{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T07:05:15Z","timestamp":1767337515108},"reference-count":47,"publisher":"American Society for Cell Biology (ASCB)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2015,3,15]]},"abstract":"<jats:p>We recently identified a novel GPCR-dependent pathway for regulation of cardiac hypertrophy that depends on Golgi phosphatidylinositol 4-phosphate (PI4P) hydrolysis by a specific isoform of phospholipase C (PLC), PLC\u03b5, at the nuclear envelope. How stimuli are transmitted from cell surface GPCRs to activation of perinuclear PLC\u03b5 is not clear. Here we tested the role of G protein \u03b2\u03b3 subunits. G\u03b2\u03b3 inhibition blocked ET-1\u2013stimulated Golgi PI4P depletion in neonatal and adult ventricular myocytes. Blocking G\u03b2\u03b3 at the Golgi inhibited ET-1\u2013dependent PI4P depletion and nuclear PKD activation. Translocation of G\u03b2\u03b3 to the Golgi stimulated perinuclear Golgi PI4P depletion and nuclear PKD activation. Finally, blocking G\u03b2\u03b3 at the Golgi or PM blocked ET-1\u2013dependent cardiomyocyte hypertrophy. These data indicate that G\u03b2\u03b3 regulation of the perinuclear Golgi PI4P pathway and a separate pathway at the PM is required for ET-1\u2013stimulated hypertrophy, and the efficacy of G\u03b2\u03b3 inhibition in preventing heart failure maybe due in part to its blocking both these pathways.<\/jats:p>","DOI":"10.1091\/mbc.e14-10-1476","type":"journal-article","created":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T05:51:48Z","timestamp":1421905908000},"page":"1188-1198","source":"Crossref","is-referenced-by-count":39,"title":["G protein \u03b2\u03b3 subunits regulate cardiomyocyte hypertrophy through a perinuclear Golgi phosphatidylinositol 4-phosphate hydrolysis pathway"],"prefix":"10.1091","volume":"26","author":[{"given":"S.","family":"Malik","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Physiology, University of Rochester, Rochester, NY 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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