":"Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis K.","family":"Rahe","sequence":"additional","affiliation":[{"name":"Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 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