{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:50:30Z","timestamp":1790689830612,"version":"4.1.0"},"reference-count":60,"publisher":"American Society for Cell Biology (ASCB)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:p>Branched actin filament networks in cells are assembled through the combined activities of Arp2\/3 complex and different WASP\/WAVE proteins. Here we used TIRF and electron microscopy to directly compare for the first time the assembly kinetics and architectures of actin filament networks produced by Arp2\/3 complex and dimerized VCA regions of WAVE1, WAVE2, or N-WASP. WAVE1 produced strikingly different networks from WAVE2 or N-WASP, which comprised unexpectedly short filaments. Further analysis showed that the WAVE1-specific activity stemmed from an inhibitory effect on filament elongation both in the presence and absence of Arp2\/3 complex, which was observed even at low stoichiometries of WAVE1 to actin monomers, precluding an effect from monomer sequestration. Using a series of VCA chimeras, we mapped the elongation inhibitory effects of WAVE1 to its WH2 (\u201cV\u201d) domain. Further, mutating a single conserved lysine residue potently disrupted WAVE1's inhibitory effects. Taken together, our results show that WAVE1 has unique activities independent of Arp2\/3 complex that can govern both the growth rates and architectures of actin filament networks. Such activities may underlie previously observed differences between the cellular functions of WAVE1 and WAVE2.<\/jats:p>","DOI":"10.1091\/mbc.e14-10-1477","type":"journal-article","created":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T05:19:40Z","timestamp":1417670380000},"page":"495-505","source":"Crossref","is-referenced-by-count":24,"title":["A novel role for WAVE1 in controlling actin network growth rate and architecture"],"prefix":"10.1091","volume":"26","author":[{"given":"Meredith O.","family":"Sweeney","sequence":"first","affiliation":[{"name":"Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Collins","sequence":"additional","affiliation":[{"name":"Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shae 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